Integrated optical output grating coupler as biochemical sensor

Clerc, D.; Lukosz, W.

doi:10.1016/0925-4005(93)01090-q

Cited by 43 publications

(10 citation statements)

References 1 publication

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“…An affinity biosensor consists of a transducer (electrochemical [1], piezoelectric [2], or optical [3]) and a biological recognition element which is able to interact with a selected analyte. Various optical methods have been exploited in biosensors including fluorescence spectroscopy [4], interferometry (reflectometric white light interferometry [5] and modal interferometry in optical waveguide structures [6]), spectroscopy of guided modes of optical waveguides (grating coupler [7] and resonant mirror [8]), and surface plasmon resonance (SPR) [9,10]. Fluorescence-based biosensors offer high sensitivity but, due to the use of labels, they require either multi-step detection protocols or delicately balanced affinities of interacting biomolecules for displacement assays, causing sensor cross-sensitivity to non-target analytes [11].…”

Section: Introductionmentioning

confidence: 99%

Present and future of surface plasmon resonance biosensors

Homola

2003

Analytical and Bioanalytical Chemistry

2,049

1,026

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Surface plasmon resonance (SPR) biosensors are optical sensors exploiting special electromagnetic waves-surface plasmon-polaritons-to probe interactions between an analyte in solution and a biomolecular recognition element immobilized on the SPR sensor surface. Major application areas include detection of biological analytes and analysis of biomolecular interactions where SPR biosensors provide benefits of label-free real-time analytical technology. This paper reviews fundamentals of SPR affinity biosensors and discusses recent advances in development and applications of SPR biosensors.

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Section: Introductionmentioning

confidence: 99%

Present and future of surface plasmon resonance biosensors

Homola

2003

Analytical and Bioanalytical Chemistry

2,049

1,026

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“…During recent years integrated optical sensors have been used extensively in sensitive (bio)chemical analysis. A number of such sensors, e.g., uniform grating couplers 1 and difference, 2 Mach-Zehnder, 3 and Young 4 interferometers, have been proposed and successfully implemented. The principle of these sensors is based on highly accurate measurement of the effective refractive-index change ͑N eff ͒ that occurs as a result of the adsorption of an analyte in the sensing area of a waveguide.…”

mentioning

confidence: 99%

Sensor based on an integrated optical microcavity

et al. 2002

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A novel integrated optical sensor based on a cylindrical microcavity (MC) is proposed. A MC sustains so-called whispering-gallery modes (WGMs), in which the energy of the optical field can be efficiently stored. By monitoring the scattering intensity from the MC, one can detect minute changes in the refractive index of the WGM, for instance, as a result of analyte adsorption. Measurement of a change in refractive index of as little as 10(-4) is demonstrated experimentally. The MC-based integrated optical sensor may have a size of approximately 8mum , and it is rugged and inexpensive.

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“…The SPR biosensor allows direct, real-time, label-free and quantitative detection of interactions by measuring refractive index change at or near a thin metal film surface 10,11 and offer unique opportunity for rapid and cost effective detection and identification of target biomolecules 12 . Antibodies are the most preferred biomolecules for capture of any analyte in a test sample 13 . SPR sensors have been applied to clinical and medical diagnosis 14,15 , with research focusing on developing assays to measure antigen-antibody interactions.…”

Section: Introductionmentioning

confidence: 99%

Surface plasmon resonance immunosensor for label-free detection of BIRC5 biomarker in spontaneously occurring canine mammary tumours

Jena

Shrivastava

Saxena

et al. 2019

Sci Rep

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We report detection of Baculoviral inhibitor of apoptosis repeat containing-5 (BIRC5) protein biomarker in dog serum by label-free surface plasmon resonance (SPR) immunosensor. Initially, overexpression of BIRC5 in canine mammary tumour (CMT) tissues was confirmed by real-time PCR. Recombinant BIRC5 was produced and protein specific antibodies developed in guinea pig specifically reacted with native protein in immunohistochemistry and immunocytochemistry. SPR immunosensor was developed by fabricating anti-BIRC5 antibodies on gold sensor disc. The equilibrium dissociation constant, (KD = kd/ka) was 12.1 × 10−12 M; which indicates that antibodies are of high affinity with sensitivity in picomolar range. The SPR assay could detect as low as 6.25 pg/ml of BIRC5 protein in a calibration experiment (r2 = 0.9964). On testing real clinical samples, 95% specificity and 73.33% sensitivity were recorded. The average amount of serum BIRC5 in dogs with CMT was 110.02 ± 9.77 pg/ml; whereas, in non-cancerous disease conditions, 44.79 ± 4.28 pg/ml and in healthy dog sera 30.28 ± 2.99 pg/ml protein was detected. The SPR immunosensor for detection of BIRC5 in dog sera is reported for the first time and this may find prognostic and diagnostic applications in management of CMT. In future, ‘on-site’ sensors can be developed using this technique for near-patient testing.

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Integrated optical output grating coupler as biochemical sensor

Cited by 43 publications

References 1 publication

Present and future of surface plasmon resonance biosensors

Present and future of surface plasmon resonance biosensors

Sensor based on an integrated optical microcavity

Surface plasmon resonance immunosensor for label-free detection of BIRC5 biomarker in spontaneously occurring canine mammary tumours

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