Integrated serum pharmacochemistry, 16S rRNA sequencing and metabolomics to reveal the material basis and mechanism of Yinzhihuang granule against non-alcoholic fatty liver disease

Tan, Yingying; Huang, Zhihong; Liu, Yingying; Li, Xiaojiaoyang; Stalin, Antony; Fan, Xiaotian; Wu, Zhishan; Wu, Chao; Lu, Shan; Zhang, Fanqin; Chen, Meilin; Huang, Jiaqi; Cheng, Guoliang; Li, Bing; Guo, Siyu; Yu, Yang; Zhang, Shuofeng; Wu, Jiarui

doi:10.1016/j.jep.2023.116418

Cited by 8 publications

(5 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Based on the information in Table , 49 compounds from LQZ were absorbed into the bloodstream of mice, indicating their potential role as key components in LQZ . These compounds are predominantly amino acids and their derivatives, alkaloids, lipids, and phenolic compounds.…”

Section: Resultsmentioning

confidence: 99%

“…Based on the information in Table 2 , 49 compounds from LQZ were absorbed into the bloodstream of mice, indicating their potential role as key components in LQZ. 25 These compounds are predominantly amino acids and their derivatives, alkaloids, lipids, and phenolic compounds. After eliminating redundant data, we identified 665 potential targets related to LQZ using the SwissTargetPrediction database.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Liangxue-Qushi-Zhiyang Decoction Ameliorates DNCB-Induced Atopic Dermatitis in Mice through the MAPK Signaling Pathway Based on Network Pharmacology

Zhang,

Niu

et al. 2024

ACS Omega

View full text Add to dashboard Cite

The traditional prescription of Liangxue-Qushi-Zhiyang decoction (LQZ) has been demonstrated to be efficacious in treating atopic dermatitis (AD), a chronic inflammatory skin disorder marked by intense itching, redness, rashes, and skin thickening. Nevertheless, there has been an inadequate systematic exploration of the potential targets, biological processes, and pathways for AD treatment through LQZ. The study objective was to evaluate the efficacy and possible mechanism of LQZ in AD mice. In our study, we identified the primary compounds of LQZ, analyzed hub targets, and constructed a network. Subsequently, the predicted mechanisms of LQZ in AD were experimentally studied and validated in vivo, as determined by network pharmacological analysis. A total of 80 serum components of LQZ were identified through ultra-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UPLC-ESI-MS/MS), among which 49 compounds were absorbed into the bloodstream. Our results indicated that LQZ targets six putative key factors in the MAPK signaling pathway, which play essential roles in AD, namely, EGFR, p-MAPK1/3, p-MAPK14, IL-1β, IL-6, and TNF-α. We observed spleen coefficient, dermatitis scores, and ear thickness were all downregulated in 2,4dinitrochlorobenzene (DNCB)-induced mice after LQZ treatment. Histological analysis of the dorsal and ear skin further revealed that LQZ significantly decreased skin inflammation, epidermal thickness, and mast cell numbers compared to the DNCB group. Our study demonstrated the effectiveness of LQZ in reducing epidermal and dermal damage in a mouse model of AD. Furthermore, our findings suggest that downregulating the MAPK signaling pathway could be a potential therapeutic strategy for the treatment of AD.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Liangxue-Qushi-Zhiyang Decoction Ameliorates DNCB-Induced Atopic Dermatitis in Mice through the MAPK Signaling Pathway Based on Network Pharmacology

Zhang,

Niu

et al. 2024

ACS Omega

View full text Add to dashboard Cite

show abstract

“…Vinaixa et al [ 28 ] considered that animals, suffering from NAFLD, had a higher conversion of PC and PE in liver. Meanwhile, glycerophospholipids and sphingolipids were the important metabolites affecting the development and progression of NAFLD [ 29 ]. In the present study, the concentrations of PC and PE were significantly increased in the HELP group, while decreased significantly in the FRT4M group.…”

Section: Discussionmentioning

confidence: 99%

Lactiplantibacillus plantarum FRT4 attenuates high-energy low-protein diet-induced fatty liver hemorrhage syndrome in laying hens through regulating gut-liver axis

