Integrated physiological and proteomic analysis reveals underlying response and defense mechanisms of Brachypodium distachyon seedling leaves under osmotic stress, cadmium and their combined stresses

Cheng, Zhiwei; Chen, Zi-Yan; Xing, Yan; Bian, Yanwei; Deng, Xiong; Yan, Yueming

doi:10.1016/j.jprot.2017.09.015

Cited by 23 publications

(19 citation statements)

References 63 publications

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“…Down-regulation of malate dehydrogenase (MDH) in the current study was supported by Kamal et al, [65] when he studied salt stress in wheat. The significance of MDH in Brachypodium seedling roots was reported as an adaptation response to a biotic stress by Chen et al [66]. The down-regulation of MDH was observed during the first 3 days, but at 4 days it responded to Cd and osmotic stresses.…”

Section: Resultsmentioning

confidence: 96%

RETRACTED: Morpho-Physiological and Proteomic Analyses of Eucalyptus camaldulensis as a Bioremediator in Copper-Polluted Soil in Saudi Arabia

Alotaibi

Mohammed

Almutairi

et al. 2019

Plants

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The present investigation aimed to assess the impact of copper (Cu) stress on the physiological and proteomic behavior of Eucalyptus camaldulensis. E. camaldulensis is likely a potential phytoremediator in areas vulnerable to Cu contamination, such as the industrial areas of Riyadh. To realize this objective, young seedlings of E. camaldulensis were potted in an open area with soil comprised of clay and sand. Different doses of Cu (30, 50, and 100 µM) were applied to the plants as CuSO4.5H2O for 6 weeks. Plant growth was monitored during the Cu exposure period, and morphological and physiological indicators were measured once a week to determine the growth rates. A proteomics study was also conducted to find out the influence of Cu stress on proteins. Our results showed that growth was negatively affected by Cu treatment, particularly at the highest concentrations. Moreover, using a proteomic analysis showed 26 targets involved in protein expression, which were separated by matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (MALDI-TOF/TOF MS). Elevated levels of Cu increased the expression of 11 proteins and decreased the expression of 15 proteins. Changes were detected in proteins involved in photosynthesis, translation, transcription, metabolism, and antioxidant enzymes. Our findings provided insights into the molecular mechanisms related to Cu stress, in addition to its influence on the morphological and physiological attributes of E. camaldulensis seedlings. This investigation aimed to characterize the mechanism behind the impact of Cu stress on the plant.

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Section: Resultsmentioning

confidence: 96%

RETRACTED: Morpho-Physiological and Proteomic Analyses of Eucalyptus camaldulensis as a Bioremediator in Copper-Polluted Soil in Saudi Arabia

Alotaibi

Mohammed

Almutairi

et al. 2019

Plants

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“…8 ). This is consistent with our recent study showing that GAPDH and TPI were upregulated in leaves under osmotic and Cd 2+ stress and the combined stress 69 . Thus, energy provision is important for plant adaption and defense against abiotic stresses.…”

Section: Discussionsupporting

confidence: 94%

“…A previous study indicated that the growth of Arabidopsis was significantly inhibited after loss of cPGM activity 72 . Our recent study displayed that PGM was upregulated under both osmotic stress, Cd 2+ stress, and combined stress in Brachapodium leaves 69 . In this study, when roots were subjected to the three stresses, PGM was upregulated.…”

Section: Discussionmentioning

confidence: 92%

“…Malate dehydrogenase (MDH, spot 92) can remarkably enhance salt and aluminum toxicity resistance of plants by increasing the levels of malic acid 67 , 68 . A recent study showed that MDH1 was significantly accumulated under Cd 2+ stress 69 . In this study, MDH was downregulated at 1–3 days, but upregulated at day 4 under the three stresses, indexing that MDH could play a role in plant response and adaptation to abiotic stresses.…”

Section: Discussionmentioning

confidence: 98%

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Identification of differentially accumulated proteins involved in regulating independent and combined osmosis and cadmium stress response in Brachypodium seedling roots

Chen

Zhu

et al. 2018

Sci Rep

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In this study, we aimed to identify differentially accumulated proteins (DAPs) involved in PEG mock osmotic stress, cadmium (Cd2+) stress, and their combined stress responses in Brachypodium distachyon seedling roots. The results showed that combined PEG and Cd2+ stresses had more significant effects on Brachypodium seedling root growth, physiological traits, and ultrastructures when compared with each individual stress. Totally, 106 DAPs were identified that are responsive to individual and combined stresses in roots. These DAPs were mainly involved in energy metabolism, detoxification and stress defense and protein metabolism. Principal component analysis revealed that DAPs from Cd2+ and combined stress treatments were grouped closer than those from osmotic stress treatment, indicating that Cd2+ and combined stresses had more severe influences on the root proteome than osmotic stress alone. Protein–protein interaction analyses highlighted a 14-3-3 centered sub-network that synergistically responded to osmotic and Cd2+ stresses and their combined stresses. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis of 14 key DAP genes revealed that most genes showed consistency between transcriptional and translational expression patterns. A putative pathway of proteome metabolic changes in Brachypodium seedling roots under different stresses was proposed, which revealed a complicated synergetic responsive network of plant roots to adverse environments.

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“…27 2-DE is an effective tool for gaining insight into physiological changes at cellular level. 28 Thus analysis and identication of differentially expressed proteins during BDE-209 biodegradation could give us an insight into cell changes in this process. In this experiment, 12 differentially expressed proteins were identied and listed in Table 1.…”

Section: Discussionmentioning

confidence: 99%

Cell changes and differential proteomic analysis during biodegradation of decabromodiphenyl ether (BDE-209) byPseudomonas aeruginosa

Liu

Gong

et al. 2019

RSC Adv.

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Integrated physiological and proteomic analysis reveals underlying response and defense mechanisms of Brachypodium distachyon seedling leaves under osmotic stress, cadmium and their combined stresses

Cited by 23 publications

References 63 publications

RETRACTED: Morpho-Physiological and Proteomic Analyses of Eucalyptus camaldulensis as a Bioremediator in Copper-Polluted Soil in Saudi Arabia

RETRACTED: Morpho-Physiological and Proteomic Analyses of Eucalyptus camaldulensis as a Bioremediator in Copper-Polluted Soil in Saudi Arabia

Identification of differentially accumulated proteins involved in regulating independent and combined osmosis and cadmium stress response in Brachypodium seedling roots

Cell changes and differential proteomic analysis during biodegradation of decabromodiphenyl ether (BDE-209) byPseudomonas aeruginosa

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