Integrated control of blue mould using new fungicides and biocontrol yeasts lowers levels of fungicide residues and patulin contamination in apples

Lima, Giuseppe; Castoria, Raffaello; Curtis, Filippo De; Raiola, Assunta; Ritieni, Alberto; Cicco, V. De

doi:10.1016/j.postharvbio.2010.12.010

Cited by 78 publications

(63 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The potential of including BCAs with mefenoxam can be in form of rotations with mefenoxam to reduce the frequency of fungicide applications for a more environmentally friendly, long lasting, consistent, and effective control of P. capsici. Similar results on BCA have been reported in other studies [50][51][52][53][54].…”

Section: Discussionsupporting

confidence: 91%

Evaluation of Selected Bacterial Endophytes for Biocontrol Potential against Phytophthora Blight of Bell Pepper (Capsicum annuum L.)

A¹,

Mt²

2017

J Plant Pathol Microbiol

View full text Add to dashboard Cite

Phytophthora blight, caused by Phytophthora capsici, is the most destructive disease of bell pepper in the United States. The effectiveness of current management strategies is limited by the long-term survival of oospores, wide host range, aggressive fungicide-resistant isolates, and lack of resistant cultivars with acceptable agronomic traits. Biological control is a viable alternative, and the use of endophytic microorganisms as biological control agents (BCA) has attracted widespread attention because they colonize the same ecological niches as plant pathogens. Seven isolates of bacterial endophytes in three genera Serratia (B17B), Enterobacter (E), and Bacillus (IMC8, Y, Ps, Psl and Prt), isolated from papaya, snap bean and flowering dogwood were evaluated for effects on P. capsici mycelial growth in vitro, and on disease severity and plant growth in greenhouse environment. All isolates significantly inhibited the mycelial growth of P. capsici with Ps, Psl and Prt having superior inhibitory effects. Seed treatments with Ps, Psl and Prt followed by plant inoculation with P. capsici reduced disease severity, and significantly increased plant shoot height, fresh weight, and fruit yield (number and weight) with Ps and Prt being slightly superior to Psl. Compatibility tests between isolates showed that only B17B and Y were compatible with each other. Sensitivity tests to different levels of mefenoxam (Ridomil Gold SL) showed that Ps, Psl and Prt were tolerant to the fungicide, while P. capsici was highly sensitive. Thus, Ps, Psl and Prt can be used in rotation with mefenoxam to reduce the frequency of fungicide usage for a more environmentally friendly, long lasting, consistent, and effective control of phytophthora blight. Overall, all isolates tested are potential BCAs against P. capsici, but more greenhouse and field studies are required to confirm in vitro results for all isolates that showed good potential in in vitro studies.

show abstract

Section: Discussionsupporting

confidence: 91%

Evaluation of Selected Bacterial Endophytes for Biocontrol Potential against Phytophthora Blight of Bell Pepper (Capsicum annuum L.)

A¹,

Mt²

2017

J Plant Pathol Microbiol

View full text Add to dashboard Cite

show abstract

“…Among different microbial antagonists, yeasts have been of special interest due to their general lack of antibiotic production (Nally et al 2015;Pantelides et al 2015;Parafati et al 2015), rapid colonization of the fruit surface and wound sites (Castoria et al 2005), abiotic stress tolerance Wang et al 2010a), and compatibility with fungicides and other chemicals (Lima et al 2011;Lu et al 2014). Therefore, antagonistic yeasts are considered to have significant potential for reducing or replacing the use of chemical fungicides (Cao et al 2013;El Ghaouth et al 2003;Janisiewicz et al 2014;Lu et al 2014;Zhu et al 2015).…”

Section: Introductionmentioning

confidence: 99%

The ability of a cold-adapted Rhodotorula mucilaginosa strain from Tibet to control blue mold in pear fruit

