Integrated network pharmacology and antioxidant activity-guided screen system to exploring antioxidants and quality markers of Shunaoxin pills against chronic cerebral ischemia

Chang, Nianwei; Cheng, Dandan; Ni, Jia-Nan; Guo, Yingying; Chu, Guangcui; Kim, Unchol; Jiang, Min; Bai, Gang

doi:10.4103/wjtcm.wjtcm_5_19

Cited by 4 publications

(1 citation statement)

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“…Circular RNA (circRNA), recognized as a class of non-coding closed-loop RNA molecules without the 5′ end cap and the 3′ poly(A) tail, is not easily degraded by RNA exonucleases [6,7]. Many studies have shown that circRNAs' expression is tissue-specific and developmental stage-specific, closely related to disease development, thus being potential to be biomarkers for diseases [8,9]. For example, the expression of hsa_ circRNA_101996 in cervical cancer is positively associated with TNM stage, tumor size, and lymph node metastasis [10]; circRNA_102171 expression is demonstrably elevated in thyroid cancer, and knockdown of circRNA_102171 dramatically impedes the growth, migration, and invasion of thyroid cancer cells [11].…”

Section: Introductionmentioning

confidence: 99%

Circ_0001658 regulates gefitinib resistance of non-small cell lung cancer through miR-409-3p/TWIST1 axis

Zhang

et al. 2021

Anti-Cancer Drugs

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To investigate the function and mechanism of circular RNA circ_0001658 on gefitinib resistance of non-small cell lung cancer (NSCLC) cells. Circ_0001658, microRNA (miRNA, miR)-409-3p and twist family bHLH transcription factor 1 (TWIST1) expression levels in NSCLC tissues and cell lines were probed by quantitative real-time PCR and Western blot assays. Cell counting kit-8 assay was adopted to examine the inhibitory effect of different concentrations of gefitinib on the viability of NSCLC cells, with the 50% concentration of inhibition (IC 50 ) value calculated. Besides, BrdU assay and flow cytometry assay were used to detect the proliferative and apoptotic rate of NSCLC cells. What's more, the binding relationships between miR-409-3p and circ_0001658, miR-409-3p and TWIST1 mRNA 3′ untranslated region (3′ UTR) were confirmed by dual-luciferase reporter gene assay and RNA immunoprecipitation assay. Circ_0001658 expression was raised in NSCLC tissue samples and cell lines, which was significantly associated with TNM stage and the differentiation degree of NSCLC tissues. Knocking down circ_0001658 could restrain the viability of NSCLC cells, promote the apoptosis, and reduce the IC 50 of gefitinib, while transfection of miR-409-3p inhibitors could partially reverse these impacts. Additionally, circ_0001658 directly targeted miR-409-3p and negatively modulated its expression. TWIST1 was the target of miR-409-3p, which could be indirectly and positively modulated by circ_0001658. Moreover, circ_0001658 expression was negatively interrelated with miR-409-3p expression, while positively correlated with TWIST1 expression in NSCLC samples. Circ_0001658 promotes the malignant phenotypes and the resistance to gefitinib of NSCLC cells by regulating the miR-409-3p/TWIST1 axis.

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Section: Introductionmentioning

confidence: 99%

Circ_0001658 regulates gefitinib resistance of non-small cell lung cancer through miR-409-3p/TWIST1 axis

Zhang

et al. 2021

Anti-Cancer Drugs

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Shunaoxin dropping pill improves cognitive functions of rats with chronic cerebral hypoperfusion via the microbiota-gut-brain axis

Cui

Sun

et al. 2023

Brain Research

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Widely quasi-quantitative analysis of both metabolites and tryptic peptides in animal-originated medicinal materials: Bufonis Venenum as a case

Liao

Qian

et al. 2023

Journal of Pharmaceutical and Biomedical Analysis

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Integrated network pharmacology and antioxidant activity-guided screen system to exploring antioxidants and quality markers of Shunaoxin pills against chronic cerebral ischemia

Cited by 4 publications

References 0 publications

Circ_0001658 regulates gefitinib resistance of non-small cell lung cancer through miR-409-3p/TWIST1 axis

Circ_0001658 regulates gefitinib resistance of non-small cell lung cancer through miR-409-3p/TWIST1 axis

Shunaoxin dropping pill improves cognitive functions of rats with chronic cerebral hypoperfusion via the microbiota-gut-brain axis

Widely quasi-quantitative analysis of both metabolites and tryptic peptides in animal-originated medicinal materials: Bufonis Venenum as a case

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