Integrated 16S rRNA gene sequencing and LC/MS-based metabolomics ascertained synergistic influences of the combination of acupuncture and NaoMaiTong on ischemic stroke

Xian, Minghua; Lin, Senjie; Zhan, Sikai; Chen, Shaoru; Lin, Huiting; Cai, Jiale; Hu, Tao; Wang, Shumei

doi:10.1016/j.jep.2022.115281

Cited by 18 publications

(19 citation statements)

References 52 publications

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“…Firmicutes are the main bacterial phylum in the mammalian gut. Moreover, the abundance of Firmicutes decreased in patients with post-stroke cognitive impairment ( Ling et al, 2020 ) and in MCAO pigs ( Jeon et al, 2020 ), rats ( Xian et al, 2022 ), and mice ( Gao et al, 2021 ). Firmicutes showed a negative correlation with lesion volume, midline shift, and hemorrhage volume in ischemic stroke ( Jeon et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Angong Niuhuang Pill ameliorates cerebral ischemia/reperfusion injury in mice partly by restoring gut microbiota dysbiosis

et al. 2022

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Section: Discussionmentioning

confidence: 99%

Angong Niuhuang Pill ameliorates cerebral ischemia/reperfusion injury in mice partly by restoring gut microbiota dysbiosis

et al. 2022

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“…An imbalance in intestinal flora can lead to increased phenylalanine and isoleucine in the peripheral accumulation, which can promote inflammation ( Wang et al., 2019 ). Disturbance of the intestinal flora is associated with the severity of ischemic stroke, and it also may be closely related to metabolism and inflammation in the host ( Xian et al., 2022 ). It was discovered in this study that Prevotellaceae was significantly positively correlated with IL-6; Lactobacillus intestinalis was negative interrelated to IL-1β and L-phenylalanine.…”

Section: Discussionmentioning

confidence: 99%

“…Metabolomics has been used to evaluate the physiological state of the body because of its integrity ( Liu et al., 2022 ). Metabolomics is necessary to discover the interconnection between intestinal flora metabolites and changes that occur during CIRI to further characterize disease progression and identify disease-related biomarkers ( Xian et al., 2022 ). The research on metabolomics was in possession of dynamic, integral, and systematic characteristics.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive evaluation of the mechanism of Gastrodia elata Blume in ameliorating cerebral ischemia–reperfusion injury based on integrating fecal metabonomics and 16S rDNA sequencing

Ding

Liu

et al. 2022

Front. Cell. Infect. Microbiol.

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Gastrodia elata Blume was used to treat stroke and headaches caused by “Feng” for thousands of years. The present study has shown a significant effect of G. elata Blume in improving cerebral ischemia–reperfusion injury (CIRI). However, the mechanism of G. elata Blume in improving CIRI by regulating the intestinal flora has not been reported until now. This research aimed to comprehensively evaluate the mechanism of G. elata Blume in CIRI based on fecal metabolomics and 16S rDNA sequencing. The rat model with CIRI was created based on the Zea Longa method. Enzyme-linked immunosorbent assay (ELISA) was used to monitor the inflammatory factors in rat serum. Damages of brain tissues were observed using hematoxylin and eosin (H&E) staining. Cerebral infarction was observed by 2,3,5-triphenyltetrazolium chloride (TTC) staining. The balance of intestinal flora in cecal contents of rats was evaluated by high-throughput sequencing. Changes of metabolites in the intestinal flora were evaluated by fecal metabolomics through Ultra high performance liquid chromatography-orbitrap exploris-mass spectrometer (UHPLC-OE-MS). The area of brain necrosis, cerebral infarction volume, and the contents of inflammatory factors in CIRI rats can be effectively reduced after oral administration of G. elata Blume. CIRI can cause disturbances in the intestinal flora and its associated metabolites. G. elata Blume can significantly regulate the composition of the intestinal microflora. It reversed CIRI-induced changes in the levels of multiple intestinal bacteria, including Prevotellaceae, Coriobacteriaceae; Prevotella, Gamma proteobacteria unclassified, Barnesiella, Escherichia, Shigella; uncultured Shigella sp., Flavonifractor sp., Escherichia sp. enrichment culture clone NBAR004, Veillonella sp. R-32, and Lactobacillus intestinalis. The levels of metabolites in cecal contents were disturbed in rats with CIRI, including amino acid, purine, and sphingolipid metabolism. The changes in the level of biomarkers in amino acid metabolism induced by CIRI were significantly reversed after treatment with G. elata Blume. Correlation studies show that Prevotellaceae was significantly positively correlated with interleukin (IL)-6, and L. intestinalis and L-phenylalanine were negatively interrelated to IL-1β. Beta-glycerophosphoric acid was significantly negatively interrelated to high-sensitivity C-reactive protein (hs-CRP). There were significantly negative correlations between L-phenylalanine and L. intestinalis, beta-glycerophosphoric acid and Prevotellaceae. G. elata Blume protected against CIRI, which may be related to improved intestinal microflora composition and metabolism, resulting in decreased inflammation.

