Integrated multi‐omics analysis uncovers roles of mdm‐miR164b‐MdORE1 in strigolactone‐mediated inhibition of adventitious root formation in apple

Abstract

Apple is one of the most important fruit crops in temperate regions and largely relies on cutting propagation. Adventitious root formation is crucial for the success of cutting propagation. Strigolactones have been reported to function in rooting of woody plants. In this study, we determined that strigolactones have inhibitory effects on adventitious root formation in apple. Transcriptome analysis identified 12 051 differentially expressed genes over the course of adventitious root initiation, with functions related to organogenesis, cell wall biogenesis or plant development. Further analysis indicated that strigolactones might inhibit adventitious root formation through repressing two core hub genes, MdLAC3 and MdORE1. Combining small RNA and degradome sequencing, as well as dual‐luciferase sensor assays, we identified and validated three negatively correlated miRNA–mRNA pairs, including mdm‐miR397–MdLAC3 and mdm‐miR164a/b–MdORE1. Overexpression of mdm‐miR164b and silencing MdORE1 exhibited enhanced adventitious root formation in tobacco and apple, respectively. Finally, we verified the role of mdm‐miR164b–MdORE1 in strigolactone‐mediated repression of rooting ability. Overall, the identified comprehensive regulatory network in apple not only provides insight into strigolactone‐mediated adventitious root formation in other woody plants, but also points to a potential strategy for genetic improvement of rooting capacity in woody plants.

Transcriptomic Database Analysis of Magnesium Transporter (MGT) Gene Family in Pear (Pyrus bretschneideri) Revealed Its Role in Reproductive Stage Development

“…For instance, Our gene PbMGT7 cleaved to miR169 and miR164, whereas PbMGT1 cleaved to miR172, miR173, and miR414 (Figure 11). Enhanced adventitious root production in tobacco (Nicotiana tabacum L.) and apple (Malus domestica) was observed in the silenced MdORE1 and overexpression of mdm-miR164b plants [40]. Evidence from genetic transformations in Arabidopsis lends credence to claims that grape vvi-miR164b is involved in many root development processes [41].…”

miR164‐MhNAC1 regulates apple root nitrogen uptake under low nitrogen stress

“…The miR399‐ PHO2 module responds to low phosphorus stress in maize (Du et al ., 2018); miR444‐ OsMADS27 is involved in the regulation of nitrate‐dependent rice root development (Pachamuthu et al ., 2022). A subset of NAC TFs, including CUC1 , CUC2 , NAC1 , and ORE1 , are regulated by miR164 and participate in plant root development (Laufs et al ., 2004; Guo et al ., 2005; Fan et al ., 2022). In apple, miR164g negatively regulated MsNAC022 to promote root development and enhance active oxygen scavenging capacity, and improve drought tolerance (Peng et al ., 2022).…”