Integrated 16S rRNA sequencing and nontargeted metabolomics analysis to reveal the mechanisms of Yu-Ye Tang on type 2 diabetes mellitus rats

Abstract

IntroductionYu–Ye Tang (YYT) is a classical formula widely used in treatment of type 2 diabetes mellitus (T2DM). However, the specific mechanism of YYT in treating T2DM is not clear.MethodsThe aim of this study was to investigate the therapeutic effect of YYT on T2DM by establishing a rat model of T2DM. The mechanism of action of YYT was also explored through investigating gut microbiota and serum metabolites.ResultsThe results indicated YYT had significant therapeutic effects on T2DM. Moreover, YYT could increase the abundance of Lactobacillus, Candidatus_Saccharimonas, UCG-005, Bacteroides and Blautia while decrease the abundance of and Allobaculum and Desulfovibrio in gut microbiota of T2DM rats. Nontargeted metabolomics analysis showed YYT treatment could regulate arachidonic acid metabolism, alanine, aspartate and glutamate metabolism, arginine and proline metabolism, glycerophospholipid metabolism, pentose and glucuronate interconversions, phenylalanine metabolism, steroid hormone biosynthesis, terpenoid backbone biosynthesis, tryptophan metabolism, and tyrosine metabolism in T2DM rats.DiscussionIn conclusion, our research showed that YYT has a wide range of therapeutic effects on T2DM rats, including antioxidative and anti-inflammatory effects. Furthermore, YYT corrected the altered gut microbiota and serum metabolites in T2DM rats. This study suggests that YYT may have a therapeutic impact on T2DM by regulating gut microbiota and modulating tryptophan and glycerophospholipid metabolism, which are potential key pathways in treating T2DM.

Investigation of the therapeutic effect of Hedan tablets on high‐fat diet‐induced obesity in rats by GC–MS technology and 16S ribosomal RNA gene sequencing

“…Furthermore, research (Li et al, 2020) has shown that a mixture of Lactobacilli species can modulate the gut microbiota in mice fed a high‐fat diet, resulting in the increased production of SCFAs (such as acetic acid and butyric acid), thereby regulating lipid metabolism in adipose tissue and mitigating hepatic lipid accumulation to prevent obesity. Candidatus Saccharimonas , an acid‐producing bacterium, has been reported to possess significant potential in ameliorating host metabolic disorders, inflammation, and immune response (Luo et al, 2022; Ma et al, 2023). The Prevotella genus primarily participates in carbohydrate and monosaccharide metabolism, leading to the production of SCFAs that confer beneficial effects on intestinal health (Atzeni et al, 2021; Zhao et al, 2021).…”

Bibliometric analysis of global research trends between gut microbiota and pancreatic cancer: from 2004 to 2023

“…The gut microbiota (GM) is an enormous and diverse community containing trillions of species of bacteria, fungi, and viruses, the most important of which are enteric bacteria that parasitize the intestine and can help their hosts perform a variety of physiological functions ( Li et al, 2016 ; Liu et al, 2021 ). According to statistics, the human gut is home to over 1,000 different species of bacteria ( Ma et al, 2023 ). With the advances in targeted 16S rRNA pyrophosphate sequencing and metagenomics sequencing, an increasing number of studies have revealed that GM is closely linked to human diseases such as diabetes, cardiovascular diseases, autism, rheumatoid arthritis, and tumors ( Hou et al, 2021 ; Settanni et al, 2021 ; Xu et al, 2021 ; Zaiss et al, 2021 ; Zhao et al, 2022 ; Feng et al, 2023 ; Ma et al, 2023 ).…”

“…According to statistics, the human gut is home to over 1,000 different species of bacteria ( Ma et al, 2023 ). With the advances in targeted 16S rRNA pyrophosphate sequencing and metagenomics sequencing, an increasing number of studies have revealed that GM is closely linked to human diseases such as diabetes, cardiovascular diseases, autism, rheumatoid arthritis, and tumors ( Hou et al, 2021 ; Settanni et al, 2021 ; Xu et al, 2021 ; Zaiss et al, 2021 ; Zhao et al, 2022 ; Feng et al, 2023 ; Ma et al, 2023 ). Notably, recent studies have pointed out a substantial link between PC and GM.…”