Integrated miRNA and transcriptome profiling to explore the molecular mechanism of Spodoptera frugiperda larval midgut in response to azadirachtin exposure

Shu, Benshui; Lin, Yanzheng; Qian, Guozhao; Cai, Xueming; Liu, Luyang; Lin, Jintian

doi:10.1016/j.pestbp.2022.105192

Cited by 11 publications

(2 citation statements)

References 56 publications

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“…The detoxification tissue, fat body, could be damaged by azadirachtin, resulting in structural damages in FAW . The semblable effect was also reported on the midgut in the larval of Bactrocera dorsalis and Spodoptera litura with azadirachtin exposure, ,, which could cause apoptosis and shedding of tissue cells, resulting in thinning of the intestinal wall and destruction of the intestinal tissue structure and further affecting the digestion and absorption function of the intestine. Besides, azadirachtin could suppress the lipid transportation to the ovary of Bombyx mori and cause short lifespan, ovarian abnormalities, lower fecundity, and compound eye abnormalities in Drosophila melanogaster .…”

Section: Introductionmentioning

confidence: 96%

Azadirachtin Inhibits Nuclear Receptor HR3 in the Prothoracic Gland to Block Larval Ecdysis in the Fall Armyworm, Spodoptera frugiperda

Fan,

Wu,

Liu

et al. 2023

J. Agric. Food Chem.

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Azadirachtin has been used to control agricultural pests for a long time; however, the molecular mechanism of azadirachtin on lepidopterans is still not clear. In this study, the fourth instar larvae of fall armyworm were fed with azadirachtin, and then the ecdysis was blocked in the fourth instar larval stage (L4). The prothoracic glands (PGs) of the treated larvae were dissected for RNA sequencing to determine the effect of azadirachtin on ecdysis inhibition. Interestingly, one of the PG-enriched genes, the nuclear hormone receptor 3 (HR3), was decreased after azadirachtin treatment, which plays a critical role in the 20-hydroxyecdysone action during ecdysis. To deepen the understanding of azadirachtin on ecdysis, the HR3 was knocked out by using the CRISPR/Cas9 system, while the HR3 mutants displayed embryonic lethal phenotype; thus, the stage-specific function of HR3 during larval molting was not enabled to unfold. Hence, the siRNA was injected into the 24 h L4 larvae to knock down HR3. After 96 h, the injected larvae were blocked in the old cuticle during ecdysis which is consistent with the azadirachtin-treated larvae. Taken together, we envisioned that the inhibition of ecdysis in the fall armyworm after the azadirachtin treatment is due to an interference with the expression of HR3 in PG, resulting in larval mortality. The results in this study specified the understanding of azadirachtin on insect ecdysis and the function of HR3 in lepidopteran in vivo.

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Section: Introductionmentioning

confidence: 96%

Azadirachtin Inhibits Nuclear Receptor HR3 in the Prothoracic Gland to Block Larval Ecdysis in the Fall Armyworm, Spodoptera frugiperda

Fan,

Wu,

Liu

et al. 2023

J. Agric. Food Chem.

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“… Zhu et al (2021b) identified a set of insecticide-responsive cuticle protein (CP) genes by determining the response of CP genes of S. frugiperda under different insecticide treatments. Another study obtained 13 and 178 insecticide-responsive miRNA–mRNA pairs through the midgut RNA-sequencing (RNA-seq) analysis of azoxystrobin-treated S. frugiperda larvae ( Shu et al 2022 ). New insecticides often achieve good results, and the study of the transcriptional response of S. frugiperda after the treatment with new insecticides contributes to the understanding of the mechanism.…”

Section: Introductionmentioning

confidence: 99%

Response patterns of lncRNAs of theSpodoptera frugiperda(Lepidoptera: Noctuidae) larvae under 23 pesticide treatments

Wang

Xiao

et al. 2023

Journal of Insect Science

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The response of Spodoptera frugiperda genes toward insecticides is crucial for guiding insecticide use. The regulation of the S. frugiperda genes via long noncoding RNAs (lncRNAs) under insecticide treatment should be investigated. In this study, 452 differentially expressed lncRNAs were identified by analyzing RNA-sequencing data of S. frugiperda under 23 pesticide treatments. We found 59 and 43 differentially expressed lncRNAs that could regulate detoxification-related cytochrome P450 and UDP-glucuronosyltransferase genes, respectively. Furthermore, the target genes of differentially expressed lncRNAs were enriched in Pfam, including chitin bind 4 and gene ontology terms such as structural constituent of the cuticle, revealing their potential mechanism of action on the growth inhibition of S. frugiperda larvae. Insecticide-specific expression of lncRNAs highlights the properties and commonalities of different insecticide-induced lncRNA regulatory mechanisms. To conclude, the results of this study provide new insights and perspectives on the use of 23 insecticides via lncRNA regulation of mRNAs.

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Tailored midgut gene expression in Spodoptera litura (Lepidoptera: Noctuidae) feeding on Zea mays indicates a tug of war

Singh,

Kumar,

Yadav

et al. 2024

Arthropod-Plant Interactions

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Integrated miRNA and transcriptome profiling to explore the molecular mechanism of Spodoptera frugiperda larval midgut in response to azadirachtin exposure

Cited by 11 publications

References 56 publications

Azadirachtin Inhibits Nuclear Receptor HR3 in the Prothoracic Gland to Block Larval Ecdysis in the Fall Armyworm, Spodoptera frugiperda

Azadirachtin Inhibits Nuclear Receptor HR3 in the Prothoracic Gland to Block Larval Ecdysis in the Fall Armyworm, Spodoptera frugiperda

Response patterns of lncRNAs of theSpodoptera frugiperda(Lepidoptera: Noctuidae) larvae under 23 pesticide treatments

Tailored midgut gene expression in Spodoptera litura (Lepidoptera: Noctuidae) feeding on Zea mays indicates a tug of war

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