Integrated Coulter counter based on 2-dimensional liquid aperture control

Nieuwenhuis, J.H.; Köhl, F.; Bastemeijer, J.; Sarro, P.M.; Vellekoop, Michael J.

doi:10.1016/j.snb.2003.10.017

Cited by 69 publications

(49 citation statements)

References 11 publications

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“…With two symmetric RPS channels on a microfluidic chip and a differential amplifier, this detection limit can be reduced to 1% [34,35]. To reduce clogging, the effective sampling aperture can be reduced without reducing the actual aperture by reducing the conductivity of the sheath flow [36,37]. With the differential amplifier, or the less conductive sheath fluid, a pore in the order of 5 lm enables the sizing of single vesicles with a diameter of 50 nm to 5 lm.…”

Section: Flow Cytometrymentioning

confidence: 99%

Innovation in detection of microparticles and exosomes

Pol

Coumans

Varga

et al. 2013

Journal of Thrombosis and Haemostasis

223

194

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Summary. Cell-derived or extracellular vesicles, including microparticles and exosomes, are abundantly present in body fluids such as blood. Although such vesicles have gained strong clinical and scientific interest, their detection is difficult because many vesicles are extremely small with a diameter of less than 100 nm, and, moreover, these vesicles have a low refractive index and are heterogeneous in both size and composition. In this review, we focus on the relatively high throughput detection of vesicles in suspension by flow cytometry, resistive pulse sensing, and nanoparticle tracking analysis, and we will discuss their applicability and limitations. Finally, we discuss four methods that are not commercially available: Raman microspectroscopy, micro nuclear magnetic resonance, small-angle X-ray scattering (SAXS), and anomalous SAXS. These methods are currently being explored to study vesicles and are likely to offer novel information for future developments.

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Section: Flow Cytometrymentioning

confidence: 99%

Innovation in detection of microparticles and exosomes

Pol

Coumans

Varga

et al. 2013

Journal of Thrombosis and Haemostasis

223

194

View full text Add to dashboard Cite

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“…The most popular technique based on electrical measurement is the electrical sensing zone method (Electrozone Counter or Coulter Principle), in which particles are suspended in an electrolyte and forced to flow through a small orifice. The change in electrical resistivity of the solution between two electrodes placed on either side of the orifice allows the particle size to be determined [18][19][20]. This method has been applied to many applications but its main disadvantage is that particles may get trapped in the orifice and cause plugging.…”

Section: Introductionmentioning

confidence: 99%

In-situ particle sizing at millimeter scale from electrochemical noise: simulation and experiments

Yakdi

Huet

Ngo

2015

Electrochimica Acta

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Over the last few years, particle sizing techniques in multiphase flows based on optical technologies emerged as standard tools but the main disadvantage of these techniques is their dependence on the visibility of the measurement volume and on the focal distance. Thus, it is important to promote alternative techniques for particle sizing, and, moreover, able to work in hostile environment. This paper presents a single-particle sizing technique at a millimeter scale based on the measurement of the variation of the electrolyte resistance (ER) due to the passage of an insulating sphere between two electrodes immerged in a conductive solution. A theoretical model was proposed to determine the influence of the electrode size, the interelectrode distance, the size and the position of the sphere, on the electrolyte resistance. Experimental variations of ER due to the passage of spheres and measured by using a home-made electronic device are also presented in this paper. The excellent agreement obtained between the theoretical and experimental results allows validation of both model and experimental measurements. In addition, the technique was shown to be able to perform accurate measurements of the velocity of a ball falling in a liquid.

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“…However, there is a major difficulty associated with micro impedance sensing. The series metal-electrolyte impedance is inversely proportional to the electrode surface area so the downsizing unavoidably reduces the sensor sensitivity [4][5][6][7]. For examples, recent works on micro DC Coulter sensors were forced to stay with very large thin film electrode [8], macroscopic gold pin electrodes [9] and salt bridge with nonpolarizing Ag/AgCl electrodes [10].…”

Section: Introductionmentioning

confidence: 99%

“…Unfortunately there is an upper limit of the operation frequency imposed by the stray capacitance between sensing electrodes, which is resulted from coupling and non-ideal isolation. So to study particles with AC impedance sensing, the device is limited to a frequency range, which is high enough to bypass electrode double layer impedance and low enough that the stray capacitance does not play a significant role in the overall system impedance [7]. Micro AC impedance sensors have been attempted to sense cell suspensions and single cells.…”

Section: Introductionmentioning

confidence: 99%

Human Blood Cell Sensing with Platinum Black Electroplated Impedance Sensor

Zheng

Nandra

Tai

2007

2007 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems

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Abstract-AC impedance sensing is an important method for biological cell analysis in flow cytometry. For micro impedance cell sensors, downsizing electrodes increases the double layer impedance of the metal-electrolyte interface, thus leaves no sensing zone in frequency domain and reduces the sensitivity significantly. We proposed using platinum black electroplated electrodes to solve the problem. In this paper, using this technique we demonstrated human blood cell sensing with high signal to noise ratio.

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Integrated Coulter counter based on 2-dimensional liquid aperture control

Cited by 69 publications

References 11 publications

Innovation in detection of microparticles and exosomes

Innovation in detection of microparticles and exosomes

In-situ particle sizing at millimeter scale from electrochemical noise: simulation and experiments

Human Blood Cell Sensing with Platinum Black Electroplated Impedance Sensor

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