Integrated miRNA-mRNA analysis reveals the molecular mechanism in mandarin fish (Siniperca chuatsi) in response to fresh baits and artificial diets feeding

Li, Hongyan; Niu, Shuhui; Pan, Houjun; Wang, Guangjun; Xie, Jun; Tian, Jingjing; Zhang, Kai; Xia, Yun; Li, Zhifei; Yu, Ermeng; Gong, Wangbao

doi:10.1016/j.aqrep.2023.101554

Cited by 3 publications

(6 citation statements)

References 43 publications

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“…The significantly lower relative abundance of Actinomycetota observed in mandarin fish fed the AF diet suggested that these fish may have an inferior immune response compared to those in the NF group. This prediction is consistent with our transcriptome analysis results between the two treatments, which showed that the NF group had significantly higher expression of genes associated with TGFβ signaling and tight junctions, indicating enhanced immune and gut barrier functions ( Li et al, 2023 ). Similar findings have been reported in other fish species, such as the freshwater drum ( Aplodinotus grunniens ), which showed a significant down-regulation of Actinobacteriota and Chloroflexi in the gut when fed an artificial diet ( Lozupone et al, 2012 ).…”

Section: Discussionsupporting

confidence: 90%

“…In general, the microbiome diversity of diseased individuals, including mammals and aquatic species, tends to be lower than that of healthy individuals ( Song et al, 2022 ). Therefore, the decrease in richness and diversity in mandarin fish fed the AF diet could likely contribute to a compromised health status compared to those in the NF group, as evidenced by the mild intestinal damage and immune suppression in previous studies ( Li et al, 2023 ).…”

Section: Discussionmentioning

confidence: 90%

“…In addition, supplementing carbohydrates or plant-derived proteins to the diet can reshape the gut microbiota of animals ( Li et al, 2016 ), and a high dietary carbohydrate may also reduce the microbial diversity in mandarin fish ( Zhang et al, 2020 ). Moreover, our previous publication has shown that slight damage was found in the intestine of mandarin fish fed the AF diet ( Li et al, 2023 ). Taken together, the inclusion of plant-derived proteins or carbohydrates in the AF diets led to a decrease in both the gut microbial diversity and richness, which may be related to the unexpected health status of the mandarin fish.…”

Section: Discussionmentioning

confidence: 96%

“…The present study was conducted in a pond system at the Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences (Guangzhou, China). The animal intervention procedure has been detailed in previous research by Li et al (2023) in details. In brief, domesticated mandarin fish were acquired from YuShun Fisheries Company (Qingyuan, China).…”

Section: Methodsmentioning

confidence: 99%

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Modulation of the gut microbiota by processed food and natural food: evidence from the Siniperca chuatsi microbiome

Li,

Niu,

Pan

et al. 2024

PeerJ

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Habitual dietary changes have the potential to induce alterations in the host’s gut microbiota. Mandarin fish (Siniperca chuatsi), an aquatic vertebrate species with distinct feeding habits, were fed with natural feeds (NF) and artificial feeds (AF) to simulate the effects of natural and processed food consumption on host gut microbiota assemblages. The results showed that the alpha diversity index was reduced in the AF diet treatment, as lower abundance and diversity of the gut microbiota were observed, which could be attributed to the colonized microorganisms of the diet itself and the incorporation of plant-derived proteins or carbohydrates. The β-diversity analysis indicated that the two dietary treatments were associated with distinct bacterial communities. The AF diet had a significantly higher abundance of Bacteroidota and a lower abundance of Actinomycetota, Acidobacteriota, and Chloroflexota compared to the NF group. In addition, Bacteroidota was the biomarker in the gut of mandarin fish from the AF treatment, while Acidobacteriota was distinguished in the NF treatments. Additionally, the increased abundance of Bacteroidota in the AF diet group contributed to the improved fermentation and nutrient assimilation, as supported by the metabolic functional prediction and transcriptome verification. Overall, the present work used the mandarin fish as a vertebrate model to uncover the effects of habitual dietary changes on the evolution of the host microbiota, which may provide potential insights for the substitution of natural foods by processed foods in mammals.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 96%

Section: Methodsmentioning

confidence: 99%

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Modulation of the gut microbiota by processed food and natural food: evidence from the Siniperca chuatsi microbiome

