Integrated microfluidic devices for point-of-care detection of bio-analytes and disease

Behera, Prateechee Padma; Kumar, Natish; Kumari, Monika; Kumar, Sumit; Mondal, Pranab Kumar; Arun, Ravi Kumar

doi:10.1039/d3sd00170a

Cited by 11 publications

(3 citation statements)

References 185 publications

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“…As precision medicine enters the multi-omics era, technology also needs to evolve [118]. Therefore, strengthening the healthcare system is the most ideal way to keep the population healthy and maintain a productive workforce [119]. As such, we propose a miniaturized point-of-care system based on a two-dimensional capture-separation system capable of analyzing multiple biomarkers that can be identified and characterized to avoid falsepositive and false-negative diagnostic results.…”

Section: Future Evolution and Benefits Of A Point-of-care Platform Fo...mentioning

confidence: 99%

Human Sputum Proteomics: Advancing Non-Invasive Diagnosis of Respiratory Diseases with Enhanced Biomarker Analysis Methods

Guzman,

Guzman

2024

IJTM

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Many ailments can be diagnosed while they are asymptomatic, meaning that the patient has no signs or symptoms of a progressing disease. If caught in their initial stage of formation, these disorders can be effectively treated, leading to successful outcomes; curative therapies can halt illnesses from advancing, thus improving the quality of life and long-term survival of the patient. Still, cutting-edge upgrades in precision technologies are necessary for early, reliable, affordable, and rapid disease detection, but also vital for the well-being of people and the future of global public health. The emerging role and utility of non-invasive and repeatable diagnostic test approaches for the detection of health conditions have been exemplified by liquid biopsies based on genomic biomarkers. As such, biological fluids permit any measurable molecular indicator or signature (e.g., proteins) to provide valuable information on an individual’s wellness and/or disease. Among the bodily secretions used for non-invasive diagnostics is sputum, a complex viscous gel-like biopolymeric network that has gained growing recognition as a rich source of biomarkers of airway infections and pulmonary diseases, and serves as a determinant to reveal other illnesses. As per the World Health Organization, the burden of respiratory conditions is exacerbated by factors ranging from considerable subjection to air pollution and occupational contaminants to tobacco smoking and second-hand smoke, in addition to poor socio-economic status. Due to the likely increase in these determinants, respiratory tract ailments are on the rise, affecting the health of many individuals, in addition to putting stress on healthcare facilities and services worldwide. The aim of this study was to perform a narrative review of sputum constituents with an emphasis on proteins and glycoproteins assessed as possible biomarkers of lung and other organ diseases. A search was conducted using mucus, sputum proteomics, sputum biomarkers, and point-of-care testing as keywords employing Google, PubMed (MEDLINE), and Web of Science, selecting the most referenced and related papers of the last decade. We, therefore, highlight the need to use expectorated or induced sputum specimens as a routine sample source for testing valuable protein biomarkers to diagnose these chronic disorders, predict inflammation and disease progression, as well as monitor the effectiveness of treatments. Further, we discuss the urgent need for fast and reliable point-of-care methods to detect and quantify crucial protein biomarkers in sputum specimens, and the limitations faced when dealing with their complex matrices.

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Section: Future Evolution and Benefits Of A Point-of-care Platform Fo...mentioning

confidence: 99%

Human Sputum Proteomics: Advancing Non-Invasive Diagnosis of Respiratory Diseases with Enhanced Biomarker Analysis Methods

Guzman,

Guzman

2024

IJTM

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“…The coexistence of multiple biomarkers in a complex microenvironment significantly influences the sensing performance of the sensor. In such an environment, employing a single fluorescence probe for multiplexed detection not only offers simplified and cost-effective methods but also improves the accuracy and reliability of the probe as a diagnostic tool. , Nowadays, carbon dots, with their exceptional photoluminescence properties and biocompatibility, have emerged as an excellent choice for the construction of multifunctional fluorescent probes. Although the simultaneous detection of various environmental analytes using a carbon dot fluorescence probe has been extensively reported, − the exploration of multiplexed detection of biomarkers using a single probe is still in its early stages.…”

Section: Introductionmentioning

confidence: 99%

Zn-CD@Eu Ratiometric Fluorescent Probe for the Detection of Dipicolinic Acid, Uric Acid, and Ex Vivo Uric Acid Imaging

Mohapatro,

Mishra,

Mishra

et al. 2024

Anal. Chem.

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Development of reliable methods for the detection of potential biomarkers is of the utmost importance for an early diagnosis of critical diseases and disorders. In this study, a novel lanthanide-functionalized carbon dot-based fluorescent probe Zn-CD@Eu is reported for the ratiometric detection of dipicolinic acid (DPA) and uric acid (UA). The Zn-CD@Eu nanoprobe was obtained from a simple room-temperature reaction of zinc-doped carbon dots (Zn-CD) and the EDTA–Eu lanthanide complex. Under optimal conditions, a good linear response was obtained for DPA in two concentration ranges of 0−55 and 55−100 μM with a limit of detection of 0.53 and 2.2 μM respectively, which is significantly below the infectious dosage of anthrax (∼55 μM). Furthermore, the Zn-CD@Eu/DPA system was employed for the detection of UA with a detection limit of 0.36 μM in the linear range of 0–100 μM. The fluorescent probe was successfully implemented for determining DPA and UA in human blood serum, sweat, and natural water bodies with considerable recovery rates. In addition, the potential of the nanoprobe for ex vivo visualization of UA was demonstrated in fruit fly (Drosophila melanogaster) as a model organism.

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“…In the past decade, microfluidic paper-based analytical devices (μPADs) have been demonstrated for quantitative analysis in different applications, such as in the medical field, healthcare, and environmental monitoring. 18–20 μPADs are low-cost, easy-to-fabricate miniaturized systems that are suitable for sample-to-answer applications. 21–24 The colorimetric detection of Cys using microfluidics-based platforms is a potential analytical method.…”

Section: Introductionmentioning

confidence: 99%

A paper-based point-of-care device for the detection of cysteine using gold nanoparticles from whole blood

Kumari,

Kumar,

Kumar

et al. 2024

Anal. Methods

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Integrated microfluidic devices for point-of-care detection of bio-analytes and disease

Abstract: Micro- and nanofluidics have enabled the possibility of miniaturized analytical systems, which are commonly known as lab-on-a-chip (LOC) devices.

Cited by 11 publications

References 185 publications

Human Sputum Proteomics: Advancing Non-Invasive Diagnosis of Respiratory Diseases with Enhanced Biomarker Analysis Methods

Human Sputum Proteomics: Advancing Non-Invasive Diagnosis of Respiratory Diseases with Enhanced Biomarker Analysis Methods

Zn-CD@Eu Ratiometric Fluorescent Probe for the Detection of Dipicolinic Acid, Uric Acid, and Ex Vivo Uric Acid Imaging

A paper-based point-of-care device for the detection of cysteine using gold nanoparticles from whole blood

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