Integrated strategy for the screening of cyclooxygenase‐2 inhibitors from triterpenoid saponins in <scp><i>Clematis tangutica</i></scp>

Wei, Yangfei; Chen, Tao; Wang, Shuo; Shen, Cheng; Song, Zhibo; Li, Aijing; Hong-mei, LI; Li, Yulin

doi:10.1002/pca.3260

Cited by 3 publications

(1 citation statement)

References 63 publications

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“…Despite this, research on Clematis spp. in China has predominantly centered on their medicinal properties [18]. Investigation into their ornamental characteristics is limited to species like Clematis vitalba [19], Clematis crassifolia, and Clematis cadmia [20], found in the southern regions of China.…”

Section: Introductionmentioning

confidence: 99%

Comparison of the Differences in Tolerance to Drought Stress across Five Clematis Species Based on Seed Germination and Seedling Growth

Zhu,

Li,

Liu

et al. 2024

Horticulturae

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Plants of the Clematis genus are attractive ornamental plants due to their various flower colors and patterns, and they play an important role as ground cover plants in landscaping. However, the application of Clematis plants in landscaping in arid and semi-arid areas is limited. This study used five common wild Clematis species in Gansu Province as experimental materials, including Clematistangutica, Clematis glauca, Clematis intricata, Clematis nannophylla, and Clematis fruticosa. By simulating different intensities of drought stress with polyethylene glycol (PEG), the germination behavior of seeds and the physiological and biochemical responses of seedlings of these five species to drought stress were observed. The results showed that 15% PEG stress significantly inhibited the seed germination of the five species, which was also the drought threshold for seed germination of C. fruticosa. C. tangutica exhibited the strongest tolerance to drought stress in seed germination. Seedlings of the five Clematis plants showed different levels of tolerance to drought stress, mainly demonstrating higher tolerance to 10% and 20% concentrations of PEG stress, while a 30% concentration of PEG stress caused varying degrees of damage to the seedlings of the five Clematis species. PCA analysis indicated that seedlings of C. intricata and C. glauca had higher scores under drought stress. These findings can provide a theoretical basis for the selection of urban landscaping plant species in arid and semi-arid regions of northwest China.

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Section: Introductionmentioning

confidence: 99%

Comparison of the Differences in Tolerance to Drought Stress across Five Clematis Species Based on Seed Germination and Seedling Growth

Zhu,

Li,

Liu

et al. 2024

Horticulturae

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Quaternity method for integrated screening, separation, extraction optimization, and bioactivity evaluation of acetylcholinesterase inhibitors from Sophora flavescens Aiton

Zhang,

et al. 2024

Phytochemical Analysis

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IntroductionSophora flavescens Aiton (Fabaceae), a ubiquitous plant species in Asia, contains a wide range of pharmacologically active compounds, such as flavonoids, with potential anti‐Alzheimer's disease (anti‐AD) effects.ObjectivesThe objective of the study is to develop a quaternity method for the screening, isolation, extraction optimization, and activity evaluation of acetylcholinesterase (AChE)‐inhibiting compounds from S. flavescens to realize high‐throughput screening of active substances in traditional Chinese medicine and to provide experimental data for the development of anti‐AD drugs.MethodsWith AChE as the target molecule, affinity ultrafiltration and liquid chromatography‐mass spectrometry were applied to screen for potential inhibitors of the enzyme in S. flavescens. Orthogonal array experiments combined with the multi‐objective Non‐Dominated Sorting Genetic Algorithm III was used for the first time to optimize the process for extracting the active substances. Enzyme inhibition kinetics and molecular docking studies were performed to verify the potential anti‐AD effects of the active compounds.ResultsFive AChE‐inhibiting compounds were identified: kushenol I, kurarinone, sophoraflavanone G, isokurarinone, and kushenol E. These were successfully separated at purities of 72.88%, 98.55%, 96.86%, 96.74%, and 95.84%, respectively, using the n‐hexane/ethyl acetate/methanol/water (4.0/5.0/4.0/5.0, v/v/v/v), n‐hexane/ethyl acetate/methanol/water (5.0/5.0/6.0/4.0, v/v/v/v), and n‐hexane/ethyl acetate/methanol/water (4.9/5.1/5.7/4.3, v/v/v/v) mobile phase systems. Enzyme inhibition kinetics revealed that kushenol E had the best inhibitory effect.ConclusionThis study elucidates the mechanism of action of five active AChE inhibitors in S. flavescens and provides a theoretical basis for the screening and development of anti‐AD and other therapeutic drugs.

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Rapidly screening of pancreatic lipase inhibitors from Clematis tangutica using affinity ultrafiltration‐HPLC‐QTOFMS technique combined with targeted separation, in vitro validation, and molecular docking

Wei,

Chen,

Song

et al. 2024

Phytochemical Analysis

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IntroductionScreening of novel pancreatic lipase inhibitors from complex natural products is a meaningful task.ObjectivesThrough accurately screening and separating pancreatic lipase inhibitors from Clematis tangutica (C. tangutica), to discover new leading compounds for slimming and accelerate the development and utilization of Tibetan medicine resources.MethodsAn integrated strategy that combines affinity ultrafiltration and high‐performance liquid chromatography‐quadrupole time‐of‐flight mass spectrometry (AU‐HPLC‐QTOFMS), targeted separation, in vitro validation, and molecular docking was developed to screen pancreatic lipase inhibitors from C. tangutica. The AU‐HPLC‐QTOFMS technique was performed to fish for the potential active substances. Macroporous resin, preparative liquid chromatography, and high‐speed countercurrent chromatography were implemented for the accurate and targeted separation of active compounds. The inhibitory activities of target compounds to pancreatic lipase were detected by the inhibition experiments in vitro. The binding affinities and binding sites were analyzed using molecular docking.ResultsA total of eleven kinds of pancreatic lipase inhibitory substances were screened from C. tangutica. Seven triterpenoid saponins were screened for the first time as lipase inhibitors and successfully prepared with purities higher than 97%. Tanguticoside B, clematangoticoside J, hederoside H1, and rutin showed stronger inhibitory effects with IC50 values of 1.539 ± 0.048, 1.661 ± 0.092, 1.793 ± 0.069, and 1.792 ± 0.094 mmol/l. Moreover, they have the lowest ΔG values of −10.84, −9.97, −10.87, and −9.39 kcal/mol to pancreatic lipase.ConclusionThe integrated strategy using AU‐HPLC‐QTOFMS, targeted separation, in vitro validation, and molecular docking was feasible for rapidly screening and directionally isolating pancreatic lipase inhibitors from C. tangutica.

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Integrated strategy for the screening of cyclooxygenase‐2 inhibitors from triterpenoid saponins in Clematis tangutica

Cited by 3 publications

References 63 publications

Comparison of the Differences in Tolerance to Drought Stress across Five Clematis Species Based on Seed Germination and Seedling Growth

Comparison of the Differences in Tolerance to Drought Stress across Five Clematis Species Based on Seed Germination and Seedling Growth

Quaternity method for integrated screening, separation, extraction optimization, and bioactivity evaluation of acetylcholinesterase inhibitors from Sophora flavescens Aiton

Rapidly screening of pancreatic lipase inhibitors from Clematis tangutica using affinity ultrafiltration‐HPLC‐QTOFMS technique combined with targeted separation, in vitro validation, and molecular docking

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