Insulin signaling pathway assessment by enhancing antioxidant activity due to morin using in vitro rat skeletal muscle L6 myotubes cells

Issac, Praveen Kumar; Karan, Rupmanjari; Guru, Ajay; Pachaiappan, Raman; Arasu, Mariadhas Valan; Al-Dhabi, Naïf Abdullah; Choi, Ki Choon; Harikrishnan, Ramasamy; Raj, Jesu Arockia

doi:10.1007/s11033-021-06580-x

Cited by 36 publications

(19 citation statements)

References 70 publications

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“…90 Among the antioxidant enzymes, SOD serves as the first line of defense in the antioxidant system, converting superoxide radicals to H 2 O 2 , while CAT catalyzes the conversion of H 2 O 2 to water. 44,91,92 The enzyme catalase plays an important role in preventing lipid peroxidation by protecting cells against oxidative stress. 93 Since the brain being a lipid rich organ with high energy consumption, it more prone to oxidative mediated cell injury.…”

Section: Discussionmentioning

confidence: 99%

“…All of these antioxidant enzymes are known to protect neurons against oxidative stress mediated injury 90 . Among the antioxidant enzymes, SOD serves as the first line of defense in the antioxidant system, converting superoxide radicals to H 2 O 2 , while CAT catalyzes the conversion of H 2 O 2 to water 44,91,92 . The enzyme catalase plays an important role in preventing lipid peroxidation by protecting cells against oxidative stress 93 .…”

Section: Discussionmentioning

confidence: 99%

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Embryonic exposure to butylparaben and propylparaben induced developmental toxicity and triggered anxiety‐like neurobehavioral response associated with oxidative stress and apoptosis in the head of zebrafish larvae

Lite

Guru

Juliet

et al. 2022

Environmental Toxicology

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Parabens are synthetic antimicrobial compounds used as a preservative for extending the shelf life of food, pharmaceutical and cosmetic products. The alkyl chain length of the paraben esters positively correlates with their antimicrobial property. Hence, long-chain paraben esters, namely butylparaben and propylparaben, are used in combination as they have better solubility and antimicrobial efficacy. Extensive use of parabens has now resulted in the ubiquitous presence of these compounds in various human and environmental matrices. During early life, exposure to environmental contaminants is known to cause oxidative-stress mediated apoptosis in developing organs. The brain being one of the high oxygen-consuming, metabolically active and lipid-rich organ, it is primarily susceptible to reactive oxygen species (ROS) and lipid peroxidation (LP) induced neuronal cell death. The primary cause for the impairment in cognitive and emotional neurobehvioural outcomes in neurodegenerative disease was found to be associated with neuronal apoptosis. The present study aimed to study butylparaben and propylparaben's effect on zebrafish during early embryonic stages. Besides this, the association between alteration in anxiety-like neurobehavioral response with oxidative stress and antioxidant status in head region was also studied. The study results showed variation in the toxic signature left by butylparaben and propylparaben on developmental parameters such as hatching rate, survival and non-lethal malformations in a time-dependent manner. Data from the light-dark preference test showed embryonic exposure to butylparaben and propylparaben to trigger anxiety-like behavior in zebrafish larvae. In addition, a significant increase in intracellular ROS and LP levels correlated with suppressed antioxidant enzymes: superoxide dismutases (SOD), catalases (CAT), Glutathione peroxidase (GPx), glutathione S-transferase (GST), and Glutathione (GSH) activity in the head region of the zebrafish larvae. Acetylcholinesterase (AChE) activity was also suppressed in the exposed groups, along with increased nitric oxide production. The overall observations show increased oxidative stress indices correlating with upregulated expression of apoptotic cells in a dose-dependent manner. Collectively, our findings reveal butylparaben and propylparaben as an anxiogenic neuroactive Christy Lite, Ajay Guru, and Melita Juliet contributed equally to this study.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Embryonic exposure to butylparaben and propylparaben induced developmental toxicity and triggered anxiety‐like neurobehavioral response associated with oxidative stress and apoptosis in the head of zebrafish larvae

Lite

Guru

Juliet

et al. 2022

Environmental Toxicology

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“…Prebiotics are naturally produced and environmentally friendly, and their administration has been cited as a potential method to prevent fish infections in the aquaculture industry [ 8 , 9 , 10 ]. Prebiotics are defined as non-digestible food ingredients that beneficially affect the host’s health by selectively stimulating the growth and/or activity of beneficial microflora in the colon.…”

Section: Introductionmentioning

confidence: 99%

Effects of Jerusalem Artichoke (Helianthus tuberosus) as a Prebiotic Supplement in the Diet of Red Tilapia (Oreochromis spp.)

