Integrated SICM-AFM-optical microscope to measure forces due to hydrostatic pressure applied to a pipette

Pellegrino, Mario; Orsini, Paolo; Pellegrini, Monica; Baschieri, P.; Dinelli, Franco; Petracchi, D.; Tognoni, E.; Ascoli, C.

doi:10.1049/mnl.2011.0670

Cited by 17 publications

(14 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…4,[11][12][13][14] Both techniques have also been combined. 9,15,16 The imaging mechanism in AFM is based on mechanical forces between the sample and the tip of a cantilever, which is used to track the surface of the sample (Fig. 1a).…”

Section: Introductionmentioning

confidence: 99%

Comparison of Atomic Force Microscopy and Scanning Ion Conductance Microscopy for Live Cell Imaging

et al. 2015

View full text Add to dashboard Cite

Atomic force microscopy (AFM) and scanning ion conductance microscopy (SICM) are excellent and commonly used techniques for imaging the topography of living cells with high resolution. We present a direct comparison of AFM and SICM for imaging microvilli, which are small features on the surface of living cells, and for imaging the shape of whole cells. The imaging quality on microvilli increased significantly after cell fixation for AFM, whereas for SICM it remained constant. The apparent shape of whole cells in the case of AFM depended on the imaging force, which deformed the cell. In the case of SICM, cell deformations were avoided, owing to the contact-free imaging mechanism. We estimated that the lateral resolution on living cells is limited by the cell's elastic modulus for AFM, while it is not for SICM. By long-term, time-lapse imaging of microvilli dynamics, we showed that the imaging quality decreased with time for AFM, while it remained constant for SICM.

show abstract

Section: Introductionmentioning

confidence: 99%

Comparison of Atomic Force Microscopy and Scanning Ion Conductance Microscopy for Live Cell Imaging

et al. 2015

View full text Add to dashboard Cite

show abstract

“…For example, SICM-AFM, employs a bent nanopipette to serve as both a cantilever and also as an open channel to probe conductance. This type of device has been used for both conductance-topography [146] and topography-force measurements [147,148]. The use of confocal microscopy in tandem with SICM has allowed the simultaneous collection of sample topography and imaging of the spatial distribution of fluorescent molecules or particles of interest [149].…”

Section: (D) Scanning Electrochemical Microscopy-scanning Ion Conductmentioning

confidence: 99%

Multifunctional scanning ion conductance microscopy

2017

View full text Add to dashboard Cite

Scanning ion conductance microscopy (SICM) is a nanopipette-based technique that has traditionally been used to image topography or to deliver species to an interface, particularly in a biological setting. This article highlights the recent blossoming of SICM into a technique with a much greater diversity of applications and capability that can be used either standalone, with advanced control (potential-time) functions, or in tandem with other methods. SICM can be used to elucidate functional information about interfaces, such as surface charge density or electrochemical activity (ion fluxes). Using a multi-barrel probe format, SICM-related techniques can be employed to deposit nanoscale three-dimensional structures and further functionality is realized when SICM is combined with scanning electrochemical microscopy (SECM), with simultaneous measurements from a single probe opening up considerable prospects for multifunctional imaging. SICM studies are greatly enhanced by finite-element method modelling for quantitative treatment of issues such as resolution, surface charge and (tip) geometry effects. SICM is particularly applicable to the study of living systems, notably single cells, although applications extend to materials characterization and to new methods of printing and nanofabrication. A more thorough understanding of the electrochemical principles and properties of SICM provides a foundation for significant applications of SICM in electrochemistry and interfacial science.

show abstract

“…Therefore, it is well suited to probe soft and responsive surfaces such as those on living cells. The applied pressure is only a few hundred Pa and results from hydrostatic pressure of the fill level of the nanopipette [20]. The ion current drops with probe-sample approach, because the effective area for the ion trajectories becomes smaller, referred to as current squeezing.…”

Section: Introductionmentioning

confidence: 99%

The nanomorphology of cell surfaces of adhered osteoblasts

Voelkner¹,

Wendt²,

Lange³

et al. 2021

Beilstein J. Nanotechnol.

View full text Add to dashboard Cite

The functionality of living cells is inherently linked to subunits with dimensions ranging from several micrometers down to the nanometer scale. The cell surface plays a particularly important role. Electric signaling, including information processing, takes place at the membrane, as well as adhesion and contact. For osteoblasts, adhesion and spreading are crucial processes with regard to bone implants. Here we present a comprehensive characterization of the 3D nanomorphology of living, as well as fixed, osteoblastic cells using scanning ion conductance microscopy (SICM), which is a nanoprobing method that largely avoids mechanical perturbations. Dynamic ruffles are observed, manifesting themselves in characteristic membrane protrusions. They contribute to the overall surface corrugation, which we systematically study by introducing the relative 3D excess area as a function of the projected adhesion area. A clear anticorrelation between the two parameters is found upon analysis of ca. 40 different cells on glass and on amine-covered surfaces. At the rim of lamellipodia, characteristic edge heights between 100 and 300 nm are observed. Power spectral densities of membrane fluctuations show frequency-dependent decay exponents with absolute values greater than 2 on living osteoblasts. We discuss the capability of apical membrane features and fluctuation dynamics in aiding the assessment of adhesion and migration properties on a single-cell basis.

show abstract

Integrated SICM-AFM-optical microscope to measure forces due to hydrostatic pressure applied to a pipette

Cited by 17 publications

References 13 publications

Comparison of Atomic Force Microscopy and Scanning Ion Conductance Microscopy for Live Cell Imaging

Comparison of Atomic Force Microscopy and Scanning Ion Conductance Microscopy for Live Cell Imaging

Multifunctional scanning ion conductance microscopy

The nanomorphology of cell surfaces of adhered osteoblasts

Contact Info

Product

Resources

About