Insulin stimulates gene expression of ferritin light chain in osteoblast cells

Han, Su Hee; Saminathan, Sethuraman Odathurai; Kim, Sungjin

doi:10.1002/jcb.22879

Cited by 11 publications

(5 citation statements)

References 55 publications

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“…In particular, Ftl and Ezr represent two single peptide‐based protein identifications and their validation provides a strong support to the whole proteomic analysis. Moreover, concerning Ftl, it has been reported that insulin may control iron metabolism by stimulating gene and protein expression of Ftl in osteoblast cells . It is well‐known that insulin acts as tissue growth factor as well as hormone, sharing with IGF‐1 part of the same intracellular signaling with different affinity to IGF‐1R .…”

Section: Proteins Identified As Single Speciesmentioning

confidence: 99%

Insulin‐like growth factor 1 receptor signaling induced by supraphysiological doses of IGF‐1 in human peripheral blood lymphocytes

et al. 2014

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Insulin-like growth factor-1 (IGF-1) mediates some of growth hormone anabolic functions through its receptor, IGF-1R. Following ligand binding, intracellular signaling pathways are activated favouring proliferation, cell survival, tissue growth, development, and differentiation. IGF-1 is included in the World Anti-Doping Agency Prohibited List. While the evidence for IGF-1 as performance-enhancing substrate in healthy humans is still weak, clinical studies demonstrated that the endogenous growth hormone/IGF-1 excess is associated with cardiovascular implications. Previously, we demonstrated that human peripheral blood lymphocytes represent a suitable system to identify a gene signature, related to dihydrotestosterone or IGF-1 abuse, independent from the type of sport. In addition, in a proteomic study, we demonstrated that dihydrotestosterone hyperdosage affects cell motility and apoptosis. Here, we investigate the doping action of IGF-1 by means of a differential proteomic approach and specific protein arrays, revealing an active cytoskeletal reorganization mediated by Stat-1; moreover, IGF-1 stimulation produces a sustained activation of different signaling pathways as well as an overproduction of cytokines positively related to immune response and inflammation. In conclusion, these data indicate that, following IGF-1 hyperdosage, circulating peripheral blood lymphocytes could be more prone to transendothelial migration.

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Section: Proteins Identified As Single Speciesmentioning

confidence: 99%

Insulin‐like growth factor 1 receptor signaling induced by supraphysiological doses of IGF‐1 in human peripheral blood lymphocytes

et al. 2014

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“…In their earlier phase, oxidative stress and infl ammation contribute to insulin resistance and hyperinsulinemia, whereas chronic exposure appears to cause mitochondrial and pancreatic dysfunction in the more advanced stages, subsequently resulting in metabolic abnormalities, such as dyslipidemia and hyperglycemia (3,4) . Furthermore, hyperinsulinemia stimulates cellular iron uptake through transferrin receptor externalization and gene expression of ferritin (9,10) . Thus, the initiation of insulin resistance and hyperinsulinemia by iron excess enhances intracellular translocation of iron and aggravates iron toxicity.…”

mentioning

confidence: 99%

Serum ferritin concentrations predict incidence of metabolic syndrome in rural Korean adults

Yoon¹,

Linton

Koh

et al. 2012

Clinical Chemistry and Laboratory Medicine (CCLM)

