Integrated microfluidic systems for molecular diagnostics: A universal electrode platform for rapid diagnosis of urinary tract infections

Sin, Mandy L.Y.; Gau, Vincent; Liao, Joseph C.; Wong, Pak Kin

doi:10.1109/mnano.2012.2237331

Cited by 9 publications

(3 citation statements)

References 20 publications

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“…Incorporation of such mechanisms, however, greatly reduces the portability of the device for point-of-care applications. 16, 17 This represents a fundamental hurdle for implementing μ-TAS in point-of-care diagnostic applications. Electrokinetic pumps, which do not involve any mechanical moving parts, can be fabricated easily and resolve the need of external driving mechanisms necessary for microfluidic system integration.…”

Section: Microfluidic Operationsmentioning

confidence: 99%

“…By properly applying knowledge of the phenomenon, ACEF could be an effective AC electrokinetic technique for system integration, leading toward point-of-care diagnostics because of its effectiveness at physiological conductivity. 17 The technique is further applicable to various types of samples and applications. Since molecular advection and molecular binding efficiency are the fundamental barriers that are commonly observed in various biomedical assays, ACEF enhancement has a great potential to benefit other sensing platforms found in clinical and biochemical applications.…”

Section: Future Directionmentioning

confidence: 99%

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AC Electrokinetics of Physiological Fluids for Biomedical Applications

Liu

Lamanda

et al. 2015

SLAS Technology

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AC electrokinetics is a collection of processes for manipulating bulk fluid mass and embedded objects with AC electric fields. The ability of AC electrokinetics to implement the major microfluidic operations, such as pumping, mixing, concentration and separation, makes it possible to develop integrated systems for clinical diagnostics in non-traditional healthcare settings. The high conductivity of physiological fluids presents new challenges and opportunities for AC electrokinetics based diagnostic systems. In this review, AC electrokinetic phenomena in conductive physiological fluids are described followed by a review of the basic microfluidic operations and the recent biomedical applications of AC electrokinetics. The future prospects of AC electrokinetics for clinical diagnostics are presented.

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Section: Microfluidic Operationsmentioning

confidence: 99%

Section: Future Directionmentioning

confidence: 99%

AC Electrokinetics of Physiological Fluids for Biomedical Applications

Liu

Lamanda

et al. 2015

SLAS Technology

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“…Rapid and more accurate diagnostic technologies/tools are key issues in the identification of pathogenic microorganisms including prokaryotic bacteria, eukaryotic fungi, viruses, parasites, prions and protozoa for clinical diagnostic purposes (Na et al, 2018). Microfluidic systems are automated manipulation devices containing microchannels allowing liquid flow (10–100μm), and several other components including micro-pumps, micro scale inlet valves and miniaturized outlet drains combined with different apparatus for analysis of the contents (Park et al., 2011; Sin et al, 2013). Microfluidic chips have been deployed to track pathogens, identify food-based microorganisms, as well as to discover new antimicrobial agents (Lei, 2012).…”

Section: Introductionmentioning

confidence: 99%

Point-of-care microfluidic devices for pathogen detection

Nasseri

Soleimani

Rabiee

et al. 2018

Biosensors and Bioelectronics

312

166

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The rapid diagnosis of pathogens is crucial in the early stages of treatment of diseases where the choice of the correct drug can be critical. Although conventional cell culture-based techniques have been widely utilized in clinical applications, newly introduced optical-based, microfluidic chips are becoming attractive. The advantages of the novel methods compared to the conventional techniques comprise more rapid diagnosis, lower consumption of patient sample and valuable reagents, easy application, and high reproducibility in the detection of pathogens. The miniaturized channels used in microfluidic systems simulate interactions between cells and reagents in microchannel structures, and evaluate the interactions between biological moieties to enable diagnosis of microorganisms. The overarching goal of this review is to provide a summary of the development of microfluidic biochips and to comprehensively discuss different applications of microfluidic biochips in the detection of pathogens. New types of microfluidic systems and novel techniques for viral pathogen detection (e.g. HIV, HVB, ZIKV) are covered. Next generation techniques relying on high sensitivity, specificity, lower consumption of precious reagents, suggest that rapid generation of results can be achieved via optical based detection of bacterial cells. The introduction of smartphones to replace microscope based observation has substantially improved cell detection, and allows facile data processing and transfer for presentation purposes.

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