Integrated strain engineering and bioprocessing strategies for high-level bio-based production of 3-hydroxyvalerate in Escherichia coli

Miscevic, Dragan; Mao, Ju‐Yi; Kefale, Teshager; Abedi, Daryoush; Huang, Chih‐Ching; Moo‐Young, Murray; Chou, C. Perry

doi:10.1007/s00253-020-10580-5

Cited by 5 publications

(3 citation statements)

References 47 publications

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“…Furthermore, it is also possible to synthesize 1,4-BDO via direct biocatalytic routes from renewable carbohydrate feedstocks (glucose and sucrose) [73]. It has also been found that an engineered Escherichia coli host enhances the anaerobic operation of the oxidative tricarboxylic acid cycle, thereby generating reducing power to drive the BDO pathway [74]. E. coli produce BDO from glucose, xylose, sucrose, and biomass-derived mixed sugar streams.…”

Section: 2- 14-and 23-butanediol Bioplasticsmentioning

confidence: 99%

Environmental impact of bioplastic use: A review

Atiwesh

Mikhael

Parrish

et al. 2021

Heliyon

213

113

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Throughout their lifecycle, petroleum-based plastics are associated with many environmental problems, including greenhouse gas emissions, persistence in marine and terrestrial environments, pollution, etc. On the other hand, bioplastics form a rapidly growing class of polymeric materials that are commonly presented as alternatives to conventional petroleum-based plastics. However, bioplastics also have been linked to important environmental issues such as greenhouse gas emissions and unfavorable land use change, making it necessary to evaluate the true impact of bioplastic use on the environment. Still, while many reviews discuss bioplastics, few comprehensively and simultaneously address the positives and negatives of bioplastic use for the environment. The primary focus of the present review article is to address this gap in present research. To this end, this review addresses the following questions: (1) what are the different types of bioplastics that are currently in commercial use or under development in the industry; (2) are bioplastics truly good for the environment; and (3) how can we better resolve the controversial impact of bioplastics on the environment? Overall, studies discussed in this review article show that the harms associated with bioplastics are less severe as compared to conventional plastics. Moreover, as new types of bioplastics are developed, it becomes important that future studies conduct thorough life cycle and land use change analyses to confirm the eco-friendliness of these new materials. Such studies will help policymakers to determine whether the use of new-generation bioplastics is indeed beneficial to the environment.

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Section: 2- 14-and 23-butanediol Bioplasticsmentioning

confidence: 99%

Environmental impact of bioplastic use: A review

Atiwesh

Mikhael

Parrish

et al. 2021

Heliyon

213

113

View full text Add to dashboard Cite

show abstract

“…Eight overexpressed genes involving the methylmalonyl‐CoA pathway combined with overexpression of 6‐dEB synthases realized the production of 6‐dEB at 0.81 mg/L. Similarly, the Perry Chou's group also improved the carbon flux of the methylmalonyl‐CoA pathway for the enhancement of the propionyl‐CoA pool for productions of several important industrial chemicals in E. coli ., such as 3‐HV (Miscevic et al, 2020; Miscevic, Mao, Kefale, et al, 2020), PHBV (Miscevic et al, 2021) and propionate (Miscevic, Mao, Moo‐Young, et al, 2020) Basically, the carbon flux from the tricarboxylic acid (TCA) cycle was redirected into the methylmalonyl‐CoA pathway via succinate. Deleting the subunit A of succinate dehydrogenase complex sdhA to block the transformation of succinate to fumarate combined with the deletion of the repressor iclR to deregulate glyoxylate shunt could improve the pool of succinate (Figure 3b).…”

Section: Development Of Ocfas Synthesis Pathways Through De Novo Synt...mentioning

confidence: 99%

Microbial production of odd‐chain fatty acids

Qin

Wang

et al. 2022

Biotech & Bioengineering

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Odd-chain fatty acids (OcFAs) and their derivatives have attracted much attention due to their beneficial physiological effects and their potential to be alternatives to advanced fuels. However, cells naturally produce even-chain fatty acids (EcFAs) with negligible OcFAs. In the process of biosynthesis of fatty acids (FAs), the acetyl-CoA serves as the starter unit for EcFAs, and propionyl-CoA works as the starter unit for OcFAs. The lack of sufficient propionyl-CoA, the precursor, is usually regarded as the main restriction for large-scale bioproduction of OcFAs. In recent years, synthetic biology strategies have been used to modify several microorganisms to produce more propionyl-CoA that would enable an efficient biosynthesis of OcFAs. This review discusses several reported and potential metabolic pathways for propionyl-CoA biosynthesis, followed by advances in engineering several cell factories for OcFAs production. Finally, trends and challenges of synthetic biology driven OcFAs production are discussed.

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“…Finally, 30.9 g/L of propionate was produced with an overall yield of 49.7% under optimized aeration condition using fed-batch fermentation (Miscevic et al, 2020b). Subsequently, 3-hydroxyvalerate was produced through condensation of propionyl-CoA and acetyl-CoA via a heterologous pathway using glycerol as the carbon source (Miscevic et al, 2020a;Miscevic et al, 2020c). The effects of aeration condition on activity of GS, and thus, flux distribution into the Sbm pathway were further demonstrated during production of poly (3hydroxybutyrate-co-3-hydroxyvalerate) (Miscevic et al, 2021a).…”

Section: Production Of Succinyl-coa Derivativesmentioning

confidence: 99%

Engineering the glyoxylate cycle for chemical bioproduction

Yang

Liu

Chen

et al. 2022

Front. Bioeng. Biotechnol.

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With growing concerns about environmental issues and sustainable economy, bioproduction of chemicals utilizing microbial cell factories provides an eco-friendly alternative to current petro-based processes. Creating high-performance strains (with high titer, yield, and productivity) through metabolic engineering strategies is critical for cost-competitive production. Commonly, it is inevitable to fine-tuning or rewire the endogenous or heterologous pathways in such processes. As an important pathway involved in the synthesis of many kinds of chemicals, the potential of the glyoxylate cycle in metabolic engineering has been studied extensively these years. Here, we review the metabolic regulation of the glyoxylate cycle and summarize recent achievements in microbial production of chemicals through tuning of the glyoxylate cycle, with a focus on studies implemented in model microorganisms. Also, future prospects for bioproduction of glyoxylate cycle-related chemicals are discussed.

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Integrated strain engineering and bioprocessing strategies for high-level bio-based production of 3-hydroxyvalerate in Escherichia coli

Cited by 5 publications

References 47 publications

Environmental impact of bioplastic use: A review

Environmental impact of bioplastic use: A review

Microbial production of odd‐chain fatty acids

Engineering the glyoxylate cycle for chemical bioproduction

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