Integrated approach for selecting efficient Saccharomyces cerevisiae for industrial lignocellulosic fermentations: Importance of yeast chassis linked to process conditions

Costa, Carlos E.; Romaní, Aloia; Cunha, Joana T.; Johansson, Börje; Domingues, Lucı́lia

doi:10.1016/j.biortech.2016.12.016

Cited by 70 publications

(67 citation statements)

References 49 publications

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“…cerevisiae strains; such as CCUGS3310-X, YRH388, YRH390, YRH392, YRH396, YRH400, YRH403, MT8-1/Xyl/BGL and MT8-1/Xyl showed less ethanol productivity. This result clearly confirmed that much of the xylose was converted to the xylitol (side-product) by these engineered yeast strains, which lowered the final yields of ethanol when compared with that of the wild-type strain SSA-1542 T [22,61–63]. The high xylose reductase (XR)/ xylitol dehydrogenase (XDH) activity ratios of the engineered yeast strains may contribute to a very high xylitol accumulation [64].…”

Section: Resultsmentioning

confidence: 60%

“…coipomoensis are associated with wood-feeding insects, which are able to ferment D-xylose to ethanol [19]. In addition, Candida shehatae , Pachysolen tannophilus , Pichia stipitis and some genetically engineered xylose-fermenting yeasts (including Saccharomyces cerevisiae ) have also been added in references [20–22]. However, these strains are relatively poor in their performance when applied as the fermenting yeasts in an industry processing [23].…”

Section: Introductionmentioning

confidence: 99%

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Screening and characterizing of xylanolytic and xylose-fermenting yeasts isolated from the wood-feeding termite, Reticulitermes chinensis

Ali

Xie

et al. 2017

PLoS ONE

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The effective fermentation of xylose remains an intractable challenge in bioethanol industry. The relevant xylanase enzyme is also in a high demand from industry for several biotechnological applications that inevitably in recent times led to many efforts for screening some novel microorganisms for better xylanase production and fermentation performance. Recently, it seems that wood-feeding termites can truly be considered as highly efficient natural bioreactors. The highly specialized gut systems of such insects are not yet fully realized, particularly, in xylose fermentation and xylanase production to advance industrial bioethanol technology as well as industrial applications of xylanases. A total of 92 strains from 18 yeast species were successfully isolated and identified from the gut of wood-feeding termite, Reticulitermes chinensis. Of these yeasts and strains, seven were identified for new species: Candida gotoi, Candida pseudorhagii, Hamamotoa lignophila, Meyerozyma guilliermondii, Sugiyamaella sp.1, Sugiyamaella sp. 2, and Sugiyamaella sp.3. Based on the phylogenetic and phenotypic characterization, the type strain of C. pseudorhagii sp. nov., which was originally designated strain SSA-1542T, was the most frequently occurred yeast from termite gut samples, showed the highly xylanolytic activity as well as D-xylose fermentation. The highest xylanase activity was recorded as 1.73 and 0.98 U/mL with xylan or D-xylose substrate, respectively, from SSA-1542T. Among xylanase-producing yeasts, four novel species were identified as D-xylose-fermenting yeasts, where the yeast, C. pseudorhagii SSA-1542T, showed the highest ethanol yield (0.31 g/g), ethanol productivity (0.31 g/L·h), and its fermentation efficiency (60.7%) in 48 h. Clearly, the symbiotic yeasts isolated from termite guts have demonstrated a competitive capability to produce xylanase and ferment xylose, suggesting that the wood-feeding termite gut is a promising reservoir for novel xylanases-producing and xylose-fermenting yeasts that are potentially valued for biorefinery industry.

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Section: Resultsmentioning

confidence: 60%

Section: Introductionmentioning

confidence: 99%

Screening and characterizing of xylanolytic and xylose-fermenting yeasts isolated from the wood-feeding termite, Reticulitermes chinensis

Ali

Xie

et al. 2017

PLoS ONE

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“…1A, B and C). It should be noted that xylitol is produced from xylose by the action of unspecific aldose reductases natively present on S. cerevisiae strains and that this production varies among yeast strains [5,16]. Thus, for analyzing xylan degrading capacity among different strains, the concentrations of xylose together with xylitol was considered ( Fig.…”

Section: Resultsmentioning

confidence: 99%

“…In addition, hemicellulosic CBP requires a highly engineered yeast, as xylose consumption pathways must be expressed together with xylan degrading enzymes. In this context, recent works have indicated that industrial isolates can present different intrinsic abilities to cope with genetic engineering strategies for xylose consumption [16] and expression of hydrolases [17,18]. Therefore, there is a need for a tailor-made development of hemicellulosic ethanol-producing yeast where intrinsic capabilities of host microorganism (suitable for the process) are previously selected.…”

Section: Introductionmentioning

confidence: 99%

Consolidated bioprocessing of corn cob-derived hemicellulose: engineered industrial Saccharomyces cerevisiae as efficient whole cell biocatalysts

