Integrated isotope and microbiome analysis indicates dominance of denitrification in N2O production after rewetting of drained fen peat

Masta, Mohit; Espenberg, Mikk; Gadegaonkar, Sharvari Sunil; Pärn, Jaan; Sepp, Holar; Kirsimäe, Kalle; Sgouridis, Fotis; Müller, Christoph; Mander, Ülo

doi:10.1007/s10533-022-00971-3

Cited by 13 publications

(10 citation statements)

References 63 publications

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“…Gammaproteobacteria as the predominant representing denitrifiers harboring both nir S and nos Z genes were found to be the most abundant denitrifying population in lakes, mangroves, estuaries, and marine sediments. , Thus, the dominant presence of Gammaproteobacteria in Lake Taihu indicated that denitrifiers indeed played key roles during the N 2 O production and consumption in the lake. A combination of isotopic and microbial analysis has been used to determine the denitrification and N 2 O reduction activities in water and sediments . However, no reasonable linear correlations were found between the δ 15 N and δ 18 O values of the NO 3 –N and N 2 O and their concentrations in spring, indicating restrained bacterial denitrification and N 2 O consumption with increasing algal accumulation (Table and Figure S3).…”

Section: Discussionmentioning

confidence: 99%

“…A combination of isotopic and microbial analysis has been used to determine the denitrification and N 2 O reduction activities in water and sediments. 57 However, no reasonable linear correlations were found between the δ 15 N and δ 18 O values of the NO 3 −N and N 2 O and their concentrations in spring, indicating restrained bacterial denitrification and N 2 O consumption with increasing algal accumulation (Table 2 and Figure S3). As a result, the algal bloom inhibited the denitrification and N 2 O reduction in the sediments, thus leading to low N 2 O concentrations and N 2 O fluxes.…”

Section: Microbial-driven N 2 O Production and Consumption Mechanismsmentioning

confidence: 98%

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Nitrification Regulates the Spatiotemporal Variability of N₂O Emissions in a Eutrophic Lake

Liang

Wang

Gao

et al. 2022

Environ. Sci. Technol.

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Nitrous oxide (N 2 O) emissions from lakes exhibit significant spatiotemporal heterogeneity, and quantitative identification of the different N 2 O production processes is greatly limited, causing the role of nitrification to be undervalued or ignored in models of a lake's N 2 O emissions. Here, the contributions of nitrification and denitrification to N 2 O production were quantitatively assessed in the eutrophic Lake Taihu using molecular biology and isotope mapping techniques. The N 2 O fluxes ranged from −41.48 to 28.84 μmol m −2 d −1 in the lake, with lower N 2 O concentrations being observed in spring and summer and significantly higher N 2 O emissions being observed in autumn and winter. The 15 N site preference and relevant isotopic evidence demonstrated that denitrification contributed approximately 90% of the lake's gross N 2 O production during summer and autumn, 27−83% of which was simultaneously eliminated via N 2 O reduction. Surprisingly, nitrification seemed to act as a key process promoting N 2 O production and contributing to the lake as a source of N 2 O emissions. A combination of N 2 O isotopocule-based approaches and molecular techniques can be used to determine the precise characteristics of microbial N 2 O production and consumption in eutrophic lakes. The results of this study provide a basis for accurately assessing N 2 O emissions from lakes at the regional and global scales.

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Section: Discussionmentioning

confidence: 99%

Section: Microbial-driven N 2 O Production and Consumption Mechanismsmentioning

confidence: 98%

Nitrification Regulates the Spatiotemporal Variability of N₂O Emissions in a Eutrophic Lake

Liang

Wang

Gao

et al. 2022

Environ. Sci. Technol.

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“…Higher N 2 O emissions after rewetting were also correlated with increased DNRA (dissimilatory nitrate reduction to ammonium; Espenberg et al 2018 ; Jahangir et al 2020 ; Masta et al 2022 ). nrfA (cytochrome c nitrite reductase) gene copies were positively correlated with N 2 O emissions both in pristine and rewetted peatlands, further highlighting the contribution of this process to N 2 O fluxes.…”

Section: Belowground Microbial Processes Involved In Ghg Dynamicsmentioning

confidence: 98%

“…To the best of our knowledge, the studies by Masta et al ( 2022 and 2023 ) represent the only ones to date linking N 2 O fluxes with ammonia oxidation (by using nitrification gene amoA abundances as indicator) in rewetted peatlands (Table S2 ). There, ammonia oxidation was estimated to be a source for N 2 O, secondary to denitrification.…”

Section: Belowground Microbial Processes Involved In Ghg Dynamicsmentioning

confidence: 99%

Unraveling microbial processes involved in carbon and nitrogen cycling and greenhouse gas emissions in rewetted peatlands by molecular biology

