Integrated rapid-diagnostic-test reader platform on a cellphone

Mudanyali, Onur; Dimitrov, Stoyan; Sikora, Uzair; Padmanabhan, Swati; Navruz, İsa; Ozcan, Aydogan

doi:10.1039/c2lc40235a

Cited by 378 publications

(314 citation statements)

References 39 publications

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“…[2][3][4][5][6][7][8][9][10][11][12][13][14][15] Ozcan and co-workers and Henry and co-workers have recently reviewed the literature in the field. [17][18][19] Test strips, however, typically only provide semi-quantitative results; for full quantitation, test kits for liquid samples remain the gold standard.…”

Section: Introductionmentioning

confidence: 99%

Broadly Available Imaging Devices Enable High-Quality Low-Cost Photometry

et al. 2015

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This paper demonstrates that, for applications in resource-limited environments, expensive microplate spectrophotometers-which are used in many central laboratories for parallel measurement of absorbance of samples-can be replaced by photometers based on inexpensive and ubiquitous, consumer electronic devices (e.g., scanners and cell-phone cameras). Two devices-i) a flatbed scanner operating in transmittance mode and ii) a camera-based photometer constructed from a cell phone camera, a planar light source, and a cardboard box-demonstrate the concept. These devices illuminate samples in microtiter plates from one side, and use the RGB-based imaging sensors of the scanner/camera to measure the light transmitted to the other side. The broadband absorbance of samples (RGB-resolved absorbance) can be calculated using the RGB color values of only three pixels per micro-well. Rigorous theoretical analysis establishes a well-defined relationship between the absorbance spectrum of a sample and its corresponding RGB-resolved absorbance. The linearity and precision of measurements performed with these low-cost photometers on different dyes that absorb across the range of the visible spectrum, and chromogenic products of assays (e.g., enzymatic, ELISA) demonstrates that these low-cost photometers can be used reliably in a broad range of chemical and biochemical analyses. The ability to perform accurate measurements of absorbance on liquid samples, in parallel and at low cost, would enable testing, typically reserved for well-equipped clinics and laboratories, to be performed in circumstances where resources and expertise are limited.

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Section: Introductionmentioning

confidence: 99%

Broadly Available Imaging Devices Enable High-Quality Low-Cost Photometry

et al. 2015

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“…The whole process is done locally on the device, and does not require any connectivity. 17 In the low resource setting, this can be especially helpful, where lab tests can be performed cheaply, with minimal hardware costs, on a device that is highly portable. Another example highlighting the advantages of smartphone cameras is the development of software image stabilisers, which use the accelerometers and gyroscopes already included in smartphones to correct image blur resulting from long exposure times.…”

Section: Technology Considerationsmentioning

confidence: 99%

The utility of mHealth in Medical Imaging

Perera¹,

Chakrabarti²

2013

JournalMTM

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“…To date, the use of smartphone cameras to interpret lateral flow tests has focused on individual still image end‐point readings to interpret paper‐based tests for diagnostics,15, 16, 17, 18, 19, 20, 21, 22, 23 chemical sensing,24, 25 and drug monitoring 26, 27. Cameras and smartphones have also been used with other microfluidic techniques to quantify biological reactions, such as the detection of nucleic acid sequences28 and E. coli detection using quantum dots 29.…”

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confidence: 99%

Quantifying Biomolecular Binding Constants using Video Paper Analytical Devices

Miller

Parolo

Turbé

et al. 2018

Chemistry A European J

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A novel ultra‐low‐cost biochemical analysis platform to quantify protein dissociation binding constants and kinetics using paper microfluidics is reported. This approach marries video imaging with one of humankind's oldest materials: paper, requiring no large, expensive laboratory equipment, complex microfluidics or external power. Temporal measurements of nanoparticle–antibody conjugates binding on paper is found to follow the Langmuir Adsorption Model. This is exploited to measure a series of antibody–antigen dissociation constants on paper, showing excellent agreement with a gold‐standard benchtop interferometer. The concept is demonstrated with a camera and low‐end smartphone, 500‐fold cheaper than the reference method, and can be multiplexed to measure ten reactions in parallel. These findings will help to widen access to quantitative analytical biochemistry, for diverse applications spanning disease diagnostics, drug discovery, and environmental analysis in resource‐limited settings.

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Integrated rapid-diagnostic-test reader platform on a cellphone

Cited by 378 publications

References 39 publications

Broadly Available Imaging Devices Enable High-Quality Low-Cost Photometry

Broadly Available Imaging Devices Enable High-Quality Low-Cost Photometry

The utility of mHealth in Medical Imaging

Quantifying Biomolecular Binding Constants using Video Paper Analytical Devices

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