Integrated eco-physiological, biochemical, and molecular biological analyses of selenium fortification mechanism in alfalfa

Hu, Jinke; Hu, Huafeng; Li, Yan; Xiang, Meiling; Wang, Dezhen

doi:10.1007/s00425-022-04027-6

Cited by 5 publications

(2 citation statements)

References 77 publications

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“…Nano-Se reduced the leaf MDA content by 21–36% at 0 dpi, and by 30 and 48% at 10 dpi in cultivars HMC and JX, respectively (C). Wang et al [ 60 ] reported that Na 2 SeO 3 reduced H 2 O 2 and MDA levels in alfalfa and Cunha et al [ 61 ] demonstrated that Na 2 SeO 4 enhanced enzymatic and non-enzymatic antioxidant metabolism in peanut plants.…”

Section: Resultsmentioning

confidence: 99%

Nano-selenium enhances melon resistance to Podosphaera xanthii by enhancing the antioxidant capacity and promoting alterations in the polyamine, phenylpropanoid and hormone signaling pathways

Kang,

Wu,

Jia

et al. 2023

J Nanobiotechnol

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Powdery mildew is one of the main problematic diseases in melon production, requiring the use of chemical pesticides with disease-resistant cultivars for control. However, the often rapid acquisition of fungicidal resistance by mildew pathogens makes this practice unsustainable. The identification of crop treatments that can enhance resistance to powdery mildew resistance is therefore important to reduce melon crop attrition. This study indicates that the application of Nano-Se can reduce the powdery mildew disease index by 21–45%. The Nano-Se treatment reduced reactive oxygen species (ROS) and malondialdehyde (MDA) accumulation, with increases in glutathione (GSH), proline and 1,1-Diphenyl-2-picrylhydrazyl radical (DPPH). Increases were also observed in the activities and transcriptional levels of the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and peroxidase (POD). Assays with four different cultivars of melon with differing levels of mildew resistance demonstrated that relative to the control, the Nano-Se treatment resulted in larger responses to mildew infection, including increases in the levels of putrescine (PUT; 43–112%) and spermine (SPM; 36–118%), indoleacetic acid (IAA; 43–172%) and salicylic acid (SA; 24–73%), the activities of phenylalanine ammonium lyase (PAL), trans-cinnamate 4-hydroxylase (C4H) and 4-coumarate: Co A ligase (4CL) of the phenylpropanoid pathway (22–38%, 24–126% and 19–64%, respectively). Key genes in the polyamine and phenylpropanoid pathway were also upregulated. These results indicate that the foliar application of Nano-Se improved melon defenses against powdery mildew infection, with a significant reduction in mildew disease development. Graphical Abstract

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Section: Resultsmentioning

confidence: 99%

Nano-selenium enhances melon resistance to Podosphaera xanthii by enhancing the antioxidant capacity and promoting alterations in the polyamine, phenylpropanoid and hormone signaling pathways

Kang,

Wu,

Jia

et al. 2023

J Nanobiotechnol

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“…Understanding the modulation of metabolic fluxes during environmental stress is essential to have a broad understanding of how plants adapt to these conditions [ 56 , 57 , 58 , 59 , 60 ]. In recent years, several studies on the effect of mineral nutrition on metabolic fluxes have been carried out in plants [ 25 , 61 , 62 , 63 , 64 ]. In an investigation of the response of potato cell cultures to Pi deprivation, we showed that the activity of a large number of C metabolism enzymes was increased by Pi deprivation [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

Carbon Fluxes in Potato (Solanum tuberosum) Remain Stable in Cell Cultures Exposed to Nutritional Phosphate Deficiency

He,

Dorion,

Carmona-Rojas

et al. 2023

Biology

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Nutritional phosphate deficiency is a major limitation to plant growth. Here, we monitored fluxes in pathways supporting respiratory metabolism in potato (Solanum tuberosum) cell cultures growing in control or limiting phosphate conditions. Sugar uptake was quantified using [U-14C]sucrose as precursor. Carbohydrate degradation through glycolysis and respiratory pathways was estimated using the catabolism of [U-14C]sucrose to 14CO2. Anaplerotic carbon flux was assessed by labeling with NaH14CO3. The data showed that these metabolic fluxes displayed distinct patterns over culture time. However, phosphate depletion had relatively little impact on the various fluxes. Sucrose uptake was higher during the first six days of culture, followed by a decline, which was steeper in Pi-sufficient cells. Anaplerotic pathway flux was more important at day three and decreased thereafter. In contrast, the flux between sucrose and CO2 was at a maximum in the mid-log phase of the culture, with a peak at Day 6. Metabolization of [U-14C]sucrose into neutral, basic and acidic fractions was also unaffected by phosphate nutrition. Hence, the well-documented changes in central metabolism enzymes activities in response to Pi deficiency do not drastically modify metabolic fluxes, but rather result in the maintenance of the carbon fluxes that support respiration.

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Selenium in plants: A nexus of growth, antioxidants, and phytohormones

Ikram,

Li,

Lin

et al. 2024

Journal of Plant Physiology

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Integrated eco-physiological, biochemical, and molecular biological analyses of selenium fortification mechanism in alfalfa

Cited by 5 publications

References 77 publications

Nano-selenium enhances melon resistance to Podosphaera xanthii by enhancing the antioxidant capacity and promoting alterations in the polyamine, phenylpropanoid and hormone signaling pathways

Nano-selenium enhances melon resistance to Podosphaera xanthii by enhancing the antioxidant capacity and promoting alterations in the polyamine, phenylpropanoid and hormone signaling pathways

Carbon Fluxes in Potato (Solanum tuberosum) Remain Stable in Cell Cultures Exposed to Nutritional Phosphate Deficiency

Selenium in plants: A nexus of growth, antioxidants, and phytohormones

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