Cunha

Romaní

Inokuma

et al. 2020

Preprint

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AbstractConsolidated bioprocessing, which combines saccharolytic and fermentative abilities in a single microorganism, is receiving increased attention to decrease environmental and economic costs in lignocellulosic biorefineries. Nevertheless, the economic viability of lignocellulosic ethanol is also dependent of an efficient utilization of the hemicellulosic fraction, which is mainly composed of xylose and may comprise up to 40 % of the total biomass. This major bottleneck is mainly due to the necessity of chemical/enzymatic treatments to hydrolyze hemicellulose into fermentable sugars and to the fact that xylose is not readily consumed by Saccharomyces cerevisiae – the most used organism for large-scale ethanol production. In this work, industrial S. cerevisiae strains, presenting robust traits such as thermotolerance and improved resistance to inhibitors, were evaluated as hosts for the cell-surface display of hemicellulolytic enzymes and optimized xylose assimilation, aiming at the development of whole-cell biocatalysts for consolidated bioprocessing of corn cob-derived hemicellulose. These modifications allowed the direct production of ethanol from non-detoxified hemicellulosic liquor obtained by hydrothermal pretreatment of corn cob, reaching an ethanol titer of 11.1 g/L corresponding to a yield of 0.328 gram per gram of potential xylose and glucose, without the need for external hydrolytic catalysts. Also, consolidated bioprocessing of pretreated corn cob was found to be more efficient for hemicellulosic ethanol production than simultaneous saccharification and fermentation with addition of commercial hemicellulases. These results show the potential of industrial S. cerevisiae strains for the design of whole-cell biocatalysts and paves the way for the development of more efficient consolidated bioprocesses for lignocellulosic biomass valorization, further decreasing environmental and economic costs.

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“…Acetic acid concentrations in various hydrothermally pretreated lignocellulosic biomass hydrolysates. All the values of acetic acid concentrations were normalized to the xylose concentrations (set as 20 g/L) in hydrolysates, respectively (*acetic acid in the pre‐hydrolysates after pretreatment; Costa, Romaní, Cunha, Johansson, & Domingues, ; Damay, Boboescu, Duret, Lalonde, & Lavoie, ; Demeke et al, ; Ko, Um, Woo, et al, ; Lu et al, ; Wei, Quarterman, Kim, Cate, & Jin, ; Zhou et al, )…”

Section: Introductionmentioning

confidence: 99%

Improved bioconversion of lignocellulosic biomass by Saccharomyces cerevisiae engineered for tolerance to acetic acid

Gong

et al. 2019

GCB Bioenergy

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Lignocellulosic biomass has considerable potential for the production of fuels and chemicals as a promising alternative to conventional fossil fuels. However, the bioconversion of lignocellulosic biomass to desired products must be improved to reach economic viability. One of the main technical hurdles is the presence of inhibitors in biomass hydrolysates, which hampers the bioconversion efficiency by biorefinery microbial platforms such as Saccharomyces cerevisiae in terms of both production yields and rates. In particular, acetic acid, a major inhibitor derived from lignocellulosic biomass, severely restrains the performance of engineered xylose‐utilizing S. cerevisiae strains, resulting in decreased cell growth, xylose utilization rate, and product yield. In this study, the robustness of XUSE, one of the best xylose‐utilizing strains, was improved for the efficient conversion of lignocellulosic biomass into bioethanol under the inhibitory condition of acetic acid stress. Through adaptive laboratory evolution, we successfully developed the evolved strain XUSAE57, which efficiently converted xylose to ethanol with high yields of 0.43–0.50 g ethanol/g xylose even under 2–5 g/L of acetic stress. XUSAE57 not only achieved twofold higher ethanol yields but also improved the xylose utilization rate by more than twofold compared to those of XUSE in the presence of 4 g/L of acetic acid. During fermentation of lignocellulosic hydrolysate, XUSAE57 simultaneously converted glucose and xylose with the highest ethanol yield reported to date (0.49 g ethanol/g sugars). This study demonstrates that the bioconversion of lignocellulosic biomass by an engineered strain could be significantly improved through adaptive laboratory evolution for acetate tolerance, which could help realize the development of an economically feasible lignocellulosic biorefinery to produce fuels and chemicals.

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Integrated approach for selecting efficient Saccharomyces cerevisiae for industrial lignocellulosic fermentations: Importance of yeast chassis linked to process conditions

Cited by 70 publications

References 49 publications

Screening and characterizing of xylanolytic and xylose-fermenting yeasts isolated from the wood-feeding termite, Reticulitermes chinensis

Screening and characterizing of xylanolytic and xylose-fermenting yeasts isolated from the wood-feeding termite, Reticulitermes chinensis

Consolidated bioprocessing of corn cob-derived hemicellulose: engineered industrial Saccharomyces cerevisiae as efficient whole cell biocatalysts

Improved bioconversion of lignocellulosic biomass by Saccharomyces cerevisiae engineered for tolerance to acetic acid

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