Li,

Cai,

Liu

et al. 2024

J Animal Sci Biotechnol

View full text Add to dashboard Cite

Background Fatty liver hemorrhage syndrome (FLHS) becomes one of the most major factors resulting in the laying hen death for caged egg production. This study aimed to investigate the therapeutic effects of Lactiplantibacillus plantarum (Lp. plantarum) FRT4 on FLHS model in laying hen with a focus on liver lipid metabolism, and gut microbiota. Results The FLHS model of laying hens was established by feeding a high-energy low-protein (HELP) diet, and the treatment groups were fed a HELP diet supplemented with differential proportions of Lp. plantarum FRT4. The results indicated that Lp. plantarum FRT4 increased laying rate, and reduced the liver lipid accumulation by regulating lipid metabolism (lipid synthesis and transport) and improving the gut microbiota composition. Moreover, Lp. plantarum FRT4 regulated the liver glycerophospholipid metabolism. Meanwhile, “gut-liver” axis analysis showed that there was a correlation between gut microbiota and lipid metabolites. Conclusions The results indicated that Lp. plantarum FRT4 improved the laying performance and alleviated FLHS in HELP diet-induced laying hens through regulating “gut-liver” axis. Our findings reveal that glycerophospholipid metabolism could be the underlying mechanism for the anti-FLHS effect of Lp. plantarum FRT4 and for future use of Lp. plantarum FRT4 as an excellent additive for the prevention and mitigation of FLHS in laying hens.

show abstract

“…Finally, the metabolic function of mice was improved. Some scholars (Tan, et al, 2023) applied serum pharmacochemical methods to identify the key components of Yinzhihuang granule (YZHG) granule, and predicted the core targets of YZHG granule in the treatment of NAFLD by systems biological methods. The results showed that YZHG granule might play a therapeutic role in enhancing intestinal barrier and improving intestinal flora destruction.…”

Section: Chinese Patent Medicinementioning

confidence: 99%

Chinese medicine in the treatment of non-alcoholic fatty liver disease based on network pharmacology: a review

Zheng,

Xue,

et al. 2024

Front. Pharmacol.

View full text Add to dashboard Cite

Non-alcoholic fatty liver disease (NAFLD) is a clinicopathological syndrome characterized by abnormalities in hepatic fat deposition, the incidence of which has been increasing year by year in recent years. It has become the largest chronic liver disease globally and one of the important causes of cirrhosis and even primary liver cancer formation. The pathogenesis of NAFLD has not yet been fully clarified. Modern medicine lacks targeted clinical treatment protocols for NAFLD, and most drugs lack efficacy and have high side effects. In contrast, Traditional Chinese Medicine (TCM) has significant advantages in the treatment and prevention of NAFLD, which have been widely recognized by scholars around the world. In recent years, through the establishment of a “medicine-disease-target-pathway” network relationship, network pharmacology can explore the molecular basis of the role of medicines in disease prevention and treatment from various perspectives, predicting the pharmacological mechanism of the corresponding medicines. This approach is compatible with the holistic view and treatment based on pattern differentiation of TCM and has been widely used in TCM research. In this paper, by searching relevant databases such as PubMed, Web of Science, and Embase, we reviewed and analyzed the relevant signaling pathways and specific mechanisms of action of single Chinese medicine, Chinese medicine combinations, and Chinese patent medicine for the treatment of NAFLD in recent years. These related studies fully demonstrated the therapeutic characteristics of TCM with multi-components, multi-targets, and multi-pathways, which provided strong support for the exact efficacy of TCM exerted in the clinic. In conclusion, we believe that network pharmacology is more in line with the TCM mindset of treating diseases, but with some limitations. In the future, we should eliminate the potential risks of false positives and false negatives, clarify the interconnectivity between components, targets, and diseases, and conduct deeper clinical or experimental studies.

show abstract

Integrated serum pharmacochemistry, 16S rRNA sequencing and metabolomics to reveal the material basis and mechanism of Yinzhihuang granule against non-alcoholic fatty liver disease

Cited by 8 publications

References 60 publications

Liangxue-Qushi-Zhiyang Decoction Ameliorates DNCB-Induced Atopic Dermatitis in Mice through the MAPK Signaling Pathway Based on Network Pharmacology

Liangxue-Qushi-Zhiyang Decoction Ameliorates DNCB-Induced Atopic Dermatitis in Mice through the MAPK Signaling Pathway Based on Network Pharmacology

Lactiplantibacillus plantarum FRT4 attenuates high-energy low-protein diet-induced fatty liver hemorrhage syndrome in laying hens through regulating gut-liver axis

Chinese medicine in the treatment of non-alcoholic fatty liver disease based on network pharmacology: a review

Contact Info

Product

Resources

About