Integrated analysis of lncRNA-miRNA-mRNA ceRNA network in human aortic dissection

Abstract

Background Many studies on long chain non-coding RNAs (lncRNAs) are published in recent years. But the roles of lncRNAs in aortic dissection (AD) are still unclear and should be further examined. The present work focused on determining the molecular mechanisms underlying lncRNAs regulation in aortic dissection on the basis of the lncRNA-miRNA-mRNA competing endogenous RNA (ceRNA) network. Methods This study collected the lncRNAs (GSE52093), mRNAs (GSE52093) and miRNAs (GSE92427) expression data within human tissue samples with aortic dissection group and normal group based on Gene Expression Omnibus (GEO) database. Results This study identified three differentially expressed lncRNAs (DELs), 19 differentially expressed miRNAs (DEmiRs) and 1046 differentially expressed mRNAs (DEGs) identified regarding aortic dissection. Furthermore, we constructed a lncRNA-miRNA-mRNA network through three lncRNAs (including two with up-regulation and one with down-regulation), five miRNAs (five with up-regulation), as well as 211 mRNAs (including 103 with up-regulation and 108 with down-regulation). Simultaneously, we conducted functional enrichment and pathway analyses on genes within the as-constructed ceRNA network. According to our PPI/ceRNA network and functional enrichment analysis results, four critical genes were found (E2F2, IGF1R, BDNF and PPP2R1B). In addition, E2F2 level was possibly modulated via lncRNA FAM87A-hsa-miR-31-5p/hsa-miR-7-5p or lncRNA C9orf106-hsa-miR-7-5p. The expression of IGF1R may be regulated by lncRNA FAM87A-hsa-miR-16-5p/hsa-miR-7-5p or lncRNA C9orf106-hsa-miR-7-5p. Conclusion In conclusion, the ceRNA interaction axis we identified is a potentially critical target for treating AD. Our results shed more lights on the possible pathogenic mechanism in AD using a lncRNA-associated ceRNA network.

Ribosome biogenesis in disease: new players and therapeutic targets

“…It has been shown that genetic differences in ribosome occupancy can affect protein translation. 296 , 297 A thorough understanding of the consequences of genetic differences among individuals is an important step toward personalized medicine. For the treatment of persons with a viral infection, the aim is to better understand the heterogeneity among individuals and determine who is most likely to benefit from a specific treatment.…”

Reliability and validity of the Chinese version of the biological rhythms interview of assessment in neuropsychiatry in patients with major depressive disorder

“…The MEQ and PSQI were used to test the criterion validity. Confirmatory factor analysis was performed to determine construct validity using the Statistical Product and Service Software Automatically tool (SPSSAU) (retrieved from https://www.spssau.com ), which has also been used in previous studies [ 30 – 32 ]. All other statistical analyses were conducted using SPSS version 23.0 (IBM Corp., Armonk, NY, USA).…”

Investigating the AC079305/DUSP1 Axis as Oxidative Stress-Related Signatures and Immune Infiltration Characteristics in Ischemic Stroke

“…For the sake of elucidating the underlying transcriptional regulation mechanisms among genes, an lncRNA-miRNA-mRNA triple ceRNA network was constructed in IS, as in previously reported similar methods [ 17 – 19 ]. According to the four online databases (miRcode, miRDB, miRTarBase, and TargetScan), we integrated lncRNA-miRNA pairs and miRNA-mRNA pairs into the ceRNA network, which was visualized by Cytoscape (v3.8.0) [ 20 ].…”