Insulin-sensitizing and insulin-mimetic activities of Sarcopoterium spinosum extract

Rozenberg, Konstantin; Smirin, Polina; Sampson, Sanford R.; Rosenzweig, Tovit

doi:10.1016/j.jep.2014.05.030

Cited by 14 publications

(29 citation statements)

References 56 publications

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“…In our previous study, we demonstrated that Akt is not phosphorylated on ser473, a phosphorylation site recognized as required for the activation of this kinase, following treatment with root extract of S. spinosum . On the other hand, Akt was found to be activated by S. spinosum , as found by the membranal/nuclear translocation of this protein, and the phosphorylation of its downstream proteins [12]. These results indicate the presence of a unique mode of Akt activation by S. spinosum root extract.…”

Section: Discussionmentioning

confidence: 99%

“…3 T3-L1 pre-adipocytes were cultured and induced to differentiate as described before [12]. 3 T3-L1 adipocytes were used for experiments 14 days after the initiation of differentiation, when 80–90% of cells exhibited adipocyte morphology.…”

Section: Methodsmentioning

confidence: 99%

“…Experiments were performed on differentiated myotubes. L6 differentiation was induced as described in our previous studies [12]. …”

Section: Methodsmentioning

confidence: 99%

“…In addition, insulin secretion was enhanced in-vitro, suggesting a stimulatory effect on pancreatic β-cells [11]. In-vivo studies revealed that the glucose lowering properties of S. spinosum are accompanied by lower serum insulin levels and improved insulin tolerance [11, 12], indicating that when S. spinosum is administrated to the animal, the improvement in insulin sensitivity enables a reduction, rather than a stimulation, of insulin release.…”

Section: Introductionmentioning

confidence: 99%

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Anti-diabetic activity of aerial parts of Sarcopoterium spinosum

Elyasiyan

Nudel

Skalka

et al. 2017

BMC Complement Altern Med

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Background Sarcopoterium spinosum (S. spinosum) is used by Bedouin medicinal practitioners for the treatment of diabetes. While the anti-diabetic activity of S. spinosum root extract was validated in previous studies, the activity of aerial parts of the same plants has not been elucidated yet. The aim of this study was to clarify the glucose lowering properties of the aerial parts of the shrub.MethodsAnti-diabetic properties were evaluated by measuring the activity of carbohydrate digesting enzymes, glucose uptake into 3 T3-L1 adipocytes, and insulin secretion. Insulin signaling cascade was followed in L6 myotubes using Western blot and PathScan analysis.ResultsActivity of α-amylase and α-glucosidase was inhibited by extracts of all S. spinosum organs. Basal and glucose-induced insulin secretion was measured in Min6 cells and found to be enhanced as well. Glucose uptake was induced by all S. spinosum extracts, with roots found to be the most effective and fruits the least.The effect of S. spinosum on Akt phosphorylation was minor compared to insulin effect. However, GSK3β and PRAS40, which are downstream elements of the insulin cascade, were found to be highly phosphorylated by S. spinosum extracts. Inhibition of PI3K and Akt, but not AMPK and ERK, abrogated the induction of glucose uptake by the aerial parts of the shrub.ConclusionThe aerial organs of S. spinosum have anti-diabetic properties and may be used as a basis for the development of dietary supplements or to identify new agents for the treatment of type 2 diabetes.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Experiments were performed on differentiated myotubes. L6 differentiation was induced as described in our previous studies [12]. …”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Anti-diabetic activity of aerial parts of Sarcopoterium spinosum

Elyasiyan

Nudel

Skalka

et al. 2017

BMC Complement Altern Med

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“…Sarcopoterium spinosum (S. spinosum) is a chamaephyte of the Rosaceae family, which has been used in Bedouin folk medicine for its antidiabetic effect [15]. In our previous studies, we validated its glucose lowering properties, demonstrating an activation of the insulin signaling cascade, leading to an induction of glucose uptake in myotubes, hepatocytes and adipocytes [16,17]. These data were also supported by a set of in vivo experiments, demonstrating an improved glucose tolerance and insulin sensitivity in high-fat diet-fed mice and in genetic-prone type 2 diabetic mice (KK-Ay mice).…”

Section: Introductionmentioning

confidence: 92%

Sarcopoterium spinosum Inhibited the Development of Non-Alcoholic Steatosis and Steatohepatitis in Mice

2019

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Non-alcoholic fatty liver disease (NAFLD) is a comorbidity of obesity, which gradually develops from hepatic steatosis into steatohepatitis (NASH) and eventually even into fibrosis or hepatic carcinoma. To date, there has been no specific and effective treatment for NAFLD. Sarcopoterium spinosum extract (SSE) was found to improve insulin sensitivity. Recognizing the intimate link between insulin resistance and NAFLD, the aim of this study was to investigate the effectivity of SSE in the prevention and management of NAFLD at various severities. SSE was given to high-fat diet (HFD)-fed mice (steatosis model) or to mice given a Western diet (WD) in the short or long term (NASH prevention or treatment, respectively). SSE reduced body weight accumulation, improved glucose tolerance and insulin sensitivity and prevented the development of hepatic steatosis. SSE also blocked the progression of liver disease toward NASH in a dose-dependent manner. The expression of genes involved in lipid metabolism, inflammation, and antioxidant machinery was regulated by SSE in both models of steatosis and NASH development. However, SSE failed to reverse the hepatic damage in the advanced model of NASH. In summary, SSE might be considered as a botanical supplement for the prevention and treatment of hepatic steatosis, and for slowing the deterioration toward NASH.

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