Integrated lipidomics and network pharmacology analysis of the protective effects and mechanism of Yuanzhi San on rats with cognitive impairment

Wang, Jiamin; Li, Jianli; Liu, Kaili; Wang, Shihui; SU, Qihui; Chen, Yangang; Wang, Yan

doi:10.1016/j.bmc.2022.116651

Cited by 7 publications

(5 citation statements)

References 61 publications

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“…Cognitive impairment seriously affects people’s physical and mental health and also causes a substantial economic burden [ 29 ]. This study showed that HRW can regulate the glycerophospholipid metabolism, which is the most abundant phospholipid in the body, and can activate the phospholipase A2 pathway to release arachidonic acid, thereby regulating the metabolism of glycerophospholipids and improve depression [ 30 ]. In addition, ascorbate and aldarate metabolism, pentose and gluconate interconversions are closely related to cognitive impairment [ 31 , 32 ].…”

Section: Discussionmentioning

confidence: 99%

Comprehensive brain tissue metabolomics and biological network technology to decipher the mechanism of hydrogen-rich water on Radiation-induced cognitive impairment in rats

Liu,

Liu

et al. 2023

BMC Mol and Cell Biol

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Background Hydrogen-rich water (HRW) has been shown to prevent cognitive impairment caused by ionizing radiation. This study aimed to investigate the pharmacological effects and mechanisms of HRW on ionizing radiation by coupling the brain metabolomics and biological target network methods. Methods and results HRW significantly improves the cognitive impairment in rats exposed to ionizing radiation. Based on metabolomics and biological network results, we identified 54 differential metabolites and 93 target genes. The KEGG pathway indicates that glutathione metabolism, ascorbic acid and aldehyde acid metabolism, pentose and glucuronic acid interconversion, and glycerophospholipid metabolism play important roles in ionizing radiation therapy. Conclusion Our study has systematically elucidated the molecular mechanism of HRW against ionizing radiation, which can be mediated by modulating targets, pathways and metabolite levels. This provides a new perspective for identifying the underlying pharmacological mechanism of HRW.

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Section: Discussionmentioning

confidence: 99%

Comprehensive brain tissue metabolomics and biological network technology to decipher the mechanism of hydrogen-rich water on Radiation-induced cognitive impairment in rats

Liu,

Liu

et al. 2023

BMC Mol and Cell Biol

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“…YZS was weighed based on the weight ratio of Codonopsis , Poria , Polygalae Radix , Acori Tatarinowii Rhizoma , and Coptidis Rhizoma , which were in the following mass ratio: 18 : 15 : 12 : 18 : 12 g. After crushing the above components, 10 mL/g of 60 % ethanol was added, followed by reflux extraction for 85 min. Then, the ethanol was recovered, and the mixture was concentrated until a dry extract was obtained [19,26] …”

Section: Methodsmentioning

confidence: 99%

“…Then, the ethanol was recovered, and the mixture was concentrated until a dry extract was obtained. [19,26] Methanol (batch number: 173433) was purchased from Thermo Fisher Scientific. Acetonitrile (batch number: 20AA10225M01) was purchased from Minobell Company.…”

Section: Methodsmentioning

confidence: 99%

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Integrated Lipidomics and Network Pharmacology Reveal Mechanism of Memory Impairment Improvement by Yuanzhi San

Zhao

Deng

et al. 2023

Chemistry & Biodiversity

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Memory impairment (MI) is caused by a variety of causes, endangering human health. Yuanzhi San (YZS) is a common prescription used for the treatment of MI, but its mechanism of action needs further exploration. The purpose of this study was to investigate this mechanism through lipidomics and network pharmacology. Sprague Dawley (SD) rats were divided randomly into the normal, model, and YZS groups. The rats were gavaged with aluminum chloride (200 mg/kg) and intraperitoneally injected with D-galactose (400 mg/kg) every day for 60 days, except for the normal group. From the 30 th day, YZS (13.34 g/kg) was gavaged once a day to the rats in the YZS group. Post-YZS treatment, ultra-high-performance liquid chromatography-mass spectrometry (UHPLC/MS) analysis was implemented to conduct a lipidomics study in the hippocampus of rats with memory impairment induced by aluminum chloride and D-galactose. Eight differential metabolites were identified between the normal group and the model group, whereas between the model group and the YZS group, 20 differential metabolites were established. Metabolic pathway analysis was performed on the aforementioned lipid metabolites, all of which were involved in sphingolipid and glycerophospholipid metabolism. Furthermore, serum pharmacochemistry analysis of YZS was carried out at the early stage of our research, which discovered 62 YZS prototype components. The results of the network pharmacology analysis showed that they were related to 1030 genes, and 451 disease genes were related to MI. There were 73 intersections between the YZS and MI targets. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that these targets were closely related to the sphingolipid metabolic, calcium signaling, and other pathways. The integrated approach of lipidomics and network pharmacology was then focused on four major targets, including PHK2, GBA, SPTLC1, and AChE, as well as their essential metabolites (glucosylceramide, N-acylsphingosine, phosphatidylserine, phosphatidylcholine, and phosphatidylcholine) and pathways (sphingolipid, glycerophospholipid, and arachidonic acid metabolism). The significant affinity of the primary target for YZS was confirmed by molecular docking. The obtained results revealed that the combination of lipidomics and network pharmacology could be used to determine the effect of YZS on the MI biological network and metabolic state, and evaluate the drug efficacy of YZS and its related mechanisms of action.

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“…A standard curve was established for the calculation of the content of Ca 2+ , CaM, and CaMKII in the mouse serum with a MULTISKANFC microplate reader (Thermo Fisher, USA). 12…”

Section: Methodsmentioning

confidence: 99%

Serum lipidomics reveal the mechanism of memory disorder improvement by Qifu decoction

Deng,

Zhao,

Wang

et al. 2023

Anal. Methods

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Integrated lipidomics and network pharmacology analysis of the protective effects and mechanism of Yuanzhi San on rats with cognitive impairment

Cited by 7 publications

References 61 publications

Comprehensive brain tissue metabolomics and biological network technology to decipher the mechanism of hydrogen-rich water on Radiation-induced cognitive impairment in rats

Comprehensive brain tissue metabolomics and biological network technology to decipher the mechanism of hydrogen-rich water on Radiation-induced cognitive impairment in rats

Integrated Lipidomics and Network Pharmacology Reveal Mechanism of Memory Impairment Improvement by Yuanzhi San

Serum lipidomics reveal the mechanism of memory disorder improvement by Qifu decoction

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