Intake of stigmasterol and β-sitosterol alters lipid metabolism and alleviates NAFLD in mice fed a high-fat western-style diet

Feng, Simin; Dai, Zhuqing; Liu, Anna B.; Huang, Jinbao; Narsipur, Nihal; Guo, Grace L.; Kong, Bo; Reuhl, Kenneth R.; Lu, Wenyun; Luo, Zisheng; Yang, Chung S.

doi:10.1016/j.bbalip.2018.08.004

Cited by 142 publications

(93 citation statements)

References 46 publications

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“…The ability of the rat IM to make SF from GRP changes with the length of dietary exposure to broccoli [11]. Whereas it is known that broccoli consumption also affects the ability of many human IM to produce SF, it is well established that there is significant variation among individuals in the response of their IM to broccoli consumption [34]. For example, this might be due to absence of certain microbiota in some individuals, microbiota that enhance or disrupt SF formation and absorption.…”

Section: Discussionmentioning

confidence: 99%

Biomarkers of Broccoli Consumption: Implications for Glutathione Metabolism and Liver Health

et al. 2020

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Diet and lifestyle choices contribute to obesity and liver disease. Broccoli, a brassica vegetable, may mitigate negative effects of both diet and lifestyle. Currently, there are no clinically relevant, established molecular biomarkers that reflect variability in human absorption of brassica bioactives, which may be the cause of variability/inconsistencies in health benefits in the human population. Here, we focused on the plasma metabolite profile and composition of the gut microbiome in rats, a relatively homogenous population in terms of gut microbiota, genetics, sex and diet, to determine if changes in the plasma metabolite profiles caused by dietary broccoli relate to molecular changes in liver. Our aim was to identify plasma indicators that reflect how liver health is impacted by dietary broccoli. Rats were fed a 10% broccoli diet for 14 days. We examined the plasma metabolite composition by metabolomics analysis using GC–MS and gut microbiota using 16S sequencing after 0, 1, 2, 4, 7, 14 days of broccoli feeding. We identified 25 plasma metabolites that changed with broccoli consumption, including metabolites associated with hepatic glutathione synthesis, and with de novo fatty acid synthesis. Glutamine, stearic acid, and S-methyl-L-cysteine (SMC) relative abundance changes correlated with changes in gut bacteria previously implicated in metabolic disease and with validated increases in expression of hepatic NAD(P)H dehydrogenase [quinone] 1 (NQO1) and nuclear factor (erythroid-derived 2)-like 2 (Nrf2), associated with elevated hepatic glutathione synthesis. Circulating biomarkers following broccoli consumption reflect gut–liver axis health.

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Section: Discussionmentioning

confidence: 99%

Biomarkers of Broccoli Consumption: Implications for Glutathione Metabolism and Liver Health

et al. 2020

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“…Constant consumption of stigmasterol can reduce blood LDL cholesterol by 8%-10%, which lowers the risk of cardiovascular disease [9]. Further, stigmasterol inhibits the accumulation of fatty acids [10]. In plant seeds, stigmasterol promotes cell proliferation, maturation, and germination [11].…”

Section: Introductionmentioning

confidence: 99%

Stigmasterol Causes Ovarian Cancer Cell Apoptosis by Inducing Endoplasmic Reticulum and Mitochondrial Dysfunction

2020

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Background: Phytosterols have physiological effects and are used as medicines or food supplements. Stigmasterol has shown anticancer effects against various cancers such as hepatoma, cholangiocarcinoma, gall bladder carcinoma, endometrial adenocarcinoma and skin, gastric, breast, prostate, and cervical cancer. However, there are no reports on stigmasterol’s effects on ovarian cancer. Methods: We investigated the effects of stigmasterol on proapoptotic signals, mitochondrial function, reactive oxygen species production, and the cytosolic and mitochondrial calcium levels in human ovarian cancer cells, to understand the mechanisms underlying the effects of stigmasterol on ovarian cancer cells. We also conducted migration assay to confirm whether that stigmasterol inhibits ovarian cancer cell migration. Results: Stigmasterol inhibited development of human ovarian cancer cells. However, it induced cell apoptosis, ROS production, and calcium overload in ES2 and OV90 cells. In addition, stigmasterol stimulated cell death by activating the ER-mitochondrial axis. We confirmed that stigmasterol suppressed cell migration and angiogenesis genes in human ovarian cancer cells. Conclusions: Our findings suggest that stigmasterol can be used as a new treatment for ovarian cancer.

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“…It is worth mentioning that stigmasterol is related to almost the same genes as C09367, excepting the AR gene. Mouse experiments demonstrated that stigmasterol prevented the HFWD-induced elevation of some di-and triacylglycerol species, decreased serum levels of ceramides, inhibited intestinal absorption of cholesterol and plant sterol, and suppressed hepatic cholesterol [35,36]. Therefore, these key ingredients can achieve a good effect on CHD by lowering cholesterol, inhibiting platelet aggregation, suppressing in ammatory response and relieving the pain.…”

Section: Discussionmentioning

confidence: 99%

A Network Pharmacology-Based Study on Active Ingredients of Corydalis Rhizoma on Coronary Heart Disease

Liu

Wei

Zhuang

et al. 2020

Preprint

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Background. Corydalis Rhizoma(CR) showed a high efficacy for coronary heart disease (CHD). However, the interaction between the active ingredients of CR and the targets of CHD has not been unequivocally explained in previous researches. To study the active components and potential targets of Corydalis Rhizoma and to determine the mechanism underlying the exact effect of Corydalis Rhizoma on coronary heart disease, a method of network pharmacology was used. Materials and Methods. The active components of CR and targets corresponding to each component were scanned out from Traditional Chinese medicine systems pharmacology database and analysis platform (TCMSP), and target genes of CHD were searched on GeneCards database and Online Mendelian Inheritance in Man(OMIM) database. The active components and common targets of CR and CHD were used to build the “CR-CHD” network through Cytoscape (version 3.2.1) software as well as protein-protein interaction(PPI) network on String database. Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment analysis was executed by clusterProfiler(version 3.8) and DOSE(version 3.6) package on R platform. Results. 49 active ingredients and 394 relevant targets of CR and the 7173 CHD-related genes were retrieved. 40 common genes were selected for subsequent analysis. Crucial biological processes and pathways were obtained and analyzed, including DNA-binding transcription activator activity, RNA polymerase II-specific, RNA polymerase II transcription factor binding, kinase regulator activity, ubiquitin-like protein ligase binding, fluid shear stress and atherosclerosis, TNF signaling pathway, apoptosis, MAPK signaling pathway and PI3K-Akt signaling pathway. Conclusions. Overall, CR could alleviate CHD through the mechanisms predicted by network pharmacology, laying the foundation for future development of new drugs from traditional Chinese medicine on CHD.

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Intake of stigmasterol and β-sitosterol alters lipid metabolism and alleviates NAFLD in mice fed a high-fat western-style diet

Cited by 142 publications

References 46 publications

Biomarkers of Broccoli Consumption: Implications for Glutathione Metabolism and Liver Health

Biomarkers of Broccoli Consumption: Implications for Glutathione Metabolism and Liver Health

Stigmasterol Causes Ovarian Cancer Cell Apoptosis by Inducing Endoplasmic Reticulum and Mitochondrial Dysfunction

A Network Pharmacology-Based Study on Active Ingredients of Corydalis Rhizoma on Coronary Heart Disease

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