Integrated stem cells from apical papilla in a 3D culture system improve human embryonic stem cell derived retinal organoid formation

Savoj, Soraya; Esfahani, Mohammad Hossein Nasr; Karimi, Akbar; Karamali, Fereshteh

doi:10.1016/j.lfs.2021.120273

Cited by 5 publications

(2 citation statements)

References 50 publications

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“…Studies in these protocols continue the isolated optic regions from the adherent cultures to suspension for further culture [ 506 , 507 ]. Some results showed that the direct co-culture of hESCs with different mesenchymal cells could improve the efficiency of optic vesicle and optic cup formation [ 508 ].…”

Section: Current Approaches Of Photoreceptor Regenerationmentioning

confidence: 99%

Potential therapeutic strategies for photoreceptor degeneration: the path to restore vision

et al. 2022

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Photoreceptors (PRs), as the most abundant and light-sensing cells of the neuroretina, are responsible for converting light into electrical signals that can be interpreted by the brain. PR degeneration, including morphological and functional impairment of these cells, causes significant diminution of the retina’s ability to detect light, with consequent loss of vision. Recent findings in ocular regenerative medicine have opened promising avenues to apply neuroprotective therapy, gene therapy, cell replacement therapy, and visual prostheses to the challenge of restoring vision. However, successful visual restoration in the clinical setting requires application of these therapeutic approaches at the appropriate stage of the retinal degeneration. In this review, firstly, we discuss the mechanisms of PR degeneration by focusing on the molecular mechanisms underlying cell death. Subsequently, innovations, recent developments, and promising treatments based on the stage of disorder progression are further explored. Then, the challenges to be addressed before implementation of these therapies in clinical practice are considered. Finally, potential solutions to overcome the current limitations of this growing research area are suggested. Overall, the majority of current treatment modalities are still at an early stage of development and require extensive additional studies, both pre-clinical and clinical, before full restoration of visual function in PR degeneration diseases can be realized. Graphical Abstract

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Section: Current Approaches Of Photoreceptor Regenerationmentioning

confidence: 99%

Potential therapeutic strategies for photoreceptor degeneration: the path to restore vision

et al. 2022

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“…Many studies have proven that MSCs derived from oral cavity can promote not only dental tissue regeneration, including dental pulp, 6 periodontal tissue, 7 and dentin, 8 but also bone 9 and nerve. 10 These MSC resources included dental pulp, 11 gingiva, 12 periodontal ligament, 13 exfoliated deciduous teeth, 14 apical papilla, 15 dental follicle, 16 hard palatal adipose tissue, 17 and hard palatal periosteum, 18 and so on. However, to the best of our knowledge, no studies have reported MSCs derived from a rat hard palate (palatal‐derived mesenchymal stem cells [PMSCs]).…”

Section: Introductionmentioning

confidence: 99%

Light‐controlled scaffold‐ and serum‐free hard palatal‐derived mesenchymal stem cell aggregates for bone regeneration

Jiang

Shao

et al. 2022

Bioengineering & Transla Med

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Cell aggregates that mimic in vivo cell–cell interactions are promising and powerful tools for tissue engineering. This study isolated a new, easily obtained, population of mesenchymal stem cells (MSCs) from rat hard palates named hard palatal‐derived mesenchymal stem cells (PMSCs). The PMSCs were positive for CD90, CD44, and CD29 and negative for CD34, CD45, and CD146. They exhibited clonogenicity, self‐renewal, migration, and multipotent differentiation capacities. Furthermore, this study fabricated scaffold‐free 3D aggregates using light‐controlled cell sheet technology and a serum‐free method. PMSC aggregates were successfully constructed with good viability. Transplantation of the PMSC aggregates and the PMSC aggregate‐implant complexes significantly enhanced bone formation and implant osseointegration in vivo, respectively. This new cell resource is easy to obtain and provides an alternative strategy for tissue engineering and regenerative medicine.

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Therapeutic Potential of Oral-Derived Mesenchymal Stem Cells in Retinal Repair

Mohebichamkhorami,

Niknam,

Zali

et al. 2023

Stem Cell Rev and Rep

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Integrated stem cells from apical papilla in a 3D culture system improve human embryonic stem cell derived retinal organoid formation

Cited by 5 publications

References 50 publications

Potential therapeutic strategies for photoreceptor degeneration: the path to restore vision

Potential therapeutic strategies for photoreceptor degeneration: the path to restore vision

Light‐controlled scaffold‐ and serum‐free hard palatal‐derived mesenchymal stem cell aggregates for bone regeneration

Therapeutic Potential of Oral-Derived Mesenchymal Stem Cells in Retinal Repair

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