Integrated biomarker responses of an estuarine invertebrate to high abiotic stress and decreased metal contamination

Rodrigues, Aurélie Pinto; Oliva-Teles, Maria Teresa; Mesquita, Sofia Raquel; Delerue–Matos, Cristina; Guimarães, Laura

doi:10.1016/j.marenvres.2014.10.001

Cited by 20 publications

(8 citation statements)

References 55 publications

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“…8 In addition, some GST isozymes display peroxidase activity with respect to lipid hydroperoxides. 82,83 The ability of the liver to induce CAT and GST activities, as well as to increase the GSHt content, in response to Hg exposure/accumulation or abiotic variations evidenced an elevated antioxidant protection, which is characteristic of this organ. Previous studies have also shown that exposure to Hg induces GST activity.…”

Section: Discussionmentioning

confidence: 99%

“…2,56,81 The reported alterations in oxidative stress biomarkers may also be associated with abiotic factors not related to contaminants, such as lower salinity and dissolved oxygen content as verified at LAR; previous studies have verified that these two variables influence both the toxicity of metals and oxidative stress responses. 82,83 The ability of the liver to induce CAT and GST activities, as well as to increase the GSHt content, in response to Hg exposure/accumulation or abiotic variations evidenced an elevated antioxidant protection, which is characteristic of this organ.…”

Section: Discussionmentioning

confidence: 99%

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Unravelling the mechanisms of mercury hepatotoxicity in wild fish (Liza aurata) through a triad approach: bioaccumulation, metabolomic profiles and oxidative stress

et al. 2015

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Clarification of the mechanisms of mercury (Hg) toxicity in fish remains challenging. An innovative approach to this clarification is the combined assessment of metabolomic shifts, alterations in the antioxidant system and the risk of oxidative damage, and Hg bioaccumulation. This strategy was applied to the livers of golden grey mullet (Liza aurata) inhabiting an Hg-contaminated system in Aveiro Lagoon, Portugal. Marked changes in both the metabolic profile and the response to antioxidants were observed in fish exposed to Hg in the environment, indicating their compromised state of health. The metabolomics analysis showed a clear differentiation between the contaminated and uncontaminated areas, revealing that Hg has multiple levels of impact, including membrane stabilization/degradation/repair processes, osmoregulation, energy metabolism, gene expression and antioxidant protection. Research on oxidative stress biomarkers showed that Hg triggered adaptive responses in the antioxidant system as seen by an increase in the activities of glutathione-S-transferase and catalase, as well as the total glutathione content, which compensated for a decrease in glutathione peroxidase activity. The integration of metabolomics and oxidative stress endpoints allowed the identification of reduced glutathione as a first line of defence against Hg and evidenced oxidative insults in cell membranes, recognized through the increase in phosphatidylcholine degradation products (phosphocholine and glycerophosphocholine). Despite these effects, the induction of lipid peroxidation was efficiently prevented. The triad approach used here was demonstrated to be sensitive and effective towards a mechanistically based assessment of Hg hepatotoxicity in fish.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Unravelling the mechanisms of mercury hepatotoxicity in wild fish (Liza aurata) through a triad approach: bioaccumulation, metabolomic profiles and oxidative stress

et al. 2015

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“…IBR appears as a useful tool to mark pollution in field assessments, as it reflects contaminant levels in sites, despite the variability of biomarkers used for its calculation. Field and biomonitoring studies, concerning environmental pollution, rely on IBR for result support [48][49][50][51][52]. Fewer laboratory studies testify IBR's applicability within the scope of treatment comparison [47,53].…”

Section: Propranololmentioning

confidence: 99%

Evaluation of Multivariate Biomarker Indexes Application in Ecotoxicity Tests with Marine Diatoms Exposed to Emerging Contaminants

Pires¹,

Novais²,

Lemos³

et al. 2021

Preprint

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Worldwide anthropogenic activities result in the production and release of poten-tially damaging toxic pollutants into ecosystems, thereby jeopardizing their health and continuity. Research studies and biomonitoring programs attend to this emerging problematic by applying and developing statistically relevant indexes that integrate complex biomarker response data to provide an holistic approach reflecting toxically induced alterations at the organism’s or population level. Ultimately, indexes allow simple result communication, enhancing policy makers understanding, so contributing for better resource and environmental managing policies. In this study three indexes , the Integrated Biomarker Response index (IBR), the Bioeffects Assessment Index (BAI) and the Principal Components Analysis (PCA), were evaluated for their sensitivity in revealing toxically induced stress patterns in cells of the diatom Phaeodactylum tricornutum under contaminant exposure. The set of biomarkers selected for index construction comprise the anti-oxidant enzymes APX, CAT and SOD, and the lipid peroxidation marker TBARS. Several significant inverse correlations with the concentration gradients applied, , was noticed for all indexes, though, the IBR excels for its reliability in delivering statistically significant dose-response patterns for four out of the five compounds tested.

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“…The multiple biomarker approach has been extensively used as an auxiliary approach coupled with traditional water quality monitoring to evaluate the biological impacts of environmental metal pollution on marine organisms . Biomarkers are the measurements at the molecular, biochemical, cellular or physiological level to indicate the magnitude of an organism's response to pollutants and are used as early warning signals of biological effects caused by environmental pollutants.…”

Section: Introductionmentioning

confidence: 99%

Physiological and cellular responses of oysters (Crassostrea hongkongensis) in a multimetal‐contaminated estuary

Wang

2016

Enviro Toxic and Chemistry

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The Pearl River estuary, southern China, suffers from multiple sources of metal contamination as a result of the rapid industrial development in the region; but the biological impacts of contamination remain unknown. In the present study, a euryhaline oyster, Crassostrea hongkongensis, was collected from different sites of the Pearl River estuary; and various physiological (heart rate, alkaline phosphatase as homeostatic regulation, and glycogen as energy reserve) and cytological (lysosomal membrane stability) biomarkers were quantified to assess this species as a potential bioindicator of metal pollution in contaminated areas. Large variations of metal accumulation levels in the oysters were documented, especially for copper (Cu), zinc (Zn), cadmium (Cd), chromium, and nickel (Ni). Among these metals, the authors demonstrated significant correlations between the digestive gland metal accumulation of Cu, Zn, and Ni and the cellular homeostasis (alkaline phosphatase) and glycogen reserves. Heart rate was positively correlated with Cd but negatively correlated with Cu and Zn concentrations in the gills. Lysosomal membrane stability was significantly inhibited at the most contaminated sites but had no relationship with the accumulated metal concentrations. These measurements indicate that multimetal contamination in the Pearl River estuary impacts the physiological and cytological performance of oysters. Environ Toxicol Chem 2016;35:2577-2586. © 2016 SETAC.

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Integrated biomarker responses of an estuarine invertebrate to high abiotic stress and decreased metal contamination

Cited by 20 publications

References 55 publications

Unravelling the mechanisms of mercury hepatotoxicity in wild fish (Liza aurata) through a triad approach: bioaccumulation, metabolomic profiles and oxidative stress

Unravelling the mechanisms of mercury hepatotoxicity in wild fish (Liza aurata) through a triad approach: bioaccumulation, metabolomic profiles and oxidative stress

Evaluation of Multivariate Biomarker Indexes Application in Ecotoxicity Tests with Marine Diatoms Exposed to Emerging Contaminants

Physiological and cellular responses of oysters (Crassostrea hongkongensis) in a multimetal‐contaminated estuary

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