Integrated Analysis of Proteomic and Transcriptomic Data Highlights Late Fetal Muscle Maturation Process

Voillet, Valentin; Cristobal, Magali San; Père, Marie-Christine; Billon, Yvon; Canario, Laurianne; Liaubet, Laurence; Lefaucheur, Louis

doi:10.1074/mcp.m116.066357

Cited by 23 publications

(25 citation statements)

References 80 publications

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“…Such a metabolic switch has also been recently reported in the metabolome of the amniotic fluid in humans between the second and the third trimesters of gestation [41]. The metabolic switch observed here is also consistent with our previous findings obtained with the same experimental design, which highlighted important variations in the muscle and intestinal transcriptomes and in the muscle and adipose tissue proteomes for a subset of the fetuses used in the present article [22,55,56,66].…”

Section: Discussionsupporting

confidence: 92%

“…However, its important role in polyamine synthesis during the pig gestation has previously been demonstrated [60]. As expected, the higher concentration of proline in MS compared to LW at 90 and 110 dg in plasma could be related to the lower maturity of LW piglet and to a potential delay in development already identified in those fetuses [22,55,56,66]. In addition, in plasma, crossbred fetuses have intermediate proline concentrations, with no specific maternal or paternal effect.…”

Section: Discussionmentioning

confidence: 56%

“…At birth, the piglets mainly rely on glycogen as an energy-yielding substrate before colostrum consumption [38,54]. Studying pig fetuses close to term in relation to their value for survival at birth, Leenhouwers et al [31] and Voillet et al [55] had shown that glycogen content in liver and muscle increased with chance of survival. Since glycogen is a multibranched polysaccharide of glucose described as reserve in tissues, it was unexpected to find it in the fluids that we studied (i.e., plasma, urine, and amniotic fluid).…”

Section: Discussionmentioning

confidence: 99%

“…The transcriptomic and/or proteomic analyses of the muscle, the intestine and subcutaneous adipose tissue underlined a switch in gene expressions between the two stages of gestation and a delay in the development of tissues and organs in LW piglets. Notably, genes involved in muscular development are up-regulated around 90 dg and genes linked to metabolic functions, like gluconeogenesis, are up-regulated at 110 dg either in muscle or adipose tissue whatever the genotype [22,55,56,66].…”

Section: Introductionmentioning

confidence: 99%

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The maturity in fetal pigs using a multi-fluid metabolomic approach

Lefort

Servien

Quesnel

et al. 2020

Preprint

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In mammalian species, the first days after birth are an important period for survival and the rates of mortality before weaning are high. In pigs, the perinatal deaths average 20% of the litter, with important economic and societal consequences. Among the factors influencing piglet survival at birth, the maturity is likely to be one of the most important. Maturity can be defined as the outcome of complex mechanisms of intra-uterine development and maturation occurring during the last month of gestation. Here, we provide new insights on maturity by studying the end of gestation at two different stages (three weeks before term and close to term) in two breeds of pigs that strongly differ in terms of neonatal survival. Since metabolomics is a promising approach for phenotype characterization or biomarker discovery, we provide a complete understanding of the metabolome of the fetuses in late gestation in three fluids (plasma, urine, and amniotic fluid). We found that biological processes related to amino acid and carbohydrate metabolisms are critical for piglet maturity. We also confirmed some previously described metabolites associated with delayed growth (e.g., proline and myo-inositol). Altogether, our study proposes new routes for a better characterization of piglet maturity at birth.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 56%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The maturity in fetal pigs using a multi-fluid metabolomic approach

Lefort

Servien

Quesnel

et al. 2020

Preprint

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“…The embryonic phase is important for skeletal muscle development and growth 18 . During this phase, the muscle fibres are formed and proliferated at early embryonic stage and hypertrophic at late stage 19,20 , which has great influence on production of meat mass 21 . In this paper, the protein profile of the embryonic skeletal muscle in sheep was established by using the TMT 22,23 and validated by PRM technology 24,25 .…”

Section: Discussionmentioning

confidence: 99%

Proteomic analyses of sheep (ovis aries) embryonic skeletal muscle

Wang

Shi

Zhao

et al. 2020

Sci Rep

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The growth and development of embryonic skeletal muscle plays a crucial role in sheep muscle mass. But proteomic analyses for embryonic skeletal development in sheep had been little involved in the past research. In this study, we explored differential abundance proteins during embryonic skeletal muscle development by the tandem mass tags (TMT) and performed a protein profile analyses in the longissimus dorsi of Chinese merino sheep at embryonic ages Day85 (D85N), Day105 (D105N) and Day135 (D135N). 5,520 proteins in sheep embryonic skeletal muscle were identified, and 1,316 of them were differential abundance (fold change ≥1.5 and p-value < 0.05). After the KEGG enrichment analyses, these differential abundance proteins were significant enriched in the protein binding, muscle contraction and energy metabolism pathways. After validation of the protein quantification with the parallel reaction monitoring (PRM), 41% (16/39) significant abundance proteins were validated, which was similar to the results of protein quantification with TMT. All results indicated that D85N to D105N was the stage of embryonic muscle fibers proliferation, while D105N to D135N was the stage of their hypertrophy. These findings provided a deeper understanding of the function and rules of proteins in different phases of sheep embryonic skeletal muscle growth and development.

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Integration analysis of transcriptome and proteome of Chinese Merino sheep (Ovis aries) embryonic skeletal muscle

Feng,

Hu,

Wang

et al. 2023

Animal Research and One Health

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The growth and development of sheep late fetal skeletal muscle undergoes significant changes. However, the specific mechanism remains unknown. In this study, we performed the comprehensive analysis of transcriptome and proteome of Chinese Merino sheep at embryonic ages Day85 (D85N), Day105 (D105N), and Day135 (D135N) by the tandem mass tags (TMT) and RNA‐seq methods. Totally 717, 1253, and 1873 differentially expressed genes (DEGs) were identified in the three comparison groups (D85N vs. D105N, D105N vs. D135N, and D85N vs. D135N). Among which 7, 80, and 162 DEGs were identified with the same trends at mRNA and protein levels in the three groups. Enrichment analysis showed that 7 genes with same trends in D85 vs. D105 have not been enriched in any pathways, which indicated that the development of skeletal muscle underwent significant changes with post‐transcription regulation during this period. These genes with same trends in D105N vs. D135N were mainly enriched in the pathways related to skeletal muscle metabolism and maturation, including oxidative phosphorylation, glycolysis/gluconeogenesis, tight junction, and HIF‐1 pathways, which indicated that the development of skeletal muscle tended to maturation during this period. These results provided evidence for ovine late fetal skeletal muscle fibers development from proliferating to thickening at simultaneous transcriptional and translational levels.

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Integrated Analysis of Proteomic and Transcriptomic Data Highlights Late Fetal Muscle Maturation Process

Cited by 23 publications

References 80 publications

The maturity in fetal pigs using a multi-fluid metabolomic approach

The maturity in fetal pigs using a multi-fluid metabolomic approach

Proteomic analyses of sheep (ovis aries) embryonic skeletal muscle

Integration analysis of transcriptome and proteome of Chinese Merino sheep (Ovis aries) embryonic skeletal muscle

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