Yan

Wilson³

et al. 2015

Antonie van Leeuwenhoek

View full text Add to dashboard Cite

Cold-adapted yeasts were isolated from soil samples collected in Tibet and evaluated as potential biocontrol agents against blue mold (Penicillium expansum) of pear fruit in cold storage. YC1, an isolate identified as Rhodotorula mucilaginosa, was found to exhibit the greatest biocontrol activity among the different isolates that were screened. A washed cell suspension of YC1 exhibited the best biocontrol activity among three different preparations that were used in the current study. A concentration of 10(8) cells/ml reduced the incidence of decay to 35 %, compared to the control where decay incidence was 100 %. A higher intracellular level of trehalose and a higher proportion of polyunsaturated acids present in YC1, was associated with increased the tolerance of this strain to low temperatures, relative to the other strains that were evaluated. The increased tolerance to low temperature allowed the YC1 strain of yeast to more effectively compete for nutrients and space in wounded pear fruit that had been inoculated with spores of P. expansum and placed in cold storage. The present study demonstrated the ability to select cold-adapted yeasts from cold climates and use them as biocontrol agents of postharvest diseases of fruit placed in cold storage.

show abstract

“…For example, two biocontrol agents (BCAs), the basidiomycete yeasts Rhodosporidium kratochvilovae LS11 (originally named Rhodotorula glutinis) and Cryptococcus laurentii LS28, were effective in reducing the incidence of blue mold on apples with different mechanisms of action (6)(7)(8). The combination of these BCAs with food-grade additives or low doses of recently developed fungicides resulted in a significant rot reduction and in lower patulin contamination in apples (9,10).…”

mentioning

confidence: 99%

Searching for Genes Responsible for Patulin Degradation in a Biocontrol Yeast Provides Insight into the Basis for Resistance to This Mycotoxin

Ianiri

Idnurm

Wright

et al. 2013

Appl Environ Microbiol

View full text Add to dashboard Cite

f Patulin is a mycotoxin that contaminates pome fruits and derived products worldwide. Basidiomycete yeasts belonging to the subphylum Pucciniomycotina have been identified to have the ability to degrade this molecule efficiently and have been explored through different approaches to understand this degradation process. In this study, Sporobolomyces sp. strain IAM 13481 was found to be able to degrade patulin to form two different breakdown products, desoxypatulinic acid and (Z)-ascladiol. To gain insight into the genetic basis of tolerance and degradation of patulin, more than 3,000 transfer DNA (T-DNA) insertional mutants were generated in strain IAM 13481 and screened for the inability to degrade patulin using a bioassay based on the sensitivity of Escherichia coli to patulin. Thirteen mutants showing reduced growth in the presence of patulin were isolated and further characterized. Genes disrupted in patulin-sensitive mutants included homologs of Saccharomyces cerevisiae YCK2, PAC2, DAL5, and VPS8. The patulin-sensitive mutants also exhibited hypersensitivity to reactive oxygen species as well as genotoxic and cell wall-destabilizing agents, suggesting that the inactivated genes are essential for tolerating and overcoming the initial toxicity of patulin. These results support a model whereby patulin degradation occurs through a multistep process that includes an initial tolerance to patulin that utilizes processes common to other external stresses, followed by two separate pathways for degradation.

show abstract

Integrated control of blue mould using new fungicides and biocontrol yeasts lowers levels of fungicide residues and patulin contamination in apples

Cited by 78 publications

References 30 publications

Evaluation of Selected Bacterial Endophytes for Biocontrol Potential against Phytophthora Blight of Bell Pepper (Capsicum annuum L.)

Evaluation of Selected Bacterial Endophytes for Biocontrol Potential against Phytophthora Blight of Bell Pepper (Capsicum annuum L.)

The ability of a cold-adapted Rhodotorula mucilaginosa strain from Tibet to control blue mold in pear fruit

Searching for Genes Responsible for Patulin Degradation in a Biocontrol Yeast Provides Insight into the Basis for Resistance to This Mycotoxin

Contact Info

Product

Resources

About