Integrated Plasma and Bile Metabolomics Based on an UHPLC-Q/TOF-MS and Network Pharmacology Approach to Explore the Potential Mechanism of Schisandra chinensis-Protection From Acute Alcoholic Liver Injury

Su, Lianlin; Mao, Jun; Hao, Min; Lu, Tulin; Mao, Chunqin; Ji, De; Tong, Huangjin; Fei, Chenghao

doi:10.3389/fphar.2019.01543

Cited by 14 publications

(8 citation statements)

References 47 publications

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“…During that time, we have characterized human (adult) serum [13], cerebrospinal fluid [14], urine [12], saliva [15] and stool samples [16]. Other groups have also analyzed the metabolomes for human breath [17], breast milk [18], bile fluids [19] and hair [20]. We undertook this study to fill in important gaps in our understanding of urinary metabolite concentrations in neonates.…”

Section: Discussionmentioning

confidence: 99%

The Urinary Metabolome of Healthy Newborns

et al. 2020

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The knowledge of normal metabolite values for neonates is key to establishing robust cut-off values to diagnose diseases, to predict the occurrence of new diseases, to monitor a neonate's metabolism, or to assess their general health status. For full term-newborns, many reference biochemical values are available for blood, serum, plasma and cerebrospinal fluid. However, there is a surprising lack of information about normal urine concentration values for a large number of important metabolites in neonates. In the present work, we used targeted tandem mass spectrometry (MS/MS)-based metabolomic assays to identify and quantify 136 metabolites of biomedical interest in the urine from 48 healthy, full-term term neonates, collected in the first 24 h of life. In addition to this experimental study, we performed a literature review (covering the past eight years and over 500 papers) to update the references values in the Human Metabolome Database/Urine Metabolome Database (HMDB/UMDB). Notably, 86 of the experimentally measured urinary metabolites are being reported in neonates/infants for the first time and another 20 metabolites are being reported in human urine for the first time ever. Sex differences were found for 15 metabolites. The literature review allowed us to identify another 78 urinary metabolites with concentration data. As a result, reference concentration values and ranges for 378 neonatal urinary metabolites are now publicly accessible via the HMDB.

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Section: Discussionmentioning

confidence: 99%

The Urinary Metabolome of Healthy Newborns

et al. 2020

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“…According to the variable importance for the project (VIP) of the OPLS-DA model in each group, the differential ions were selected. Referring to the literature on metabolomics, this study set VIP > 1 as the differential ions between the two groups with independent sample t -test, excluding data with p > 0.05 ( Xing et al, 2017 ; Su et al, 2019 ). Finally, the p < 0.05 differential ions were input into the HMDB database, the structure of the compounds according to the first-order mass spectrometry information was predicted, the predicted exogenous compounds were eliminated, and the final selected differential compounds for structure identification were obtained, which are compared with the HMDB database ( http://www.hmdb.ca/ ), METLIN database ( https://www.sisweb.com/software/ms/wiley-metlin.htm ), and KEGG database ( http://www.genome.jp/kegg/ ).…”

Section: Methodsmentioning

confidence: 99%

Section: Biomarker Identification and Metabolic Pathway Analysismentioning

confidence: 99%

“…Ultra-performance liquid chromatography–quadrupole–time-of-flight mass spectrometry (UHPLC-Q/TOF-MS) has the characteristics of high resolution, sensitivity, simplicity, and high-throughput. It has been used to successfully study metabolic changes caused by diseases and drug toxicity ( Su et al, 2019 ). In this study, metabolomics technology based on UHPLC-Q/TOF-MS and multivariate statistics were used to screen out potential biomarkers in blood, urine, and fecal metabolites related to primary dysmenorrhea, analyze related metabolic pathways, and compare the efficacy and mechanism of RCAS and VCAS in the treatment of primary dysmenorrhea.…”

Section: Introductionmentioning

confidence: 99%

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Revealing the mechanism of raw and vinegar-processed Curcuma aromatica Salisb. [Zingiberaceae] regulates primary dysmenorrhea in rats via integrated metabolomics

Tong

Zhang

et al. 2022

Front. Pharmacol.

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Primary dysmenorrhea (PDM) is a common disorder among women around the world. Two processed products of Curcuma aromatica Salisb. [Zingiberaceae] (CAS) are traditional Chinese medicine (TCM) that have long been used to treat gynecological blood stasis syndrome such as primary dysmenorrhea. The mechanisms and active substances of CAS are still largely unknown. The study aimed to establish a rat model of primary dysmenorrhea which investigates the differences between the pharmacodynamics and mechanisms of raw CAS (RCAS) and vinegar-processed CAS (VCAS). Histopathology, cytokinetics, and metabolomics were adopted to evaluate the anti-blood stasis effect of RCAS and VCAS. In metabolomics, endogenous differential metabolites in plasma, urine, and feces are the essential steps to evaluate the effect of RCAS and VCAS. In this study, the rat model of primary dysmenorrhea was successfully established. After RCAS and VCAS intervention, the uterine tissue morphology of dysmenorrhea model rats was improved, and gland hypertrophy and myometrial hyperplasia were reduced as well as neutrophil content. Compared with the RCAS group, the VCAS group had better uterine morphology, few inflammatory factors, and significantly improved amino acid and lipid metabolism. The aforementioned results support the conclusion that VCAS performed better than RCAS in primary dysmenorrhea and that vinegar processing increases the efficacy of CAS.

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“…In this review, we discuss the influence of sample collection pre-analytical handling procedures and storage conditions on the metabolomic profiles of the biological matrices that are most commonly used to investigate liver functions, namely urine, blood, feces, tissues and cells. Of note, metabolic profiling of several other matrices such as bile and ascitic fluids can provide interesting information about liver functions [10,20,21] but will not be further discussed in this review given the paucity of data regarding sample collection and stability.…”

Section: Introductionmentioning

confidence: 99%

Important Considerations for Sample Collection in Metabolomics Studies with a Special Focus on Applications to Liver Functions

Smith

Villaret-Cazadamont

Claus³

et al. 2020

Metabolites

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Metabolomics has found numerous applications in the study of liver metabolism in health and disease. Metabolomics studies can be conducted in a variety of biological matrices ranging from easily accessible biofluids such as urine, blood or feces, to organs, tissues or even cells. Sample collection and storage are critical steps for which standard operating procedures must be followed. Inappropriate sample collection or storage can indeed result in high variability, interferences with instrumentation or degradation of metabolites. In this review, we will first highlight important general factors that should be considered when planning sample collection in the study design of metabolomic studies, such as nutritional status and circadian rhythm. Then, we will discuss in more detail the specific procedures that have been described for optimal pre-analytical handling of the most commonly used matrices (urine, blood, feces, tissues and cells).

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Integrated Plasma and Bile Metabolomics Based on an UHPLC-Q/TOF-MS and Network Pharmacology Approach to Explore the Potential Mechanism of Schisandra chinensis-Protection From Acute Alcoholic Liver Injury

Abstract: potential biomarkers were identified in their bile samples. The biomarkers were mainly related to inflammation and dysfunctions of amino acids and energy metabolism. The recovery of these dysfunctions partly led to the curative effect of SC on ALI.

Cited by 14 publications

References 47 publications

The Urinary Metabolome of Healthy Newborns

The Urinary Metabolome of Healthy Newborns

Revealing the mechanism of raw and vinegar-processed Curcuma aromatica Salisb. [Zingiberaceae] regulates primary dysmenorrhea in rats via integrated metabolomics

Important Considerations for Sample Collection in Metabolomics Studies with a Special Focus on Applications to Liver Functions

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