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Keywords: ferritin; infl ammation; insulin resistance; metabolic syndrome; oxidative stress.Serum ferritin concentrations refl ect iron storage in the body and are associated with systemic infl ammation and insulin function. Body iron, a transitional ion, can play a role in the conversion of less-reactive free radicals to more-reactive hydroxyl radicals, which acts as oxidative stressors at multiple cellular targets (1) . Concentrations of serum ferritin, an acute phase protein, are elevated in infl ammatory environments, which contribute to cardiometabolic diseases (2) . Elevated serum ferritin and iron concentrations are related to insulin resistance and hyperinsulinemia in the early phases, while chronic exposure to these elevated concentrations subsequently results in insuffi cient insulin production in the more advanced phases (3,4) . Due to the lack of prospective evidence on the independent relationship between serum ferritin concentrations and the incidence of metabolic syndrome (MetS), we conducted this study to determine whether elevated serum ferritin concentrations could predict MetS development.The Korean Genomic Rural Cohort (KGRC) is an ongoing multicenter cohort study with 10,114 adult participants aged 40 years and older. Participants who have been diagnosed with any malignancy or have incomplete laboratory data were excluded in this study. Participants who were taking antihypertensive, anti-diabetic, or anti-dyslipidemic medication, or dietary supplements including iron, were also excluded. Finally, 865 eligible subjects without MetS at baseline were selected. All participants gave written informed consent and this study was approved by the Institutional Review Board of Wonju Christian Hospital.Trained medical staff performed physical examinations following standard procedures. A blood sample was obtained after overnight fasting. The samples were stored at -80 ° C and were analyzed within 1 week. More detailed methods of quality and accuracy control were described in our previous article (5) .We defi ned MetS using a harmonizing defi nition published in 2009, defi ned as the presence of three or more of the following criteria (6) : waist circumference, ≥ 90 cm in men and ≥ 80 cm in women; systolic blood pressure ≥ 130 mm Hg, diastolic blood pressure ≥ 85 mm Hg, or on anti-hypertensive medication for hypertension; fasting plasma glucose concentrations ≥ 5.5 mmol/L or on anti-diabetic medication for hyperglycemia; triglyceride concentrations ≥ 1.7 mmol/L or on triglyceridelowering medication for hypertriglyceridemia; high-density lipoprotein cholesterol (HDL-C) concentrations, < 1.0 mmol/L in men and < 1.3 mmol/L in women, or on drug treatment for reduced HDL-C concentrations. A cut-off point for waist circumference has been adopted specifi cally for Asian populations. Insulin resistance was calculated using the homeostasis model assessment of insulin resistance (HOMA-IR).Serum ferritin concentrations were categorized into tertile groups for men, and pre-or post-menopausal women. Analysis of varia...

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“…Therefore, the results prove that the prokaryotic RNA obtained by ACP-based RT-PCR contained polyadenylic acid without adding poly(A). ACP-based RT-PCR technology is an easy differential genes analysis technology that uses agarose gel electrophoresis, which is widely used to identify DEGs in response to different conditions [37][38][39]. In this study, ACP-based RT-PCR was used to detect DEGs in response to pBD2 treatments.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Mechanism of pBD2 against Staphylococcus aureus

Zhang

Gao

et al. 2020

Molecules

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Antimicrobial peptides (AMPs) show high antibacterial activity against pathogens, which makes them potential new therapeutics to prevent and cure diseases. Porcine beta defensin 2 (pBD2) is a newly discovered AMP and has shown antibacterial activity against different bacterial species including multi-resistant bacteria. In this study, the functional mechanism of pBD2 antibacterial activity against Staphylococcus aureus was investigated. After S. aureus cells were incubated with different concentrations of pBD2, the morphological changes in S. aureus and locations of pBD2 were detected by electron microscopy. The differentially expressed genes (DEGs) were also analyzed. The results showed that the bacterial membranes were broken, bulging, and perforated after treatment with pBD2; pBD2 was mainly located on the membranes, and some entered the cytoplasm. Furthermore, 31 DEGs were detected and confirmed by quantitative real-time PCR (qRT-PCR). The known functional DEGs were associated with transmembrane transport, transport of inheritable information, and other metabolic processes. Our data suggest that pBD2 might have multiple modes of action, and the main mechanism by which pBD2 kills S. aureus is the destruction of the membrane and interaction with DNA. The results imply that pBD2 is an effective bactericide for S. aureus, and deserves further study as a new therapeutic substance against S. aureus.

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Insulin stimulates gene expression of ferritin light chain in osteoblast cells

Cited by 11 publications

References 55 publications

Insulin‐like growth factor 1 receptor signaling induced by supraphysiological doses of IGF‐1 in human peripheral blood lymphocytes

Insulin‐like growth factor 1 receptor signaling induced by supraphysiological doses of IGF‐1 in human peripheral blood lymphocytes

Serum ferritin concentrations predict incidence of metabolic syndrome in rural Korean adults

Antimicrobial Mechanism of pBD2 against Staphylococcus aureus

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