Integrated electrochemical measurement of endothelial permeability in a 3D hydrogel-based microfluidic vascular model

Wong, Jeremy; Mohan, Michael; Young, Elton T.; Simmons, Craig A.

doi:10.1016/j.bios.2019.111757

Cited by 48 publications

(38 citation statements)

References 34 publications

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“…Then we focus on microfluidic-based scaffolds. As discussed, these devices allow creating complex multifunctional devices that mimic tissue confinement and recapitulating physiological flows, gradients, permeability and/or crosstalk between different cells or barriers, e.g., the BM or vascular walls [ 191 ]. Microfluidic devices can also integrate mechanical or electrical actuators to monitor and trigger bioactive cues [ 191 , 192 ].…”

Section: Fabrication Of 3d Scaffolds: Microfluidics and Bioprintinmentioning

confidence: 99%

“…As discussed, these devices allow creating complex multifunctional devices that mimic tissue confinement and recapitulating physiological flows, gradients, permeability and/or crosstalk between different cells or barriers, e.g., the BM or vascular walls [ 191 ]. Microfluidic devices can also integrate mechanical or electrical actuators to monitor and trigger bioactive cues [ 191 , 192 ]. Both microfluidics and bioprinting techniques seem especially appropriate to fabricate hydrogel-based scaffolds with controlled properties to mimic the structure and properties of the ECM for migration assays.…”

Section: Fabrication Of 3d Scaffolds: Microfluidics and Bioprintinmentioning

confidence: 99%

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Modeling the Mechanobiology of Cancer Cell Migration Using 3D Biomimetic Hydrogels

Morales

Cortés-Domínguez

Ortiz‐de‐Solórzano

2021

Gels

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Understanding how cancer cells migrate, and how this migration is affected by the mechanical and chemical composition of the extracellular matrix (ECM) is critical to investigate and possibly interfere with the metastatic process, which is responsible for most cancer-related deaths. In this article we review the state of the art about the use of hydrogel-based three-dimensional (3D) scaffolds as artificial platforms to model the mechanobiology of cancer cell migration. We start by briefly reviewing the concept and composition of the extracellular matrix (ECM) and the materials commonly used to recreate the cancerous ECM. Then we summarize the most relevant knowledge about the mechanobiology of cancer cell migration that has been obtained using 3D hydrogel scaffolds, and relate those discoveries to what has been observed in the clinical management of solid tumors. Finally, we review some recent methodological developments, specifically the use of novel bioprinting techniques and microfluidics to create realistic hydrogel-based models of the cancer ECM, and some of their applications in the context of the study of cancer cell migration.

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Section: Fabrication Of 3d Scaffolds: Microfluidics and Bioprintinmentioning

confidence: 99%

Section: Fabrication Of 3d Scaffolds: Microfluidics and Bioprintinmentioning

confidence: 99%

Modeling the Mechanobiology of Cancer Cell Migration Using 3D Biomimetic Hydrogels

Morales

Cortés-Domínguez

Ortiz‐de‐Solórzano

2021

Gels

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show abstract

“…Their technique allowed the repaid recovery of the cells within 10 min with high viability for both cell types. A 3D hydrogel based vascular model was designed by Wong et al 75 (Fig. 6D) to study the electrochemical permeability of endothelial cells in a microuidic platform.…”

Section: Cell Culture In Hydrogelmicrofluidic Matricesmentioning

confidence: 99%

Hydrogels as artificial matrices for cell seeding in microfluidic devices

Akther

Little

et al. 2020

RSC Adv.

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“…One predictor of endothelial dysfunction is abnormal endothelial permeability (Gimbrone and García-Cardeña, 2016). Investigators developed an electrochemical assay for endothelial permeability in a microfluidic design (Wong et al, 2020), which bypasses the need for fluorescent tracers and imaging-based analysis. Another study employed tissue chips to study the abnormal endothelial permeability associated with sickle cell disease (Qiu et al, 2018).…”

Section: Vascular Tissue Chipsmentioning

confidence: 99%

Engineering Cardiovascular Tissue Chips for Disease Modeling and Drug Screening Applications

Chan

Huang

2021

Front. Bioeng. Biotechnol.

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In recent years, the cost of drug discovery and development have been progressively increasing, but the number of drugs approved for treatment of cardiovascular diseases (CVDs) has been limited. Current in vitro models for drug development do not sufficiently ensure safety and efficacy, owing to their lack of physiological relevance. On the other hand, preclinical animal models are extremely costly and present problems of inaccuracy due to species differences. To address these limitations, tissue chips offer the opportunity to emulate physiological and pathological tissue processes in a biomimetic in vitro platform. Tissue chips enable in vitro modeling of CVDs to give mechanistic insights, and they can also be a powerful approach for drug screening applications. Here, we review recent advances in CVD modeling using tissue chips and their applications in drug screening.

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Integrated electrochemical measurement of endothelial permeability in a 3D hydrogel-based microfluidic vascular model

Cited by 48 publications

References 34 publications

Modeling the Mechanobiology of Cancer Cell Migration Using 3D Biomimetic Hydrogels

Modeling the Mechanobiology of Cancer Cell Migration Using 3D Biomimetic Hydrogels

Hydrogels as artificial matrices for cell seeding in microfluidic devices

Engineering Cardiovascular Tissue Chips for Disease Modeling and Drug Screening Applications

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