Integrated Analysis of Quantitative Proteome and Transcriptional Profiles Reveals the Dynamic Function of Maternally Expressed Proteins After Parthenogenetic Activation of Buffalo Oocyte

Abstract

Maternal-effect genes are especially critical for early embryonic development after fertilization and until massive activation of the embryonic genome occurs. By applying a tandem mass tag (TMT)-labeled quantitative proteomics combined with RNA sequencing approach, the proteome of the buffalo was quantitatively analyzed during parthenogenesis of mature oocytes and the two-cell stage embryo. Of 1908 quantified proteins, 123 differed significantly. The transcriptome was analyzed eight stages (GV, MII, 2-cell, 4-cell, 8-cell, 16-cell, morula, blastocyst) of Buffalo using the RNA sequencing approach, and a total of 3567 unique genes were identified to be differently expressed between all consecutive stages of pre-implantation development. Validation of proteomics results (TUBB3, CTNNA1, CDH3, MAP2K1), which are involved in tight junction and gap junction, revealing that the maternal expression of the proteins possibly plays a role in the formation of cellular junctions firstly after parthenogenetic activation. Correlation and hierarchical analyses of transcriptional profiles and the expression of NPM2 and NLRP5 mRNA of buffalo developed oocytes and parthenogenetic embryos indicated that the "maternal-to-zygotic transition" (MZT) process might exist in the model of parthenogenesis, which is similar to a normally fertilized embryo, and may occur between the 8-cell to 16-cell stage. These data provide a rich resource for further studies on maternal proteins and genes and are conducive to improving nuclear transfer technology.

Genomic Identification, Evolution, and Expression Analysis of Collagen Genes Family in Water Buffalo during Lactation

“…Remarkably, buffalo milk is more abundant in fat and protein than cow's milk [9], which has attracted widespread attention from the dairy industry. In the past several decades, numerous high-throughput data were generated and utilized for identifying the candidate genes related to traits of interest in buffalo, such as the potential genes related to milk or productive traits [10,11], transcriptome profiles of buffalo embryos with normal and retarded growth [12], and maternally expressed proteins in buffalo oocyte [13]. All these data, along with the complete buffalo genome sequence [14], provide the possibility to perform the gene family analysis at a genome-wide level.…”

Transcriptomic and proteomic analyses of the mechanisms of overwintering diapause in soybean pod borer (Leguminivora glycinivorella)

“…Proteins with fold-change < 0.83 or > 1.2 relative to the control, and false discovery rate (FDR)-corrected P-values < 0.05, were considered to be to be significantly altered. 33 DEP sequences were then subjected to BLAST searches against the UniProt database, and the top hits were selected using an Evalue cut-off of 0.001. Next, the top hits for each query sequence were retrieved and loaded into Blast2GO (version 3.3.5) to determine whether DEPs were significantly enriched in any functional subcategories.…”

Proteomics Analysis Reveals that Warburg Effect along with Modification in Lipid Metabolism Improves In Vitro Embryo Development under Low Oxygen

“…However, proteomics might provide us with a direct insight into the molecular mechanism governing embryo development, as proteins are the executors of most of the developmental programs. Moreover, proteomic analysis has been conducted to comprehend the mechanism of oocyte and embryo development in various species including buffalo [26,27], cows [28][29][30], mice [31][32][33][34][35][36] and pigs [37][38][39][40]. However, there is no study available determining the role of oxygen tension on in vitro embryo development at the proteome level.…”