Integrated biomarkers induced by chlorpyrifos in two different life stages of zebrafish ( Danio rerio ) for environmental risk assessment

Jeon, Hwang‐Ju; Lee, Yong-Ho; Kim, Myoung-Jin; Choi, Sung-Deuk; Park, Byung‐Jun; Lee, Sungeun

doi:10.1016/j.etap.2016.03.010

Cited by 41 publications

(23 citation statements)

References 43 publications

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“…We tested the LC50 of CPF to the adult zebrafish in a large rang of concentration and time. It was 0.6 mg/L after 72h exposure in static water adding CPF once at the beginning, which was the similar concentration as that reported by Jeon & Lee (LC50 was 0.6mg/l after 71.2h) [20]. For the exposure mortality experiment results, we noted there was a concentration dependent of EGCG combined with CPF.…”

Section: Discussionsupporting

confidence: 89%

“…In the independent tests, no zebrafish death was observed at the concentration of 0.10 ~ 1.0mg / L of EGCG during the exposing time of 120h and 2.0~10.0mg / L in 72 h. The LC50 of CPF was 0.6 mg / L after 72h, which was the similar concentration as that reported by Jeon & Lee (LC50 was 0.6mg/l after 71.2h) [20]. After 24 h exposure, the LD50 was 1.0 mg / L in current tests (Figure 1).…”

Section: Independent and Combinational Exposing And Lethal Testssupporting

confidence: 84%

“…Given the LC 50 at 0.5 ~0.7 mg / L after the exposure time of 72h were reported in most researches and the LC 50 at 0.6 mg / L detected in current test was corresponding with the results reported recently [20], we trialed the effects of 0.5, 0.6, 0.7 mg / L of CPF on AChE activity. As can be seen in Figure 3, EGCG greatly changed the concentration of AChE in the brain of adult zebrafish.…”

Section: Analysis Of Acetylcholinesterase Ache Activitysupporting

confidence: 83%

“…Tests were set according to the literature [20], and the adding concentrations of CPF and EGCG were 0.010~ 1.0mg/L and 0.010~ 10.0mg/L, respectively (seen in table 1 for the specific concentration), and the exposing time was 120h in the independent tests. For the combined effects of EGCG and CPF, the factorial analysis of CPF (3)*EGCG (7) was designed.…”

Section: Toxicity Test Methods and Sample Collectionmentioning

confidence: 99%

“…Many features of zebrafish (Danio rerio) have made it become a model system for the examination of environmental toxicology [14]. It also help to determine the critical neuro-developmental processes impacted by organophosphorus pesticides, Chlorpyrifos as an instance [10,[19][20][21].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Effects of EGCG and Chlorpyrifos on the Mortality, AChE and GSH of Adult Zebrafish: Independent and Combination

Zhang

Gao

et al. 2018

IOP Conf. Ser.: Mater. Sci. Eng.

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Section: Discussionsupporting

confidence: 89%

Section: Independent and Combinational Exposing And Lethal Testssupporting

confidence: 84%

Section: Analysis Of Acetylcholinesterase Ache Activitysupporting

confidence: 83%

Section: Toxicity Test Methods and Sample Collectionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Effects of EGCG and Chlorpyrifos on the Mortality, AChE and GSH of Adult Zebrafish: Independent and Combination

Zhang

Gao

et al. 2018

IOP Conf. Ser.: Mater. Sci. Eng.

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Relative gene expression analysis of catalase in environmental RNA from Japanese medaka exposed to toxic chemicals

Hiki,

Watanabe,

Yamamoto

2024

Environmental DNA

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Environmental RNA (eRNA) has the potential as a non‐invasive tool for assessing the physiological status of macro‐organisms, yet the quantitative method for relative gene expression analysis is still underexplored. To bridge this gap, this study introduces a relative quantification method for eRNA, employing quantitative PCR (qPCR) to evaluate the expression of the antioxidant gene, catalase (cat), in the Japanese medaka Oryzias latipes exposed to two toxic chemicals for 96 h: chlorpyrifos (CPS) and carbamazepine. Our results showed that, in eRNA, genes frequently used as a reference for tissue or cells in conventional expression analysis correlated with each other. Also, one of them (elfa) exhibited less variability, showing its potential suitability as a reference gene in eRNA analyses. Additionally, cat expression levels in eRNA increased with increasing CPS concentrations, in a concentration‐response manner. These results suggest the promising potential of qPCR applications for eRNA in the monitoring of an organism's health and response to environmental changes. However, we observed disparities in gene expression levels of cat and beta‐actin (actb) between eRNA and whole fish body, indicating eRNA might have a biased origin. Further research is needed to uncover the origin of eRNA and determine the limitations and applicability domain of eRNA analysis.

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Using Transcriptomics and Metabolomics to Understand Species Differences in Sensitivity to Chlorpyrifos in Japanese Quail and Double‐Crested Cormorant Embryos

Desforges

Legrand

Boulager

et al. 2021

Enviro Toxic and Chemistry

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Modern 21st‐century toxicity testing makes use of omics technologies to address critical questions in toxicology and chemical management. Of interest are questions relating to chemical mechanisms of toxicity, differences in species sensitivity, and translation of molecular effects to observable apical endpoints. Our study addressed these questions by comparing apical outcomes and multiple omics responses in early–life stage exposure studies with Japanese quail (Coturnix japonica) and double‐crested cormorant (Phalacrocorax auritus), representing a model and ecological species, respectively. Specifically, we investigated the dose‐dependent response of apical outcomes as well as transcriptomics and metabolomics in the liver of each species exposed to chlorpyrifos, a widely used organophosphate pesticide. Our results revealed a clear pattern of dose‐dependent disruption of gene expression and metabolic profiles in Japanese quail but not double‐crested cormorant at similar chlorpyrifos exposure concentrations. The difference in sensitivity between species was likely due to higher metabolic transformation of chlorpyrifos in Japanese quail compared to double‐crested cormorant. The most impacted biological pathways after chlorpyrifos exposure in Japanese quail included hepatic metabolism, oxidative stress, endocrine disruption (steroid and nonsteroid hormones), and metabolic disease (lipid and fatty acid metabolism). Importantly, we show consistent responses across biological scales, suggesting that significant disruption at the level of gene expression and metabolite profiles leads to observable apical responses at the organism level. Our study demonstrates the utility of evaluating effects at multiple biological levels of organization to understand how modern toxicity testing relates to outcomes of regulatory relevance, while also highlighting important, yet poorly understood, species differences in sensitivity to chemical exposure. Environ Toxicol Chem 2021;40:3019–3033. © 2021 SETAC

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Integrated biomarkers induced by chlorpyrifos in two different life stages of zebrafish ( Danio rerio ) for environmental risk assessment

Cited by 41 publications

References 43 publications

Effects of EGCG and Chlorpyrifos on the Mortality, AChE and GSH of Adult Zebrafish: Independent and Combination

Effects of EGCG and Chlorpyrifos on the Mortality, AChE and GSH of Adult Zebrafish: Independent and Combination

Relative gene expression analysis of catalase in environmental RNA from Japanese medaka exposed to toxic chemicals

Using Transcriptomics and Metabolomics to Understand Species Differences in Sensitivity to Chlorpyrifos in Japanese Quail and Double‐Crested Cormorant Embryos

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