Integrated microfluidic device for the separation, decomposition and detection of low molecular weight S-nitrosothiols

Duarte-Junior, Gerson F.; Ismail, Abdulghani; Griveau, Sophie; d’Orlyé, Fanny; Silva, José Alberto Fracassi da; Coltro, Wendell K. T.; Bédioui, Féthi; Varenne, Anne

doi:10.1039/c8an00757h

Cited by 8 publications

(7 citation statements)

References 32 publications

(28 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For facilitating the photolysis of nitrosothiol groups and realizing the amperometric detection of nitrite, the authors reported a simple degradation through UV-LED and an analysis with a procedure time shorter than 2 min and LOD value of 73 nmol L –1 . As above-discussed, the proposed modified PES provides suitable detectability for CySNO and the estimated LOD is better or similar when compared to low-cost analytical tools. − Also, the herein reported linear concentration range (10–125 μmol L –1 ) can be useful to clinical application that involved Parkinson’s disease …”

Section: Resultsmentioning

confidence: 64%

See 1 more Smart Citation

Recycling 3D Printed Residues for the Development of Disposable Paper-Based Electrochemical Sensors

Silva-Neto

Duarte-Junior

Rocha

et al. 2023

ACS Appl. Mater. Interfaces

Self Cite

View full text Add to dashboard Cite

Here, we propose a recyclable approach using acrylonitrile-butadiene-styrene (ABS) residues from additive manufacturing in combination with low-cost and accessible graphite flakes as a novel and potential mixture for creating a conductive paste. The graphite particles were successfully incorporated in the recycled thermoplastic composite when solubilized with acetone and the mixture demonstrated greater adherence to different substrates, among which cellulose-based material made possible the construction of a paper-based electrochemical sensor (PES). The morphological, structural, and electrochemical characterizations of the recycled electrode material were demonstrated to be similar to those of the traditional carbon-based surfaces. Faradaic responses based on redox probe activity ([Fe(CN)6]3‑/4–) exhibited well-defined peak currents and diffusional mass transfer as a quasi-reversible system (96 ± 5 mV) with a fast heterogeneous rate constant value of 2 × 10–3 cm s–1. To improve the electrode electrochemical properties, both the PES and the classical 3D-printed electrode surfaces were modified with a combination of multiwalled carbon nanotubes (MWCNTs), graphene oxide (GO), and copper. Both electrode surfaces demonstrated the suitable oxidation of nitrite at 0.6 and 0.5 V vs Ag, respectively. The calculated analytical sensitivities for PES and 3D-printed electrodes were 0.005 and 0.002 μA/(μmol L–1), respectively. The proposed PES was applied for the indirect amperometric analysis of S-nitroso-cysteine (CysNO) in serum samples via nitrite quantitation, demonstrating a limit of detection of 4.1 μmol L–1, with statistically similar values when compared to quantitative analysis of the same samples by spectrophotometry (paired t test, 95% confidence limit). The evaluated electroanalytical approach exhibited linear behavior for nitrite in the concentration range between 10 and 125 μmol L–1, which is suitable for realizing clinical diagnosis involving Parkinson’s disease, for example. This proof of concept shows the great promise of this recyclable strategy combining ABS residues and conductive particles in the context of green chemical protocols for constructing disposable sensors.

show abstract

Section: Resultsmentioning

confidence: 64%

“…The achieved LOD was compared to other values found in recent published reports. Duarte-Junior et al 65 proposed a microfluidic device with integrated electrochemical detector for separating and detecting RSNOs, after a decomposition to nitrite based on mercury with LODs of 20 μmol L −1 . Ismail et al.…”

Section: Recycled Pes For Cysno Sensingmentioning

confidence: 99%

Recycling 3D Printed Residues for the Development of Disposable Paper-Based Electrochemical Sensors

Silva-Neto

Duarte-Junior

Rocha

et al. 2023

ACS Appl. Mater. Interfaces

Self Cite

View full text Add to dashboard Cite

show abstract

“…Therefore, a buffer solution composed of lactic acid and histidine was prepared at different ratios to investigate the pH effect on nitrite determination. Th analyses were carried out in an acidic medium to favor nitrite oxidation [ 65 , 66 , 67 , 68 ]. For this purpose, the lactic acid concentration was fixed at 30 mmol L −1 and the histidine concentration ranged from 5 to 25 mmol L −1 (5 mmol L −1 increments), providing solutions with pH at 3.2, 3.6, 3.8, 4.1, and 4.5, respectively (a–e) ( Figure 2 A).…”

Section: Resultsmentioning

confidence: 99%

Nitrite Determination in Environmental Water Samples Using Microchip Electrophoresis Coupled with Amperometric Detection

et al. 2022

View full text Add to dashboard Cite

Nitrite is considered an important target analyte for environmental monitoring. In water resources, nitrite is the result of the nitrogen cycle and the leaching processes of pesticides based on nitrogenous compounds. A high concentration of nitrite can be associated with intoxication processes and metabolic disorders in humans. The present study describes the development of a portable analytical methodology based on microchip electrophoresis coupled with amperometric detection for the determination of nitrite in environmental water samples. Electrophoretic and detection conditions were optimized, and the best separations were achieved within 60 s by employing a mixture of 30 mmol L−1 lactic acid and 15 mmol L−1 histidine (pH = 3.8) as a running buffer applying 0.7 V to the working electrode (versus Pt) for amperometric measurements. The developed methodology revealed a satisfactory linear behavior in the concentration range between 20 and 80 μmolL−1 (R2 = 0.999) with a limit of detection of 1.3 μmolL−1. The nitrite concentration was determined in five water samples and the achieved values ranged from (28.7 ± 1.6) to (67.1 ± 0.5) µmol L−1. The data showed that using the proposed methodology revealed satisfactory recovery values (83.5–103.8%) and is in good agreement with the reference technique. Due to its low sample consumption, portability potential, high analytical frequency, and instrumental simplicity, the developed methodology may be considered a promising strategy to monitor and quantitatively determine nitrite in environmental samples.

show abstract

“…More recently, Duarte et al [124] reported a SU-8/Pyrex system with integrated Pt electrodes in an end-channel configuration, for the separation, decomposition (under metal…”

Section: Application In Analysis Of Biological Samplesmentioning

confidence: 99%

Microchip electrophoresis and electrochemical detection: A review on a growing synergistic implementation

Costa

Griveau

d’Orlyé

et al. 2021

Electrochimica Acta

Self Cite

View full text Add to dashboard Cite

Integrated microfluidic device for the separation, decomposition and detection of low molecular weight S-nitrosothiols

Abstract: S-nitrosothiols (RSNOs) are electrokinetically separated and the nitrite generated by decomposition with mercury(ii) is detected by amperometry, in an integrated microdevice.

Cited by 8 publications

References 32 publications

Recycling 3D Printed Residues for the Development of Disposable Paper-Based Electrochemical Sensors

Recycling 3D Printed Residues for the Development of Disposable Paper-Based Electrochemical Sensors

Nitrite Determination in Environmental Water Samples Using Microchip Electrophoresis Coupled with Amperometric Detection

Microchip electrophoresis and electrochemical detection: A review on a growing synergistic implementation

Contact Info

Product

Resources

About