Integrated siphon-based metering and sedimentation of whole blood on a hydrophilic lab-on-a-disk

Steigert, J.; Brenner, Thilo; Grumann, M.; Riegger, L.; Lutz, Sascha; Zengerle, Roland; Ducrée, Jens

doi:10.1007/s10544-007-9076-0

Cited by 106 publications

(93 citation statements)

References 21 publications

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“…Current centrifugal platforms, particularly integrated CD-based in vitro diagnostic devices, use surface treatments to implement siphon valving. 2,5,25,26 These siphon microchannels are typically treated by a plasma cleaner, but the induced hydrophilicity changes over time. To avoid surface treatments, Madou et al has proposed the pneumatic pumping technique.…”

Section: B Experimental Resultsmentioning

confidence: 99%

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Euler force actuation mechanism for siphon valving in compact disk-like microfluidic chips

et al. 2014

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Based on the Euler force induced by the acceleration of compact disk (CD)-like microfluidic chip, this paper presents a novel actuation mechanism for siphon valving. At the preliminary stage of acceleration, the Euler force in the tangential direction of CD-like chip takes the primary place compared with the centrifugal force to function as the actuation of the flow, which fills the siphon and actuates the siphon valving. The Euler force actuation mechanism is demonstrated by the numerical solution of the phase-field based mathematical model for the flow in siphon valve. In addition, experimental validation is implemented in the polymethylmethacrylate-based CD-like microfluidic chip manufactured using CO 2 laser engraving technique. To prove the application of the proposed Euler force actuation mechanism, whole blood separation and plasma extraction has been conducted using the Euler force actuated siphon valving. The newly introduced actuation mechanism overcomes the dependence on hydrophilic capillary filling of siphon by avoiding external manipulation or surface treatments of polymeric material. The sacrifice for highly integrated processing in pneumatic pumping technique is also prevented by excluding the volume-occupied compressed air chamber. V C 2014 AIP Publishing LLC. [http://dx

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Section: B Experimental Resultsmentioning

confidence: 99%

“…[1][2][3][4] In CD-like microfluidic chips, siphon valve is one key component used for extraction and metering operation of the liquid phase. 2,5 As presented in Refs. 2 and 5, spontaneous capillary flow is required before rotation of the centrifugal platform and induction of the siphonal flow in siphon valve.…”

Section: Introductionmentioning

confidence: 99%

Euler force actuation mechanism for siphon valving in compact disk-like microfluidic chips

et al. 2014

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“…For use in centrifugal platforms [2], measurements on a substrate rotating at a frequency of up to 60 Hz have been successfully been conducted (Figs. 4 and 5).…”

Section: Resultsmentioning

confidence: 99%

A Simple Opto-Fluidic Switch Detecting Liquid Filling in Polymer-Based Microfluidic Systems

Bundgaard

Geschke

Zengerle

et al. 2007

TRANSDUCERS 2007 - 2007 International Solid-State Sensors, Actuators and Microsystems Conference

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A novel detection scheme for detection of liquid levels and bubbles in microfluidic systems, using the principle of total internal reflection (TIR) is presented. A laser beam impinges on the side walls of a channel which are inclined at 45°. In an unfilled channel of such a "V-groove", TIR deflects the beam by 900 into a simple light detector. Upon the presence of liquid, the refractive index in the channel changes, thus eliminating deflection by TIR. The detection principle is robust, requiring no calibration, and tolerating alignment errors of the laser larger than the width and depth of the microfluidic channels. The machining of the V-groves can seamlessly be integrated into common polymer microfabrication schemes such as injection molding.

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“…Recently, Vella et al(Vella et al 2012) demonstrated an innovative micropatterned paper device for measuring liver function markers from a finger prick of blood. Over the last two decades, various microfluidic "lab-on-a-disc" (LoaD) platforms have proven to facilitate full integration and automation of laboratory unit operations such as blood separation, metering, aliquoting, mixing, reagent storage and sequential reagent delivery Duford et al 2013;Honda et al 2005;Kim et al 2013;Robert et al 2013;Steigert et al 2007; van Oordt et al 2013) for applications in bioprocess, (Nwankire et al 2013) biomedical,(Godino et al 2013Park et al 2012) food allergen (Tortajada-Genaro et al 2011), pathogen detection(Golden et al 2013) and environmental monitoring. (Czugala et al 2012;Hwang et al 2013) Nevertheless, few examples of portable sample-to-answer systems with optical read-out have been demonstrated.…”

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confidence: 99%

A portable centrifugal analyser for liver function screening

Nwankire

Czugala

Burger

et al. 2014

Biosensors and Bioelectronics

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Mortality rates of up to 50% have been reported after liver failure due to drug-induced hepatotoxicity and certain viral infections (Gao et al. 2008). These adverse conditions frequently affect HIV and tuberculosis patients on regular medication in resource-poor settings. Here, we report full integration of sample preparation with read-out of a 5-parameter liver assay panel (LAP) on a portable, easy-to-use, fast and costefficient centrifugal microfluidic analysis system (CMAS). Our unique, dissolvable-film based centrifugopneumatic valving was employed to provide sample-to-answer fashion automation for plasma extraction (from finger-prick of blood), metering and aliquoting into separate reaction chambers for parallelized colorimetric quantification during rotation. The entire LAP completes in less than 20 minutes while using only a tenth the reagent volumes when compared with standard hospital laboratory tests. Accuracy of in-situ liver function screening was validated by 96 separate tests with an average coefficient of variance (CV) of 7.9% compared to benchtop and hospital lab tests. Unpaired two sample statistical t-tests were used to compare the means of CMAS and benchtop reader, on one hand; and CMAS and hospital tests on the other. The results demonstrate no statistical difference between the respective means with 94% and 92% certainty of equivalence, respectively. The portable platform thus saves significant time, labour and costs compared to established technologies, and therefore comply with typical restrictions on lab infrastructure, maintenance, operator skill and costs prevalent in many field clinics of the developing world. It has been successfully deployed in a centralised lab in Nigeria. IntroductionLiver is the largest solid organ in the body and is largely responsible for metabolism and detoxification. (Gao et al. 2008) Liver function tests are widely used in clinical chemistry to assess therapeutic effects and potential medication-induced liver damage, especially when taking medications for HIV, tuberculosis and cancer. (Landis et al. 2013;Rahmioglu et al. 2009; Vella et al. 2012) Literature reports suggest that a mortality rate of 2 -28% can be linked with medication-induced liver damage. (Vella et al. 2012) As a result, monitoring of liver function when on certain medications has become common practice in developed countries but can be expensive in poor resource areas. This has prompted local governments and international funding agencies to set up centralised laboratories for liver function monitoring tests especially for HIV patients. Nonetheless, transport logistics and accessibility remains a significant challenge for the majority of these patients. Thus it is very important to develop portable point-of-care (PoC) devices that could be used for liver function screening in the field. Currently, there are only a handful of sample-to-answer PoC devices available for deployment in the field. Recently, Vella et al.(Vella et al. 2012) demonstrated an innovative micropatterned paper device fo...

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Integrated siphon-based metering and sedimentation of whole blood on a hydrophilic lab-on-a-disk

Cited by 106 publications

References 21 publications

Euler force actuation mechanism for siphon valving in compact disk-like microfluidic chips

Euler force actuation mechanism for siphon valving in compact disk-like microfluidic chips

A Simple Opto-Fluidic Switch Detecting Liquid Filling in Polymer-Based Microfluidic Systems

A portable centrifugal analyser for liver function screening

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