Trullàs

Sewaka

Rodkhum

et al. 2022

Animals

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The aim of the present study was to evaluate the effects of a Jerusalem artichoke-supplemented diet on the blood chemistry, growth performance, intestinal morphology, expression of antioxidant-related genes, and disease resistance against Aeromonas veronii challenge in juvenile red tilapia. A completely randomized design (CRD) was followed to feed red tilapias with three experimental diets: control, 5.0 g/kg JA-supplemented (JA5), or 10.0 g/kg JA-supplemented (JA10) diets in triplicates for 4 weeks. The results revealed that the growth performance, weight gain (WG), specific growth rate (SGR), and average daily gain (ADG) of fish fed diets JA5 and JA10 were significantly higher (p < 0.05) than those of fish fed the control diet. Fish fed the control diet had significantly higher T-bilirubin, D-bilirubin, and ALT in blood serum than fish fed JA5 and JA10, as well as higher BUN than fish fed JA5. The number of goblet cells in the proximal and distal parts of the intestine revealed that the number of acid, neutral, and double-staining mucous cells of fish fed diets JA5 and JA10 was significantly higher (p < 0.05) than in fish fed the control diet. The diets including the prebiotic (JA5 and JA10) were associated with a significant increase in the expression of gpx1 and gst antioxidant-related genes and disease resistance against A. veronii in juvenile red tilapia. Therefore, JA5 and JA10 can be employed as promising prebiotics for sustainable red tilapia farming.

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“…GLUT4 is downregulated in adipocytes condition, and this is one of the first steps in the pathogenesis of insulin resistance. [58][59][60] Based on this, the glucose uptake activity of WL15 in zebrafish larvae was investigated. In comparison to the control group, the BPA larvae group showed a decrease in glucose uptake (Figure 9).…”

Section: Discussionmentioning

confidence: 99%

Exposure to environmental pollutant bisphenol A causes oxidative damage and lipid accumulation in Zebrafish larvae: Protective role of WL15 peptide derived from cysteine and glycine‐rich protein 2

Guru

Arockiaraj

2022

J Biochem & Molecular Tox

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Humans are exposed to obesity causing Bisphenol A in various ways, especially through diet and food containers. Bioactive peptides are already reported to have antioxidant, antidiabetic, and antiobesity properties, which can mimic the role of mediators involved in obesity prevention. The protective effect of a short molecule or peptide, WL15 from cysteine and glycine‐rich protein 2 of a teleost of aquatic resource on Bisphenol A (BPA)‐induced lipid accumulation in zebrafish larvae was investigated. BPA exposure disrupted the antioxidant enzymes, apoptosis, and nitric oxide and led to changes in biochemical markers including alkaline phosphatase, lactate dehydrogenase, lipid peroxidation, glutathione S‐transferases, glutathione peroxidase, and reduced glutathione. However, WL15 inhibited the overproduction of oxidative stress, which correlates with its lipid‐lowering potential. BPA‐induced lipid accumulation in zebrafish showed an increase in triglyceride, cholesterol, and glucose level; simultaneously, WL15 treatment significantly reduced such accumulation in zebrafish. Evidenced by Oil red O staining and Nile red assay, WL15 inhibited lipid accumulation. At the same time, WL15 at 50 µM increases 2‐(N‐[7‐nitrobenz‐2‐oxa‐1,3‐diazol‐4‐yl]amino)‐2‐deoxy‐d‐glucose (2NBDG) glucose uptake in zebrafish. In addition, gene expression studies in zebrafish larvae demonstrated that the WL15 peptide could play a crucial role in preventing lipid accumulation by downregulating the expression of lipogenesis‐specific genes. These results revealed an interesting and novel property of WL15, suggesting its potential application in preventing lipid accumulation through the hypolipidemic and antioxidant properties.

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Insulin signaling pathway assessment by enhancing antioxidant activity due to morin using in vitro rat skeletal muscle L6 myotubes cells

Cited by 36 publications

References 70 publications

Embryonic exposure to butylparaben and propylparaben induced developmental toxicity and triggered anxiety‐like neurobehavioral response associated with oxidative stress and apoptosis in the head of zebrafish larvae

Embryonic exposure to butylparaben and propylparaben induced developmental toxicity and triggered anxiety‐like neurobehavioral response associated with oxidative stress and apoptosis in the head of zebrafish larvae

Effects of Jerusalem Artichoke (Helianthus tuberosus) as a Prebiotic Supplement in the Diet of Red Tilapia (Oreochromis spp.)

Exposure to environmental pollutant bisphenol A causes oxidative damage and lipid accumulation in Zebrafish larvae: Protective role of WL15 peptide derived from cysteine and glycine‐rich protein 2

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