Integrated proteomic and metabolomic analyses reveal significant changes in chloroplasts and mitochondria of pepper (Capsicum annuum L.) during Sclerotium rolfsii infection

Liao, Hongdong; Deng, Xuelei; Wu, Yonghong; Xu, Jianping; Li, Xin; Zhou, Shudong; Li, Xuefeng; C, Zhu; Luo, Feng; Ma, Yanqing; Zheng, Jun

doi:10.1007/s12275-022-1603-4

Cited by 5 publications

(3 citation statements)

References 59 publications

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“…151 Protein expression of phenylpropanoid pathway genes like POD and the carotenoid levels were upregulated during Sclerotium rolfsii infection in C. annuum stem tissues. 152 Defense genes related to biosynthesis of secondary metabolites such as flavonoids were enriched in transcriptomic analysis of resistant C. annuum variety IIVRC-452 in response to anthracnose infection. 153 Higher anthocyanin accumulation was evaluated in Whiteflyaffected C. annuum plants, and a positive correlation between pest index and pigment genes such as CHS and Golden2-like (GLK) was observed.…”

Section: Elicitor Treatment and Mineralmentioning

confidence: 99%

“…Proteomic analysis of Polyphagotarsonemus latus (Broad mite)-infested C. frutescens revealed that several enzymes of the phenylpropanoid and carotenoid biosynthesis were differentially expressed during plant defense response . Protein expression of phenylpropanoid pathway genes like POD and the carotenoid levels were upregulated during Sclerotium rolfsii infection in C. annuum stem tissues . Defense genes related to biosynthesis of secondary metabolites such as flavonoids were enriched in transcriptomic analysis of resistant C. annuum variety IIVRC-452 in response to anthracnose infection …”

Section: Enhancement Of Capsicum Secondary Metabolites Via Abiotic An...mentioning

confidence: 99%

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Genetic Regulation, Environmental Cues, and Extraction Methods for Higher Yield of Secondary Metabolites in Capsicum

Islam

Rawoof

Kumar

et al. 2023

J. Agric. Food Chem.

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Capsicum (chili pepper) is a widely popular and highly consumed fruit crop with beneficial secondary metabolites such as capsaicinoids, carotenoids, flavonoids, and polyphenols, among others. Interestingly, the secondary metabolite profile is a dynamic function of biosynthetic enzymes, regulatory transcription factors, developmental stage, abiotic and biotic environment, and extraction methods. We propose active manipulable genetic, environmental, and extraction controls for the modulation of quality and quantity of desired secondary metabolites in Capsicum species. Specific biosynthetic genes such as Pun (AT3) and AMT in the capsaicinoids pathway and PSY, LCY, and CCS in the carotenoid pathway can be genetically engineered for enhanced production of capsaicinoids and carotenoids, respectively. Generally, secondary metabolites increase with the ripening of the fruit; however, transcriptional regulators such as MYB, bHLH, and ERF control the extent of accumulation in specific tissues. The precise tuning of biotic and abiotic factors such as light, temperature, and chemical elicitors can maximize the accumulation and retention of secondary metabolites in pre-and postharvest settings. Finally, optimized extraction methods such as ultrasonication and supercritical fluid method can lead to a higher yield of secondary metabolites. Together, the integrated understanding of the genetic regulation of biosynthesis, elicitation treatments, and optimization of extraction methods can maximize the industrial production of secondary metabolites in Capsicum.

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Section: Elicitor Treatment and Mineralmentioning

confidence: 99%

Section: Enhancement Of Capsicum Secondary Metabolites Via Abiotic An...mentioning

confidence: 99%

Genetic Regulation, Environmental Cues, and Extraction Methods for Higher Yield of Secondary Metabolites in Capsicum

Islam

Rawoof

Kumar

et al. 2023

J. Agric. Food Chem.

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show abstract

“…This pathogen generates a variety of secondary metabolites that play crucial roles in attaching to plant surfaces and protecting sclerotia against adverse environmental conditions [10]. A recent study suggests that A. rolfsii significantly disrupts proteins and metabolites associated with both photosynthesis and antioxidant mechanisms within chloroplasts and mitochondria, impacting carbohydrate and amino acid metabolism processes [11].…”

Section: Introductionmentioning

confidence: 99%

Zinc and Boron Soil Applications Affect Athelia rolfsii Stress Response in Sugar Beet (Beta vulgaris L.) Plants

Bhadra,

Mahapatra,

Hosenuzzaman

et al. 2023

Plants

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Generation of reactive oxygen species (ROS) constitutes an initial defense approach in plants during pathogen infection. Here, the effects of the two micronutrients, namely, zinc (Zn) and boron (B), on enzymatic and non-enzymatic antioxidant properties, as well as malondialdehyde (MDA) contents in leaves and roots challenged with Athelia rolfsii, which cause root rot disease, were investigated. The findings revealed that Zn and B application to the potting soil alleviated the adverse effect of A. rolfsii on sugar beet plants and increased the chlorophyll content in leaves. The increased enzymatic antioxidant activities such as catalase (CAT), peroxidase (POX), and ascorbate peroxidase (APX), and non-enzymatic antioxidants such as ascorbic acid (AsA) were observed in Zn applied plants compared to both uninoculated and inoculated control plants. A significant rise in CAT activity was noted in both leaves (335.1%) and roots (264.82%) due to the Zn2B1.5 + Ar treatment, in comparison to the inoculated control plants. On the other hand, B did not enhance the activity of any one of them except AsA. Meanwhile, A. rolfsii infection led to the increased accumulation of MDA content both in the leaves and roots of sugar beet plants. Interestingly, reduced MDA content was recorded in leaves and roots treated with both Zn and B. The results of this study demonstrate that both Zn and B played a vital role in A. rofsii tolerance in sugar beet, while Zn enhances antioxidant enzyme activities, B appeared to have a less pronounced effect on modulating the antioxidant system to alleviate the adverse effect of A. rolfsii.

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Integrated pathological, proteomic and metabolomic analyses reveal significant changes of Eriocheir sinensis hepatopancreatic in response to the microsporidian Hepatospora eriocheir infection

Hou

Wang

et al. 2023

Aquaculture

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Integrated proteomic and metabolomic analyses reveal significant changes in chloroplasts and mitochondria of pepper (Capsicum annuum L.) during Sclerotium rolfsii infection

Cited by 5 publications

References 59 publications

Genetic Regulation, Environmental Cues, and Extraction Methods for Higher Yield of Secondary Metabolites in Capsicum

Genetic Regulation, Environmental Cues, and Extraction Methods for Higher Yield of Secondary Metabolites in Capsicum

Zinc and Boron Soil Applications Affect Athelia rolfsii Stress Response in Sugar Beet (Beta vulgaris L.) Plants

Integrated pathological, proteomic and metabolomic analyses reveal significant changes of Eriocheir sinensis hepatopancreatic in response to the microsporidian Hepatospora eriocheir infection

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