Li,

Niu,

Pan

et al. 2024

PeerJ

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“…The mandarin fish (Siniperca chuatsi) is a high-economic freshwater species with unique feeding habitats. To date, mandarin fish have been reported to be able to consume artificially formulated diets well after domestication [26,27]. As a typical carnivorous species, mandarin fish had a limited capacity to utilize dietary carbohydrates, making them a valuable model for investigating carbohydrate utilization in teleost fishes.…”

Section: Introductionmentioning

confidence: 99%

Dietary Betaine Attenuates High-Carbohydrate-Diet-Induced Oxidative Stress, Endoplasmic Reticulum Stress, and Apoptosis in Mandarin Fish (Siniperca chuatsi)

Li,

Zeng,

Zheng

et al. 2023

Antioxidants

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To investigate the impact of betaine on high-carbohydrate-diet-induced oxidative stress and endoplasmic reticulum (ER) stress, mandarin fish (Siniperca chuatsi) (23.73 ± 0.05 g) were fed with control (NC), betaine (BET), high carbohydrate (HC), and high carbohydrate + betaine (HC + BET) diets for 8 weeks. The results showed that betaine significantly promoted the growth of mandarin fish irrespective of the dietary carbohydrate levels. The HC diet induced oxidative stress, as evidenced by significantly elevated MDA levels. The HC diet significantly stimulated the mRNA levels of genes involved in ER stress (ire1, perk, atf6, xbp1, eif2α, atf4, chop), autophagy (ulk1, becn1, lc3b), and apoptosis (bax). However, betaine mitigated HC-diet-induced oxidative stress by modulating antioxidant enzymes and alleviated ER stress by regulating the mRNA of genes in the PERK-eIF2a-ATF4 pathway. Additionally, betaine significantly reduced the mRNA levels of becn1 and bax, along with the apoptosis rate, indicating a mitigating effect on autophagy and apoptosis. Overall, dietary betaine improved growth, attenuated HC-diet-induced oxidative stress and ER stress, and ultimately alleviated apoptosis in mandarin fish. These findings provide evidence for the use of betaine in aquafeeds to counter disruptive effects due to diets containing high carbohydrate levels.

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Evaluation of feed types based on growth performance, survival, hematology, and resistance in celebes rainbow (Marosantherina ladigesi)

Amrullah,

Wahidah,

Khatimah

et al. 2023

Fish Aquat Sci

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Celebes rainbow (Marosantherina ladigesi) is one of Indonesia's exported ornamental fish commodities, but the exploitation of this fish only relies on wild catches. The rise of unlimited fishing, especially those using poison, has changed the aquatic environment, threatening sustainability and causing fish extinction. This study aimed to evaluate the effectiveness of several types of feed in improving the absolute growth rate (AGR), specific growth rate (SGR), survival rate (SR), feed conversion ratio (FCR), feed efficiency (FE), hematology, and immune response of Celebes rainbow. The fish used in this study were male ornamental Celebes rainbow (M. ladigesi) weighing 1.32 ± 0.21 g/ind, reared in 54 L-aquariums at a stocking density of 30 individuals/aquarium for six weeks. The fish were fed according to the test diet consisting of live Tubifex sp worms, dry Tubifex sp worms, Spirulina platensis, and crumble pellets. The parameters observed were AGR, SGR, SR, FCR, FE, hematology, intestinal histology, liver histology, and a challenge test with the pathogenic bacteria Aeromonas hydrophila. The results showed that fish-fed live Tubifex sp worms had better AGR, SGR, SR, FCR, FE, hematology, and disease resistance compared to all other treatments. These results indicate that live Tubifex sp worms are the best feed for rearing Celebes rainbow.

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Integrated miRNA-mRNA analysis reveals the molecular mechanism in mandarin fish (Siniperca chuatsi) in response to fresh baits and artificial diets feeding

Cited by 3 publications

References 43 publications

Modulation of the gut microbiota by processed food and natural food: evidence from the Siniperca chuatsi microbiome

Modulation of the gut microbiota by processed food and natural food: evidence from the Siniperca chuatsi microbiome

Dietary Betaine Attenuates High-Carbohydrate-Diet-Induced Oxidative Stress, Endoplasmic Reticulum Stress, and Apoptosis in Mandarin Fish (Siniperca chuatsi)

Evaluation of feed types based on growth performance, survival, hematology, and resistance in celebes rainbow (Marosantherina ladigesi)

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