Intact Articular Cartilage Cryopreservation In Vivo Evaluation

Marco, Fernando; León, Carlos; López-Oliva, F.; Perez, Anselmo José; A, Sanchez-Barba; López-Durán, L.

doi:10.1097/00003086-199210000-00003

Cited by 32 publications

(32 citation statements)

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“…Although, the press-fit technique is commonly used in autograft plug transplantation, it is used rarely for allografts. However, press-fit implantation of osteochondral allografts has been researched in animal models [2,35,38,43,48]. Graft and host cartilage remain distinct in animal models despite press-fit implantation techniques [2,29,48].…”

Section: Discussionmentioning

confidence: 99%

“…Half of the 48 OCPs were fresh osteochondral autografts, harvested from the non-load-bearing anterior region of the medial femoral condyles immediately before implantation; the other 24 grafts were allograft plugs harvested from the load-bearing region of the medial femoral condyles of 4 age-matched donor sheep. Allografts were cryopreserved [35] in a 15% glycerol medium by slow-freezing and stored at -70 "C up to 72 h prior to implantation. OCPs were implanted press-fit; 6 mm plugs were placed into 5 mm defects.…”

Section: Methodsmentioning

confidence: 99%

“…Unfortunately, the limited availability of fresh articular cartilage makes tissue banking desirable. Cryopreservation increases osteochondral allograft availability while partially retaining chondrocyte metabolic function and viability [35,41].…”

Section: Introductionmentioning

confidence: 99%

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Chondrocyte viability in press‐fit cryopreserved osteochondral allografts

Gole

Poulsen

Marzo

et al. 2004

Journal Orthopaedic Research

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The viability of chondrocytes in press-fit glycerol-preserved osteochondral allografts was compared to that in fresh autografts, after transplantation into load-bearing and non-load-bearing sites in mature sheep stifle joints. We used macroscopic grading, tonometer pen indentation testing, histology, sulfate uptake and viability as determined by confocal-microscopy to assess cartilage condition. Despite there being no statistical differences between macroscopic appearance and tonometer testing of all grafts, confocal microscopy and histology demonstrated a positive effect of load-bearing placement on cryopreserved osteochondral allografts. Allografts transplanted into load-bearing sites demonstrated superior confocal microscopy-measured chondrocyte viability (77% ? 17%1sD) than those transplanted into non-load-bearing sites (25% f 2%). Load-bearing effect was not seen in autografts (78% k ISXI), and was comparable in adjacent cartilage (830/0 5 9%). Similarly, load-bearing allografts demonstrated histological scoring closer to that of autografts and adjacent cartilage, all of which fared significantly better than non-load-bearing allografts. Load-bearing allografts had a greater amount of fibrocartilage than autografts or adjacent cartilage but less fibrocartilage than nonload-bearing allografts. Both autografts and allografts had non-significant increases in metabolism compared to adjacent cartilage as measured by sulfate-uptake. Load-bearing placement improved chondrocyte viability of glycerol cryopreserved osteochondral allograft following a press-fit implantation. Published by Elsevier Ltd. on behalf of orthopaedic Research Society.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Chondrocyte viability in press‐fit cryopreserved osteochondral allografts

Gole

Poulsen

Marzo

et al. 2004

Journal Orthopaedic Research

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“…Studies with fresh autogeneous cartilage also demonstrated that the re-implanted cartilage appeared grossly normal and smooth [4,15] and the Safranin-O staining intensity of the implanted cartilage decreased [11],but these grafts showed some deterioration in the quantitative scale of cartilage degradation [18]. On the other hand, one study with cryopreserved autogeneous cartilage demonstrated that the re-implanted cartilage had fewer living chondrocytes and decreased safranin-O staining [4].…”

Section: 【Discussion】mentioning

confidence: 99%

“…Preserved chondrocytes of allografts showed a prominent rough endoplasmic reticulum and sub-spherical nuclei upon ultrastructural observation [6,18,30]. On the other hand, degenerated chondrocytes had electron dense cytoplasm and irregular nuclei [6,14].…”

Section: 【Discussion】mentioning

confidence: 99%

Vitreous preservation of articular cartilage from cryoinjury in rabbits

et al. 2012

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【Abstract】Frozen osteoarticular grafts treated with liquid nitrogen are utilized for joint reconstruction after tumor resection, but the joints may subsequently develop osteoarthritic changes. To preserve articular cartilage from cryoinjury, we modified a vitrification method utilized for embryo cryopreservation and demonstrated in vitro that our vitrification protocol was effective for protecting cartilage from cryoinjury. In this study, we investigated in vivo whether this vitrification method could protect against

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Thermoreversible hydrogel scaffolds for articular cartilage engineering

Fisher¹,

Jo²,

Mikos³

et al. 2004

J Biomedical Materials Res

116

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Articular cartilage has limited potential for repair. Current clinical treatments for articular cartilage damage often result in fibrocartilage and are associated with joint pain and stiffness. To address these concerns, researchers have turned to the engineering of cartilage grafts. Tissue engineering, an emerging field for the functional restoration of articular cartilage and other tissues, is based on the utilization of morphogens, scaffolds, and responding progenitor/stem cells. Because articular cartilage is a water-laden tissue and contains within its matrix hydrophilic proteoglycans, an engineered cartilage graft may be based on synthetic hydrogels to mimic these properties. To this end, we have developed a polymer system based on the hydrophilic copolymer poly(propylene fumarate-co-ethylene glycol) [P(PF-co-EG)]. Solutions of this polymer are liquid below 25°C and gel above 35°C, allowing an aqueous solution containing cells at room temperature to form a hydrogel with encapsulated cells at physiological body temperature. The objective of this work was to determine the effects of the hydrogel components on the phenotype of encapsulated chondrocytes. Bovine articular chondrocytes were used as an experimental model. Results demonstrated that the components required for hydrogel fabrication did not significantly reduce the proteoglycan synthesis of chondrocytes, a phenotypic marker of chondrocyte function. In addition, chondrocyte viability, proteoglycan synthesis, and type II collagen synthesis within P(PF-co-EG) hydrogels were investigated. The addition of bone morphogenetic protein-7 increased chondrocyte proliferation with the P(PF-co-EG) hydrogels, but did not increase proteoglycan synthesis by the chondrocytes. These results indicate that the temperatureresponsive P(PF-co-EG) hydrogels are suitable for chondrocyte delivery for articular cartilage repair.

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Intact Articular Cartilage Cryopreservation In Vivo Evaluation

Cited by 32 publications

References 0 publications

Chondrocyte viability in press‐fit cryopreserved osteochondral allografts

Chondrocyte viability in press‐fit cryopreserved osteochondral allografts

Vitreous preservation of articular cartilage from cryoinjury in rabbits

Thermoreversible hydrogel scaffolds for articular cartilage engineering

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