Integrated 3D conducting polymer-based bioelectronics for capture and release of circulating tumor cells

Hsiao, Yu-Sheng; Ho, Bo-Cheng; Yan, Hong-Xin; Kuo, Chien-Nan; Chueh, Di-Yen; Yu, Hsiao‐hua; Chen, Peilin

doi:10.1039/c5tb00096c

Cited by 47 publications

(51 citation statements)

References 41 publications

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“…The rapid developing cell printing and organs printing technology enables the production of complex biomimetic structures directly from computer-aided design models [114][115][116][117]. These techniques might eventually offer the opportunity for manufacturing bio-artificial organs to create functional substitutes for treating organs failure or dysfunctionality.…”

Section: Biochemical Analysis On 3d Printed Lab-on-a-chip Devicesmentioning

confidence: 99%

“…To obtain formidable and low-cost clinical therapeutic intervention platforms employed to monitor tumor progression and metathesis, fast rare cell isolation for circulating tumor cells (CTCs) genotyping to confirm the character of CTCs in tumor liquid biopsy was fairly significant. Hsiao et al created attractive bioelectronic interfaces (BEIs) based on conducting polymer with 3D structure [116]. Such structure could be incorporated on electronic devices for rare CTCs isolation, detection, and collection by means of an electrically caused cell release from chips with transfer printing technology.…”

Section: Cell Culture and Separationmentioning

confidence: 99%

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Chemical and biochemical analysis on lab-on-a-chip devices fabricated using three-dimensional printing

Zhang

2016

TrAC Trends in Analytical Chemistry

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Section: Biochemical Analysis On 3d Printed Lab-on-a-chip Devicesmentioning

confidence: 99%

Section: Cell Culture and Separationmentioning

confidence: 99%

Chemical and biochemical analysis on lab-on-a-chip devices fabricated using three-dimensional printing

Zhang

2016

TrAC Trends in Analytical Chemistry

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“…The key concept for the capture and release of the CTCs was the dynamic control of the Pll-g-PEG-biotin coating on the PEDOTAc nanorods. The function of the device was demonstrated by capturing of human breast adenocarcinoma cells, which subsequently could be released by repeated potential sweeping between -0.8 to 0.5 V [144].…”

Section: Cell Biologymentioning

confidence: 99%

Organic Bioelectronic Tools for Biomedical Applications

2015

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Abstract:Organic bioelectronics forms the basis of conductive polymer tools with great potential for application in biomedical science and medicine. It is a rapidly growing field of both academic and industrial interest since conductive polymers bridge the gap between electronics and biology by being electronically and ionically conductive. This feature can be employed in numerous ways by choosing the right polyelectrolyte system and tuning its properties towards the intended application. This review highlights how active organic bioelectronic surfaces can be used to control cell attachment and release as well as to trigger cell signaling by means of electrical, chemical or mechanical actuation. Furthermore, we report on the unique properties of conductive polymers that make them outstanding materials for labeled or label-free biosensors. Techniques for electronically controlled ion transport in organic bioelectronic devices are introduced, and examples are provided to illustrate their use in self-regulated medical devices. Organic bioelectronics have great potential to become a primary platform in future bioelectronics. We therefore introduce current applications that will aid in the development of advanced in vitro systems for biomedical science and of automated systems for applications in neuroscience, cell biology and infection biology. Considering this broad spectrum of applications, organic bioelectronics could lead to timely detection of disease, and facilitate the use of remote and personalized medicine. As such, organic bioelectronics might contribute to efficient healthcare and reduced hospitalization times for patients. OPEN ACCESSElectronics 2015, 4 880

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“…We have previously shown that a variety of nanotopographies of varying size and morphology can be engineered using PEDOT by simply changing the electropolymerization voltage or temperature . These scientific developments have led to applications of PEDOT‐materials for cancer therapeutics . A vast majority of studies conducted to understand the cancer cell behavior employ inorganic materials.…”

Section: Introductionmentioning

confidence: 99%

Mechanotactic Activation of TGF‐β by PEDOT Artificial Microenvironments Triggers Epithelial to Mesenchymal Transition

et al. 2020

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Epithelial to mesenchymal transition (EMT) is integral for cells to acquire metastatic properties, and ample evidence links it to bioorganic framework of the tumor microenvironment (TME). Hydroxymethyl‐functionalized 3,4‐ethylenedioxythiophene polymer (PEDOT‐OH) enables construction of diverse nanotopography size and morphologies and is therefore exploited to engineer organic artificial microenvironments bearing nanodots from 300 to 1000 nm in diameter to understand spatiotemporal EMT regulation by biophysical components of the TME. MCF‐7 breast cancer cells are cultured on these artificial microenvironments, and temporal regulation of cellular morphology and EMT markers is investigated. The results show that upon physical stimulation, cells on 300 nm artificial microenvironments advance to EMT and display a decreased extracellular matrix (ECM) protein secretion. In contrast, cells on 500 nm artificial microenvironments are trapped in EMT‐imbalance. Interestingly, cells on 1000 nm artificial microenvironments resemble those on control surfaces. Upon further investigation, it is found that EMT induction is triggered via transforming growth factor β (TGF‐β) and ECM cleaving protein, matrix metalloproteinease‐9. Immunostaining EMT proteins highlighted that EMT induction is achieved through attenuation of cell–cell and cell–microenvironment adhesions. The physical stimulation‐induced TGF‐β perturbation can have a profound impact on the understanding of tumor‐promoting signaling cascades originated by cellular microenvironment.

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Integrated 3D conducting polymer-based bioelectronics for capture and release of circulating tumor cells

Abstract: 3D conducting polymer-based bioelectronic interface (BEI) devices for dynamically controlling circulating tumor cell capture/release performance through the cyclic potential of electrical stimulation.

Cited by 47 publications

References 41 publications

Chemical and biochemical analysis on lab-on-a-chip devices fabricated using three-dimensional printing

Chemical and biochemical analysis on lab-on-a-chip devices fabricated using three-dimensional printing

Organic Bioelectronic Tools for Biomedical Applications

Mechanotactic Activation of TGF‐β by PEDOT Artificial Microenvironments Triggers Epithelial to Mesenchymal Transition

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