Integrated biomarker response in catfish Hypostomus ancistroides by multivariate analysis in the Pirapó River, southern Brazil

Piscine micronucleus assay and the evidence of environmental degradation: the case of catfish from Brazilian tropical estuaries / Bioensaio de micronúcleo em peixes e evidências de degradação ambientai: o caso dos bagres de estuários tropicais brasileiros

“…Despite the low rates of MN and NA in this study, severe histological alterations, especially in the kidneys of ECR catfish, indicate chronic damages in these animals (Santana 2014), suggesting that contaminants are bioavailable but are not causing genotoxic effects to the catfish blood cells. This is in agreement with Ghisi et al (2016) whose results showed the necessity of a multivariate analysis, evaluating several biological parameters to obtain an integrated response to the effects of the environmental pollutants on the organisms, in order to give more precise diagnosis, more valid results and higher social credibility. Furthermore, high frequencies of MN (74.4 ‰) and NA (150.00 ‰) induction (Table II) were found to be the cause of reduction of 96% of the population of fish Labeo rohita in the River Chenab, Pakistan (Hussain et al 2018).…”

“…As occurred in the present study (Table I and Fig. 3), a higher frequency of NA than MN was found in polluted monitoring studies (Kirschbaum et al 2009, Osman et al, 2011, Azevedo et al 2012, Fatima et al 2015, Ghisi et al 2016, Gusso-Choueri et al 2016, Hussain et al 2018) and elevated standard deviation in both absolute frequencies was observed in some research (Andrade et al 2004, Gusso-Choueri et al 2016. The levels of MN in the ECR and the EPR (this study) were similar to the observed in catfish collected in Cananéia-Iguape-Peruíbe/SP (Gusso-Choueri et al 2016) and in Santos-São Vicente estuarine system/SP (Azevedo et al 2012), but the Ʃ MN+NA in fish from both estuaries were higher than those reported in our study (Table II) evidencing the impact of human activities, such as industrial and domestic sewage disposal, in Brazilian estuaries.…”

Use of liver histological alterations and erythrocytic nuclear abnormalities of two native fish in Kelani River, Sri Lanka as biomarkers for pollution impact assessments

“…Micronuclei can be formed as a result of chromosomal breaks or mitotic spindle dysfunctions (Al-Sabti & Metcalfe, 1995). Although the exact mechanisms of induction of erythrocytic nuclear abnormalities are less understood, they have been used as indicators of exposure to various genotoxic and mutagenic contaminants (Ghisi et al, 2016;Braham et al, 2017;Vieira et al, 2017). In the present study, nuclear abnormalities detected in the erythrocytes of the fish (Figure 3) were nuclear buds (Figures 3a2 and 3b1), notched nuclei ( Figures 3a1, 3a2 and 3b3), vacuolated nuclei ( Figure 3b1) and micronuclei (Figure 3b2).…”

“…Biomarkers are defined as changes in biological (molecular, cellular, physiological or behavioural) responses, which can be related to exposure to or toxic effects of chemicals in the environment (Peakall, 1994). Multi-biomarker approach in fish is recognised as a complementary tool for environmental monitoring studies to better appraise how pollutants affect aquatic ecosystems (Pathiratne et al, 2008;Ghisi et al, 2016;Vieira et al, 2017).…”

Biomarker responses of Nile Tilapia (Oreochromis niloticus) exposed to polluted water from Kelani river basin, Sri Lanka: Implications for biomonitoring river pollution

“…Erythrocytic micronuclei and nuclear abnormalities in fish are cellular level biomarkers for assessing genotoxic effects (Al-Sabti and Metcalfe 1995;Pathiratne 2016, 2017). The liver is a vital organ and analysis of liver histological alterations in fish can be used as a tissue level biomarker for assessing organ damage (Pathiratne et al 2010;Ghisi et al 2016;Kumar et al 2017).…”