Integrated Immunoassay System Using Multichannel Microchip for Simultaneous Determination

Sato, Katsuaki; Yamanaka, Maho; Takahashi, Hiroyuki; Uchiyama, Kenji; Tokeshi, Manabu; Katou, Hajime; Kimura, Hiroko; Kitamori, Takehiko

doi:10.1007/978-94-010-1015-3_218

Cited by 2 publications

(2 citation statements)

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“…Thus, aggregated beads reduce the remaining free volume of the aggregation chamber and locally elevate the hydrodynamic resistance of the outlet geometry. In the region of a porous media the Navier-Stokes equation is extended to (1) whereas the additional parameters porosity f, effective viscosity h e , Forchheimer-coefficient a, and permeability [K] have to be taken into account.…”

Section: The Model Of the Porous Mediamentioning

confidence: 99%

“…Microfluidic technologies are increasingly used to miniaturize analytical instruments to form so-called "lab-on-a-chip" devices. 1,2,3,4 Striking advantages of these lab-on-a-chip concepts are the small volume of sample and reagents as well as the increased process integration and automation on behalf of the technological backend. For the consumer, this means an enhanced ease of use, faster time-to-results, and, eventually, appreciable cost savings.…”

Section: Introductionmentioning

confidence: 99%

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Aggregation of bead-monolayers in flat microfluidic chambers – simulation by the model of porous media

et al. 2004

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In this paper, we for the first time simulate the process of hydrodynamic bead aggregation in a flat micro-fluidic chamber by a porous-media model in an iterative routine. This allows us to optimize the chamber design of our recently developed experimental method to form periodical monolayers from the flow of bead suspension. Periodical monolayers are advantageous for parallel assay formats since they enhance the mechanical rigidity of the aggregated pattern. This is important to avoid a spatial rearrangement along various steps of a read-out procedure which would impair the correlation between measurements. Furthermore, the monolayer formation guarantees the individual optical accessibility of all probe beads. By modelling the monolayers with porous media, we can drastically reduce the degrees of freedom in a two-phase, multi-particle problem. This way, we are able to compute stationary hydrodynamic flow patterns in the chamber. In order to simulate the complete filling process from these stationary solutions, we developed an iterative master routine which takes the transient aggregation pattern as the initial condition, then evaluates the placement of the newly introduced beads, and finally converts the points of aggregation into porous media.

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Section: The Model Of the Porous Mediamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%