Integrated metabolomics and network pharmacology strategy for ascertaining the quality marker of flavonoids for Sophora flavescens

Chen, Lei; Huang, Xiaobin; Wang, Huan; Shao, Jing; Luo, Yun; Zhao, Kairui; Liu, Yi; Wang, Shumei

doi:10.1016/j.jpba.2020.113297

Cited by 18 publications

(8 citation statements)

References 38 publications

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“…It is widely believed that young S. flavescens plants do not produce as much matrine and oxymatrine as older plants, although we could not find any English scientific literature that documents the actual timing of increased production. A recent paper on the flavonoids produced by S. flavescens showed statistically significant differences from year 1 to year 6 ( Chen et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Analysis of Genetic Diversity in the Traditional Chinese Medicine Plant ‘Kushen’ (Sophora flavescens Ait.)

et al. 2021

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Kushen root, from the woody legume Sophora flavescens, is a traditional Chinese medicine that is a key ingredient in several promising cancer treatments. This activity is attributed in part to two quinolizidine alkaloids (QAs), oxymatrine and matrine, that have a variety of therapeutic activities in vitro. Genetic selection is needed to adapt S. flavescens for cultivation and to improve productivity and product quality. Genetic diversity of S. flavescens was investigated using genotyping-by-sequencing (GBS) on 85 plants grown from seeds collected from 9 provinces of China. DArTSeq provided over 10,000 single nucleotide polymorphism (SNP) markers, 1636 of which were used in phylogenetic analysis to reveal clear regional differences for S. flavescens. One accession from each region was selected for PCR-sequencing to identify gene-specific SNPs in the first two QA pathway genes, lysine decarboxylase (LDC) and copper amine oxidase (CAO). To obtain SfCAO sequence for primer design we used a targeted transcript capture and assembly strategy using publicly available RNA sequencing data. Partial gene sequence analysis of SfCAO revealed two recently duplicated genes, SfCAO1 and SfCAO2, in contrast to the single gene found in the QA-producing legume Lupinus angustifolius. We demonstrate high efficiency converting SNPs to Kompetitive Allele Specific PCR (KASP) markers developing 27 new KASP markers, 17 from DArTSeq data, 7 for SfLDC, and 3 for SfCAO1. To complement this genetic diversity analysis a field trial site has been established in South Australia, providing access to diverse S. flavescens material for morphological, transcriptomic, and QA metabolite analysis. Analysis of dissected flower buds revealed that anthesis occurs before buds fully open suggesting a potential for S. flavescens to be an inbreeding species, however this is not supported by the relatively high level of heterozygosity observed. Two plants from the field trial site were analysed by quantitative real-time PCR and levels of matrine and oxymatrine were assessed in a variety of tissues. We are now in a strong position to select diverse plants for crosses to accelerate the process of genetic selection needed to adapt kushen to cultivation and improve productivity and product quality.

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Section: Discussionmentioning

confidence: 99%

Analysis of Genetic Diversity in the Traditional Chinese Medicine Plant ‘Kushen’ (Sophora flavescens Ait.)

et al. 2021

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“…Chen et al . developed a novel integrated strategy, combining metabolomics and network pharmacology, to explore the six potential quality markers of Sophora flavescens based on the network of “component‐target” 15 …”

Section: Introductionmentioning

confidence: 99%

“…Chen et al developed a novel integrated strategy, combining metabolomics and network pharmacology, to explore the six potential quality markers of Sophora flavescens based on the network of "component-target". 15 Liao's study adopted an integrated approach using chemical analysis and network pharmacology to discover the quality (Q) marker for lipid metabolism of Alisma orientale. Network pharmacology revealed the unique relationship between Q-markers and their pharmacological action 16 and is therefore a powerful tool used in the discovery of Q-markers.…”

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confidence: 99%

An integrated chemical analysis and network pharmacology approach to identify quality markers of Actinidia eriantha Benth radix on gastric cancer