Gios,

Verbruggen,

Audet

et al. 2024

Biogeochemistry

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Restoration of drained peatlands through rewetting has recently emerged as a prevailing strategy to mitigate excessive greenhouse gas emissions and re-establish the vital carbon sequestration capacity of peatlands. Rewetting can help to restore vegetation communities and biodiversity, while still allowing for extensive agricultural management such as paludiculture. Belowground processes governing carbon fluxes and greenhouse gas dynamics are mediated by a complex network of microbial communities and processes. Our understanding of this complexity and its multi-factorial controls in rewetted peatlands is limited. Here, we summarize the research regarding the role of soil microbial communities and functions in driving carbon and nutrient cycling in rewetted peatlands including the use of molecular biology techniques in understanding biogeochemical processes linked to greenhouse gas fluxes. We emphasize that rapidly advancing molecular biology approaches, such as high-throughput sequencing, are powerful tools helping to elucidate the dynamics of key biogeochemical processes when combined with isotope tracing and greenhouse gas measuring techniques. Insights gained from the gathered studies can help inform efficient monitoring practices for rewetted peatlands and the development of climate-smart restoration and management strategies.

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“…Current models and policies lack specific data on the source and sink processes of N 2 O. 12 Previous studies that examine N 2 O emissions in field settings always only provide simplified assumptions about reaction pathways based on the relationships between concentrations of N 2 O and substrates (e.g., NH 4 + and NO 3 − ) or other environmental variables of overlying water that favor such pathways. 4 These analyses are far from able to point to the contributions of specific N 2 O production pathways and to differentiate the role of N 2 O reduction.…”

Section: Introductionmentioning

confidence: 99%

Tracing Microbial Production and Consumption Sources of N₂O in Rivers on the Qinghai-Tibet Plateau via Isotopocule and Functional Microbe Analyses

Chen

Zhang

Liu

et al. 2023

Environ. Sci. Technol.

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Nitrous oxide (N 2 O), a potent greenhouse gas, is produced in rivers through a series of microbial metabolic pathways. However, the microbial source of N 2 O production and the degree of N 2 O reduction in river systems are not well understood and quantified. This work investigated isotopic compositions (δ 15 N-N 2 O and δ 18 O-N 2 O) and N 2 O site preference as well as N 2 O-related microbial features, thereby differentiating the importance of nitrification, denitrification, and N 2 O reduction in controlling N 2 O emissions from five rivers on the eastern Qinghai-Tibet Plateau (EQTP). The average N 2 O concentration in overlying water (15.2 nmol L −1 ) was close to that in porewater (17.5 nmol L −1 ), suggesting that both overlying water and sediment are potentially important sources of N 2 O. Canonical and nitrifier denitrification dominated riverine N 2 O production, with contribution being approximately 90%. Nitrification is a nonnegligible source of N 2 O production, and N 2 O concentration was positively correlated with nitrification genetic potential. The degree of N 2 O reduction ranged from 78.1 to 94.1% (averaging 90%), significantly exceeding the reported values (averaging 70%) in other freshwaters, which was attributed to the higher ratios of organic carbon to nitrogen and lower ratio of (nirS + nirK)/nosZ in EQTP rivers. This study indicates that a combination of isotopic and isotopocule values with functional microbe analysis is useful for quantifying the microbial sources of N 2 O in rivers, and the intense microbial reduction of N 2 O significantly accounts for the low N 2 O emissions observed in EQTP rivers, suggesting that both the production and consumption of N 2 O in rivers should be considered in the future.

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Integrated isotope and microbiome analysis indicates dominance of denitrification in N2O production after rewetting of drained fen peat

Cited by 13 publications

References 63 publications

Nitrification Regulates the Spatiotemporal Variability of N₂O Emissions in a Eutrophic Lake

Nitrification Regulates the Spatiotemporal Variability of N₂O Emissions in a Eutrophic Lake

Unraveling microbial processes involved in carbon and nitrogen cycling and greenhouse gas emissions in rewetted peatlands by molecular biology

Tracing Microbial Production and Consumption Sources of N₂O in Rivers on the Qinghai-Tibet Plateau via Isotopocule and Functional Microbe Analyses

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Integrated isotope and microbiome analysis indicates dominance of denitrification in N2O production after rewetting of drained fen peat

Cited by 13 publications

References 63 publications

Nitrification Regulates the Spatiotemporal Variability of N2O Emissions in a Eutrophic Lake

Nitrification Regulates the Spatiotemporal Variability of N2O Emissions in a Eutrophic Lake

Unraveling microbial processes involved in carbon and nitrogen cycling and greenhouse gas emissions in rewetted peatlands by molecular biology

Tracing Microbial Production and Consumption Sources of N2O in Rivers on the Qinghai-Tibet Plateau via Isotopocule and Functional Microbe Analyses

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Product

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About

Nitrification Regulates the Spatiotemporal Variability of N₂O Emissions in a Eutrophic Lake

Nitrification Regulates the Spatiotemporal Variability of N₂O Emissions in a Eutrophic Lake

Tracing Microbial Production and Consumption Sources of N₂O in Rivers on the Qinghai-Tibet Plateau via Isotopocule and Functional Microbe Analyses