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“…The mechanism of action of acupuncture might be associated with its effects on intestinal microecology and plasma metabolism. It might influence 10.3389/fnins.2023.1158057 Turicibacter, isoflavones, phytoestrogen metabolites (Xian et al, 2022), andIPA levels (Li et al, 2022). Additionally, the combination of acupuncture and TCM might have synergistic effects, which might further enhance the recovery of IS when administered together.…”

Section: Tcm Prescriptionmentioning

confidence: 99%

Interventional strategies for ischemic stroke based on the modulation of the gut microbiota

Wang

Liu

2023

Front. Neurosci.

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The microbiota-gut-brain axis connects the brain and the gut in a bidirectional manner. The organism’s homeostasis is disrupted during an ischemic stroke (IS). Cerebral ischemia affects the intestinal flora and microbiota metabolites. Microbiome dysbiosis, on the other hand, exacerbates the severity of IS outcomes by inducing systemic inflammation. Some studies have recently provided novel insights into the pathogenesis, efficacy, prognosis, and treatment-related adverse events of the gut microbiome in IS. In this review, we discussed the view that the gut microbiome is of clinical value in personalized therapeutic regimens for IS. Based on recent non-clinical and clinical studies on stroke, we discussed new therapeutic strategies that might be developed by modulating gut bacterial flora. These strategies include dietary intervention, fecal microbiota transplantation, probiotics, antibiotics, traditional Chinese medication, and gut-derived stem cell transplantation. Although the gut microbiota-targeted intervention is optimistic, some issues need to be addressed before clinical translation. These issues include a deeper understanding of the potential underlying mechanisms, conducting larger longitudinal cohort studies on the gut microbiome and host responses with multiple layers of data, developing standardized protocols for conducting and reporting clinical analyses, and performing a clinical assessment of multiple large-scale IS cohorts. In this review, we presented certain opportunities and challenges that might be considered for developing effective strategies by manipulating the gut microbiome to improve the treatment and prevention of ischemic stroke.

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Integrated 16S rRNA gene sequencing and LC/MS-based metabolomics ascertained synergistic influences of the combination of acupuncture and NaoMaiTong on ischemic stroke

Cited by 18 publications

References 52 publications

Angong Niuhuang Pill ameliorates cerebral ischemia/reperfusion injury in mice partly by restoring gut microbiota dysbiosis

Angong Niuhuang Pill ameliorates cerebral ischemia/reperfusion injury in mice partly by restoring gut microbiota dysbiosis

Comprehensive evaluation of the mechanism of Gastrodia elata Blume in ameliorating cerebral ischemia–reperfusion injury based on integrating fecal metabonomics and 16S rDNA sequencing

Interventional strategies for ischemic stroke based on the modulation of the gut microbiota

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