Xiang

Chen

Qiu

2022

Phytochemical Analysis

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Introduction Actinidia eriantha Benth radix (AEBR) is one of the most commonly used medicines by the She people in China, used primarily for the treatment of tumours of the digestive tract. There is currently limited to no data on the quality control of AEBR. Objectives The aim of this study was to identify quality markers of AEBR. Material and Methods An ultra‐performance lquid chromatography quadrupole time‐of‐flight mass spectrometry (UPLC‐QTOF‐MS) method was used to identify and analyse the components of AEBR from water extracts. An ultra‐performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method was also established for the simultaneous determination of 13 active components in the water extracts. The network pharmacology method was used to screen for quality markers of AEBR in gastric cancer. Results This study tentatively identified 199 chemical constituents and isomers, including 67 pentacyclic triterpenoids, 20 flavonoids, 39 phenolic acids, 18 coumarins, and other compounds. The 13 active components in the water extracts were successfully determined using a validated UPLC–MS/MS method. Based on the network pharmacology method, four compounds were selected as quality markers of AEBR. Conclusion This study provides an important reference for the quality control of AEBR. Chemical analysis combined with network pharmacology provides an effective strategy for the discovery of quality markers in traditional Chinese/herb medicine.

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“…Kurarinone containing propolis produced significant in vivo anti-inflammatory potential ( Sahlan et al, 2019 ). Chen et al (2020) studied the mechanistic efficacy of S. flavescens ethanolic extract in dextran sodium sulfate-induced ulcerative colitis rats. Ulcerative colitis (UC) is an immunological disease; if not treated, it may lead to colon cancer.…”

Section: Introductionmentioning

confidence: 99%

“…The study concluded that the compounds such as kurarinone can alter the UT-related pathophysiological conditions (colon length and healthiness of colon tissue, bleeding, etc.) in an experimental rat model ( Chen et al, 2020 ). Kim et al (2013) studied the immune response inhibitory potential of kurarinone in psoriasis-like skin disease and contact dermatitis experimental chronic inflammatory skin models.…”

Section: Introductionmentioning

confidence: 99%

Five-Decade Update on Chemopreventive and Other Pharmacological Potential of Kurarinone: a Natural Flavanone

et al. 2021

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In the present article we present an update on the role of chemoprevention and other pharmacological activities reported on kurarinone, a natural flavanone (from 1970 to 2021). To the best of our knowledge this is the first and exhaustive review of kurarinone. The literature was obtained from different search engine platforms including PubMed. Kurarinone possesses anticancer potential against cervical, lung (non-small and small), hepatic, esophageal, breast, gastric, cervical, and prostate cancer cells. In vivo anticancer potential of kurarinone has been extensively studied in lungs (non-small and small) using experimental xenograft models. In in vitro anticancer studies, kurarinone showed IC50 in the range of 2–62 µM while in vivo efficacy was studied in the range of 20–500 mg/kg body weight of the experimental organism. The phytochemical showed higher selectivity toward cancer cells in comparison to respective normal cells. kurarinone inhibits cell cycle progression in G2/M and Sub-G1 phase in a cancer-specific context. It induces apoptosis in cancer cells by modulating molecular players involved in apoptosis/anti-apoptotic processes such as NF-κB, caspase 3/8/9/12, Bcl2, Bcl-XL, etc. The phytochemical inhibits metastasis in cancer cells by modulating the protein expression of Vimentin, N-cadherin, E-cadherin, MMP2, MMP3, and MMP9. It produces a cytostatic effect by modulating p21, p27, Cyclin D1, and Cyclin A proteins in cancer cells. Kurarinone possesses stress-mediated anticancer activity and modulates STAT3 and Akt pathways. Besides, the literature showed that kurarinone possesses anti-inflammatory, anti-drug resistance, anti-microbial (fungal, yeast, bacteria, and Coronavirus), channel and transporter modulation, neuroprotection, and estrogenic activities as well as tyrosinase/diacylglycerol acyltransferase/glucosidase/aldose reductase/human carboxylesterases 2 inhibitory potential. Kurarinone also showed therapeutic potential in the clinical study. Further, we also discussed the isolation, bioavailability, metabolism, and toxicity of Kurarinone in experimental models.

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Integrated metabolomics and network pharmacology strategy for ascertaining the quality marker of flavonoids for Sophora flavescens

Cited by 18 publications

References 38 publications

Analysis of Genetic Diversity in the Traditional Chinese Medicine Plant ‘Kushen’ (Sophora flavescens Ait.)

Analysis of Genetic Diversity in the Traditional Chinese Medicine Plant ‘Kushen’ (Sophora flavescens Ait.)

An integrated chemical analysis and network pharmacology approach to identify quality markers of Actinidia eriantha Benth radix on gastric cancer

Five-Decade Update on Chemopreventive and Other Pharmacological Potential of Kurarinone: a Natural Flavanone

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