Integrated Sample Preparation, Reaction, and Detection on a High-Frequency Centrifugal Microfluidic Platform

Steigert, J.; Grumann, M.; Brenner, Thilo; Mittenbühler, K.; Nann, Thomas; Rühe, Jürgen; Moser, I.; Haeberle, S.; Riegger, L.; Riegler, Jürgen; Bessler, Wolfgang G.; Zengerle, Roland; Ducrée, Jens

doi:10.1016/j.jala.2005.07.002

Cited by 49 publications

(48 citation statements)

References 62 publications

(85 reference statements)

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“…For instance, the platform could be used to measure under continuous mixing, 32,33 during intermediate steps of syphon valving, 34 or for electrochemical titration measurements.…”

Section: Introductionmentioning

confidence: 99%

Integrating electrochemical detection with centrifugal microfluidics for real-time and fully automated sample testing

et al. 2015

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Abstract. Here we present a robust, stable and low-noise experimental set-up for performing electrochemical detection on a centrifugal microfluidic platform. By using a low-noise electronic component (electrical slipring) it is possible to achieve continuous, on-line monitoring of electrochemical experiments, even when the microfluidic disc is spinning at high velocities. Automated sample handling is achieved by designing a microfluidic system to release analyte sequentially, utilizing on-disc passive valving. In addition, the microfluidic system is designed to trap and keep the liquid sample stationary during analysis. In this way it is possible to perform cyclic voltammetry (CV) measurements at varying spin speeds, without altering the electrochemical response. This greatly simplifies the interpretation and quantification of data. Finally, real-time and continuous monitoring of an entire electrochemical experiment, including all intermediate sample handling steps, is demonstrated by amperometric detection of on-disc mixing of analytes (PBS and ferricyanide).

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“…For instance, the platform could be used to measure under continuous mixing, 32,33 during intermediate steps of syphon valving, 34 or for electrochemical titration measurements.…”

Section: Introductionmentioning

confidence: 99%

Integrating electrochemical detection with centrifugal microfluidics for real-time and fully automated sample testing

et al. 2015

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“…The enhanced sensitivity of the method has been shown to be comparable to standard colorimetric, cuvette-based assays, such as those for alcohol, glucose, and hemoglobin levels in serum and whole blood. 65,124,148,149 …”

Section: Optical Detectionmentioning

confidence: 99%

Lab-on-a-CD: A Fully Integrated Molecular Diagnostic System

et al. 2016

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The field of centrifugal microfluidics has experienced tremendous growth during the past 15 years, especially in applications such as lab-on-a-disc (LoD) diagnostics. The strength of LoD systems lies in its potential for development into fully integrated sample-to-answer analysis systems. This review highlights the technologies necessary to develop the next generation of these systems. In addition to outlining valving and other fluid-handling operations, we discuss the recent advances and future outlook in four categories of LoD processes: reagent storage, sample preparation, nucleic acid amplification, and analyte detection strategies.

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“…In spinning disk-type devices, fluids can be pumped toward the rim of the disk at a different flow rates by manipulating spin speed, surface chemical properties, and various chamber and channel geometric details. Separating plasma from whole blood, fluidic mixing, metering of liquid, and signal enhancement can also be integrated into such systems, which have been applied for rapid detection of glucose, hemoglobin, and alcohol in human whole blood [176]. Other sensing platforms can be combined with the fluidic CD format, such as carbon electrodes for dielectrophoresis [177] and whole blood immunoassay [174,178].…”

Section: Pumpmentioning

confidence: 99%