Integrated Bioinformatic Analysis of Competing Endogenous RNA Network of Choriocarcinoma

Abstract

Background Numerous studies have demonstrated that noncoding RNAs are involved in choriocarcinoma (CC). The competing endogenous RNA (ceRNA) network plays an important role in the occurrence and development of carcinoma. However, the involvement of the ceRNA network in CC remains unclear. The current study aimed to investigate the regulatory mechanism of ceRNA in CC. Material/Methods We downloaded the messenger RNAs (mRNAs) expression profiles (GSE20510 and GSE65654) and microRNAs (miRNAs) expression profiles (GSE32346 and GSE130489) from GEO datasets. The limma package of R software was used to identify differentially expressed RNAs (DERNAs). Then, we performed functional annotation of the differentially expressed mRNAs (DEmRNAs). TargetScan, miRDB, miRWalk, and Starbase were used to construct a CC-specific ceRNA network and select key molecules. Results The results identified a total of 177 DEmRNAs and 189 differentially expressed miRNAs (DEmiRNAs) between the trophoblast and CC cell line samples. Ten differentially expressed lncRNAs (DElncRNAs) were obtained based on experimental studies. The DEmRNAs were mainly enriched in cell proliferation, positive regulation of the apoptotic process, and cell death. A total of 10 genes were ascertained as hub genes. Based on DEmRNAs, DEmiRNAs, and DElncRNAs, a CC-specific ceRNA network was established. Five DElncRNAs, 15 DEmiRNAs, and 45 DEmRNAs were identified. In addition, LINC00261, MEG3, MALAT1, H19, and OGFRP1 were identified as 5 key lncRNAs in choriocarcinoma. Conclusions This study provides novel insights into CC mechanisms and identified potential therapeutic targets for CC.

Long non-coding RNA (lncRNA) five prime to Xist (FTX) promotes retinoblastoma progression by regulating the microRNA-320a/with-no-lysine kinases 1 (WNK1) axis

“…The potential miRNA targets of FTX and downstream targets of miR-320a, as well as the putative binding sites were predicted by TargetScan ( http://www.targetscan.org/ ), and StarBase 3.0 ( http://starbase.sysu.edu.cn/ ), respectively [ 20 ].…”

Aberrantly Expressed lncRNA LINC00847 May Serve as a Promising Prognostic Factor for Thyroid Cancer

“…LncRNA LINC00847 has been confirmed to be involved in the occurrence and development of non-small cell lung cancer (NSCLC) [12], hepatocellular carcinoma [13], renal cell carcinoma [14] and laryngeal squamous cell carcinoma (LSCC) [15] in recent years. Notably, ln-cRNAs have been documented to target microRNAs (miRNAs) to exert biological effects on tumor progression, and miRNAs are often regarded as candidates for tumor progression, monitoring and treatment [16,17]. Based on online data, it is predicted that LINC00847 may target downstream miR-146b-5p.…”