Integrated bioprocess for structured lipids, emulsifiers and biodiesel production using crude acidic olive pomace oils

Heinzl, Giuditta C.; Mota, Danyelle Andrade; Martinis, Valentina; Martins, Ana Sofia; Soares, Cleide Mara Faria; Osório, Natália M.; Gominho, Jorge; Nampoothiri, K. Madhavan; Sukumaran, Rajeev; Pereira, Helena; Ferreira‐Dias, Suzana

doi:10.1016/j.biortech.2021.126646

Cited by 7 publications

(8 citation statements)

References 43 publications

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“…12 to 29% ( Table 1 ). Similar behavior was previously observed for Lipozyme RM IM in batch acidolysis of crude pomace oils with acidity ranging from 3.4 to 20% and caprylic or capric acids [ 38 ]. It is worth mentioning that the acidity of OPO is due to long-chain FFA in the reaction media, mainly oleic acid ( Table 1 ).…”

Section: Resultssupporting

confidence: 84%

“…In batch bioreactors, Heinzl et al [ 38 ] observed that Lipozyme RM IM achieved similar new TAG yields (47.8–53.4%), either in the acidolysis of crude olive pomace oil with C8:0 or C10:0 or in the interesterification with the respective ethyl esters. Conversely, Lipozyme TL IM showed a better performance in batch interesterification than in acidolysis, and the reaction rates were always lower than with Lipozyme RM IM.…”

Section: Resultsmentioning

confidence: 99%

“…Conversely, Lipozyme TL IM showed a better performance in batch interesterification than in acidolysis, and the reaction rates were always lower than with Lipozyme RM IM. Moreover, Lipozyme TL IM showed higher affinity for C10EE over C8EE [ 38 ].…”

Section: Resultsmentioning

confidence: 99%

“…Other oils have been used as feedstock in SL production, with reactions catalyzed by Lipozyme RM IM or Lipozyme TL IM, such as grape seed oil [ 3 ] (34.53% C10:0 incorporation) or roasted crude sesame oil, rich in oleic acid [ 46 ] (C8:0 incorporation of 42.5%). In several studies, Lipozyme RM IM exhibited a higher degree of incorporation than Lipozyme TL IM [ 38 , 47 , 48 , 49 ].…”

Section: Resultsmentioning

confidence: 99%

“…Previous studies were performed by our group to valorize crude OPO as feedstock for the production of SLs, by batch interesterification of crude acidic olive pomace oils with caprylic and capric acids, or their ethyl esters, in solvent-free media. In these studies, Rhizopus oryzae lipase immobilized in magnetic nanoparticles or commercial immobilized lipases (Lipozyme RM IM and Lipozyme TL IM) were used as biocatalysts [ 4 , 38 ]. However, according to our knowledge, the production of these SLs with crude olive pomace oil, in continuous bioreactors, has not been studied yet.…”

Section: Introductionmentioning

confidence: 99%

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Continuous Production of Dietetic Structured Lipids Using Crude Acidic Olive Pomace Oils

et al. 2023

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Crude olive pomace oil (OPO) is a by-product of olive oil extraction. In this study, low-calorie structured triacylglycerols (TAGs) were produced by acidolysis of crude OPO with medium-chain fatty acids (caprylic, C8:0; capric, C10:0) or interesterification with their ethyl ester forms (C8EE, C10EE). These new TAGs present long-chain fatty acids (L) at position sn-2 and medium-chain fatty acids (M) at positions sn-1,3 (MLM). Crude OPO exhibited a high acidity (12.05–28.75% free fatty acids), and high contents of chlorophylls and oxidation products. Reactions were carried out continuously in a packed-bed bioreactor for 70 h, using sn-1,3 regioselective commercial immobilized lipases (Thermomyces lanuginosus lipase, Lipozyme TL IM; and Rhizomucor miehei lipase, Lipozyme RM IM), in solvent-free media at 40 °C. Lipozyme RM IM presented a higher affinity for C10:0 and C10EE. Lipozyme TL IM preferred C10:0 over C8:0 but C8EE over C10EE. Both biocatalysts showed a high activity and operational stability and were not affected by OPO acidity. The New TAG yields ranged 30–60 and the specific productivity ranged 0.96–1.87 g NewTAG/h.g biocatalyst. Lipozyme RM IM cost is more than seven-fold the Lipozyme TL IM cost. Therefore, using Lipozyme TL IM and crude acidic OPO in a continuous bioreactor will contribute to process sustainability for structured lipid production by lowering the cost of the biocatalyst and avoiding oil refining.

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Section: Resultssupporting

confidence: 84%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Continuous Production of Dietetic Structured Lipids Using Crude Acidic Olive Pomace Oils

et al. 2023

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Efficacy mechanisms research progress of the active components in the characteristic woody edible oils

Xu,

Wang,

Bai

et al. 2023

Food Frontiers

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Woody edible oils are a type of vegetable oil. Woody edible oils like olive oil have greater quantities of unsaturated fatty acids (UFAs), particularly essential FAs, as well as vitamin E, phytosterols, and other nutrients that are becoming more vital in human health. As a result, finding high‐quality woody oil resource plants is critical to ensuring enough edible oil supply. As six novel woody crops, Paeonia suffruticosa, Plukenetia volubilis, Acer truncatum, Olea europaea, Camellia sinensis, and Camellia oleifera are characterized by high oil production, widespread cultivation, adaptability, and various active ingredients. The six woody crop oils contain UFAs (e.g., α‐linolenic acid, oleic acid, and linoleic acid), vitamin E, polyphenols, phytosterols, and so forth. The presence of these active ingredients confers anti‐inflammatory, antioxidant, cholesterol and lipid metabolism regulating, blood lipid lowering, immune boosting, memory improving, intestinal flora regulating, and other properties to the oils, which are beneficial to body health. This article examined in depth the seed resources, FA composition, active component kinds, active ingredient efficacy mechanism, and physiological impacts of these six novel woody crop oils. These developments lay a solid platform for further study and development of these woody oil crops.

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Machine learning aided experimental approach for evaluating the growth kinetics of Candida antarctica for lipase production

Sarmah

Mehtab

Bugata

et al. 2022

Bioresource Technology

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Integrated bioprocess for structured lipids, emulsifiers and biodiesel production using crude acidic olive pomace oils

Cited by 7 publications

References 43 publications

Continuous Production of Dietetic Structured Lipids Using Crude Acidic Olive Pomace Oils

Continuous Production of Dietetic Structured Lipids Using Crude Acidic Olive Pomace Oils

Efficacy mechanisms research progress of the active components in the characteristic woody edible oils

Machine learning aided experimental approach for evaluating the growth kinetics of Candida antarctica for lipase production

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