Intact mass analysis of monoclonal antibodies by capillary electrophoresis—Mass spectrometry

Top-down proteomic analysis of protein pharmaceuticals, mixtures of protein complexes, and ribosomal protein extracts by capillary zone electrophoresis-mass spectrometry

“…A trace quantity of the mAb is thereby composed of light chain domains bound via non-covalent interactions instead of disulfide bonds. The presence of such species has been reported previously, and such fragments are known to occur under native conditions [2,[286][287][288].…”

“…Analysis of intact mAbs is typically performed in order to determine protein molecular mass, extent of oligomerization, and additional structural information, such as the presence of free chains [266], glycosylation [1, 25,267], as well as various PTM combinations [1,268]. On conventional Orbitrap™ instruments, MS resolution is inversely proportional to the square root of m/z, which makes baseline resolution of higher molecular weight species challenging.…”

On‐line capillary electrophoresis/laser‐induced fluorescence/mass spectrometry analysis of glycans labeled with Teal™ fluorescent dye using an electrokinetic sheath liquid pump‐based nanospray ion source

“…Although high reactivity and separation efficiency of the Teal™ dye in CE/LIF assays have been reported earlier, MS detection of Teal‐labeled glycans is highly desirable to generate accurate identification and minimize peak assignment ambiguity. The CE/MS method that we adopted for the analysis of Teal‐labeled glycans uses an electrokinetically pumped nanospray sheath liquid CE/MS interface design (Figure S1, supporting information) . The analytes are injected into the separation capillary from the capillary inlet, which is placed inside the CE system.…”

Recent advances in CE‐MS coupling: Instrumentation, methodology, and applications

“…Their applications were further extensively demonstrated especially in the field of proteomics . The commercialized version of this arrangement with the trademark EMASS‐II (Prince Technologies, Netherlands) was used by several groups for characterization of antibodies and polysaccharides . A flow‐through microvial interface, recently used for investigation of protein glycosylation, is based on the very same principal .…”