Integrated molecular-network analysis reveals infertility-associated key genes and transcription factors in the non-obstructive azoospermia

Alagundagi, Dhananjay B.; Ghate, Sudeep D.; Shetty, Prakashchandra; Gollapalli, Pavan; Shetty, Praveenkumar; Patil, Prakash

doi:10.1016/j.ejogrb.2023.07.023

Cited by 2 publications

(2 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the context of identifying non-invasive biomarkers for sperm retrieval in cases of NOA, the integration of artificial intelligence (AI) with clinical and molecular data presents a transformative approach ( Figure 1 ). Some studies demonstrate the application of AI in this field, illustrating how machine learning and bioinformatics can significantly enhance the understanding and diagnosis of NOA ( 74 , 75 ). The integration of non-invasive biomarkers into the diagnostic and therapeutic algorithms for NOA patients holds the potential to revolutionize the clinical approach to male infertility, and to transform male infertility management into a realm of personalized medicine.…”

Section: Integrating Non-invasive Biomarkers Into Diagnostic and Ther...mentioning

confidence: 99%

“…Another study ( 75 ) aimed to identifying key genes in male infertility, explored DEGs in NOA by microarray datasets from the NCBI’s gene expression omnibus (GEO) database and hub gene analysis. This study highlighted the importance of bioinformatics in unraveling the molecular mechanisms of NOA.…”

Section: Integrating Non-invasive Biomarkers Into Diagnostic and Ther...mentioning

confidence: 99%

See 1 more Smart Citation

Non-invasive biomarkers for sperm retrieval in non-obstructive patients: a comprehensive review

Fontana,

Sirchia,

Pesenti

et al. 2024

Front. Endocrinol.

View full text Add to dashboard Cite

Recent advancements in reproductive medicine have guided novel strategies for addressing male infertility, particularly in cases of non-obstructive azoospermia (NOA). Two prominent invasive interventions, namely testicular sperm extraction (TESE) and microdissection TESE (micro-TESE), have emerged as key techniques to retrieve gametes for assisted reproduction technologies (ART). Both heterogeneity and complexity of NOA pose a multifaceted challenge to clinicians, as the invasiveness of these procedures and their unpredictable success underscore the need for more precise guidance. Seminal plasma can be aptly regarded as a liquid biopsy of the male reproductive tract, encompassing secretions from the testes, epididymides, seminal vesicles, bulbourethral glands, and prostate. This fluid harbors a variety of cell-free nucleic acids, microvesicles, proteins, and metabolites intricately linked to gonadal activity. However, despite numerous investigations exploring potential biomarkers from seminal fluid, their widespread inclusion into the clinical practice remains limited. This could be partially due to the complex interplay of diverse clinical and genetic factors inherent to NOA that likely contributes to the absence of definitive biomarkers for residual spermatogenesis. It is conceivable that the integration of clinical data with biomarkers could increase the potential in predicting surgical procedure outcomes and their choice in NOA cases. This comprehensive review addresses the challenge of sperm retrieval in NOA through non-invasive biomarkers. Moreover, we delve into promising perspectives, elucidating innovative approaches grounded in multi-omics methodologies, including genomics, transcriptomics and proteomics. These cutting-edge techniques, combined with the clinical and genetics features of patients, could improve the use of biomarkers in personalized medical approaches, patient counseling, and the decision-making continuum. Finally, Artificial intelligence (AI) holds significant potential in the realm of combining biomarkers and clinical data, also in the context of identifying non-invasive biomarkers for sperm retrieval.

show abstract

Section: Integrating Non-invasive Biomarkers Into Diagnostic and Ther...mentioning

confidence: 99%

Section: Integrating Non-invasive Biomarkers Into Diagnostic and Ther...mentioning

confidence: 99%

Non-invasive biomarkers for sperm retrieval in non-obstructive patients: a comprehensive review

Fontana,

Sirchia,

Pesenti

et al. 2024

Front. Endocrinol.

View full text Add to dashboard Cite

show abstract

The Role of Tubulin Polymerization-Promoting Protein2 (TPPP2) in Spermatogenesis: A Narrative Review

Orosz

2024

IJMS

View full text Add to dashboard Cite

Tubulin polymerization-promoting protein2 (TPPP2) is one of the three paralogs of mammalian TPPP proteins. Its possible role in spermatogenesis is described in this narrative review. TPPP2 is expressed specifically in the male reproductive system, mainly in testes and sperm, and also in the epididymis. In testes, TPPP2 is exclusively expressed in elongating spermatids; in the epididymis, it is located in the middle piece of the sperm tail. TPPP2 is involved in spermiogenesis, in steps which are determinative for the formation and morphology of spermatids. The inhibition of TPPP2 decreases sperm motility (the curvilinear velocity of sperms), probably due to influencing mitochondrial energy production since TPPP2 knockout mice possess an impaired mitochondrial structure. There are data on the role of TPPP2 in various mammalian species: human, mouse, swine, and various ruminants; there is a significant homology among TPPP2s from different species. Experiments with Tppp2−/−-mice show that the absence of TPPP2 results in decreased sperm count and serious dysfunction of sperm, including decreased motility; however, the in vitro capacitation and acrosome reaction are not influenced. The symptoms show that Tppp2−/−-mice may be considered as a model for oligoasthenozoospermia.

show abstract

Integrated molecular-network analysis reveals infertility-associated key genes and transcription factors in the non-obstructive azoospermia

Cited by 2 publications

References 46 publications

Non-invasive biomarkers for sperm retrieval in non-obstructive patients: a comprehensive review

Non-invasive biomarkers for sperm retrieval in non-obstructive patients: a comprehensive review

The Role of Tubulin Polymerization-Promoting Protein2 (TPPP2) in Spermatogenesis: A Narrative Review

Contact Info

Product

Resources

About