Insulin-sensitive glucose transporter transcript levels in calf muscles assessed with a bovine GLUT4 cDNA fragment

Hocquette, Jean-François; Graulet, Benoı̂t; Castiglia-Delavaud, C.; Bornes, F.; Lepetit, Nathalie; Ferré, Pascal

doi:10.1016/1357-2725(96)00013-1

Cited by 18 publications

(33 citation statements)

References 31 publications

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“…As in other species, GLUT4 mRNA was detected in the heart, muscles and adipose tissue but not in non insulin-responsive tissues from cattle using either human [100] or bovine cDNA probes [3,49]. Unlike human organs, the liver and kidney of lactating cows express a high GLUT5 mRNA level [100], the physiological role of this transporter still being a matter of conjoncture [70].…”

Section: Immunological Studiesmentioning

confidence: 77%

“…However, many authors have considerable difficulties with this technique, whatever the animal species due to several practical problems. Firstly, some non-specific bands may be detected either with a non-immune serum [96], or even with polyclonal antibodies specifically raised against GLUTs [41,49,85]. Secondly, a confident detection of the GLUT proteins is made difficult because of the inconsistency of their electrophoretic behavior due to the heterogeneity of the protein glycosylation, as for GLUT1 [32,61], GLUT3 [32] and GLUT4 [50,96].…”

Section: Immunological Studiesmentioning

confidence: 99%

“…The recent cloning of GLUT4 probes in pigs [22,58], sheep [15], and cattle [3,49] will allow scientists to further understand the regulation of glucose transport in insulinsensitive tissues of livestock animals. Only the bovine GLUT4 was completely sequenced.…”

Section: Immunological Studiesmentioning

confidence: 99%

“…Northern blot hybridization was used to demonstrate the presence of a major transcript for GLUT4 at 2.7-2.8 kb in cattle [3,49], pigs [30], rats and mice tissues. The larger size of the GLUT4 message in human tissues (3.5 kb) is thought to be due to differences in size of the 3' UTR (for review, see [72]).…”

Section: Immunological Studiesmentioning

confidence: 99%

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Facilitative glucose transporters in livestock species

Hocquette

Abé

2000

Reprod. Nutr. Dev.

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-The study of facilitative glucose transporters (GLUT) requires carefully done immunological experiments and sensitive molecular biology approaches to identify the various mechanisms which control GLUT expression at the RNA and protein levels. The cloning of species-specific GLUT cDNAs showed that GLUT4 and GLUT1 diverge less among species than other GLUT isoforms. The key role of GLUT in glucose homeostasis has been demonstrated in livestock species. In vitro studies have suggested specific roles of GLUT1 and GLUT3 in avian cells. In vivo studies have demonstrated a regulation of GLUTs (especially of GLUT4) by nutritional and hormonal factors in pigs and cattle, in lactating cows and goats and throughout the foetal life in the placenta and tissues of lambs and calves. All these results suggest that any changes in GLUT expression and activity (such as GLUT4 in muscles) could modify nutrient partitioning and tissue metabolism, and hence, the qualities of animal products (milk, meat).glucose transporter / pig / ruminant / poultry Résumé -Les transporteurs du glucose à diffusion facilitée chez les animaux de ferme. L'étude des transporteurs du glucose à diffusion facilitée (GLUT) nécessite des techniques en immunologie ou en biologie moléculaire précises et sensibles pour étudier la régulation de leur expression au niveau ARNm ou protéique. Le clonage d'ADNc des différents GLUT a montré que GLUT4 et GLUT1 divergent moins entre espèces que les autres isoformes de GLUT. Le rôle important des GLUT dans le contrôle de l'homéostasie du glucose a été démontré chez les animaux de ferme. Des études in vitro ont suggéré un rôle spécifique de GLUT1 et de GLUT3 dans les cellules aviaires. Des études in vivo ont mis en évidence une régulation des GLUT (notamment GLUT4) par des facteurs nutritionnels ou hormonaux chez le porc et le bovin, chez la vache ou la chèvre en lactation, et tout au long de la vie foetale dans le placenta et les tissus de l'agneau ou du veau. L'ensemble de ces résultats suggère que tout changement dans l'expression et l'activité des GLUT (tel que de GLUT4 dans les muscles) peut modifier la répartition des nutriments et le métabolisme tissulaire, et affecter ainsi la qualité des produits animaux (lait, viande).transporteur du glucose / porc / ruminant / volaille Reprod. Nutr. Dev. 40 (2000) 517-533 517

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Section: Immunological Studiesmentioning

confidence: 77%

Section: Immunological Studiesmentioning

confidence: 99%

Section: Immunological Studiesmentioning

confidence: 99%

Section: Immunological Studiesmentioning

confidence: 99%

See 2 more Smart Citations

Facilitative glucose transporters in livestock species

Hocquette

Abé

2000

Reprod. Nutr. Dev.

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“…The bGHR DNA fragment and a mouse β-cDNA probe (1 kb) were labelled with α[ 32 P]-dCTP (3000 Ci·mmol -1 ) (ICN, France) to a specific activity of 10 8 cpm·µg -1 DNA using a random priming kit (Roche). Used as a control, a rat 18S ribosomal oligonucleotide probe was labelled at the 5'end with γ [ 32 P]-ATP using polynucleotide kinase as described by Hocquette et al [26]. Membranes of muscle mRNA were first hybridised with the 32 P GHR cDNA probe, then they were stripped off (boiling in 0.5% SDS), before re-hybridisation with the 32 P labelled 18S ribosomal oligonucleotide, then with the 32 P β-actin cDNA probe.…”

Section: Northern-blot Analysismentioning

confidence: 99%

Growth hormone receptor gene expression in the skeletal muscle of normal and double-muscled bovines during foetal development

Listrat

Hocquette²,

Picard³

et al. 2005

Reprod. Nutr. Dev.

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-The expression of the growth hormone receptor (GHR) gene was investigated in semitendinosus muscle during bovine foetal development in both normal and double-muscled Charolais foetuses which differ with respect to muscle development. Northern-blot analysis of foetal muscle RNA preparations with a GHR cDNA probe identified the 4.5 kb GHR mRNA as early as 130 days post-conception. In double-muscled animals, the expression of GHR mRNA increased from 130 to 210 days of gestation while it stayed stable in normal ones. It was significantly higher (P < 0.05) in double-muscled foetuses compared to normal ones from the second third of gestation. Northern-blot analysis of foetal muscle RNA preparations from both genotypes with a β-actin cDNA probe, revealed lower β-actin gene expression in double-muscled foetuses than in normal ones, suggesting a delay in the differentiation of muscle cells. In situ hybridisation revealed the localisation of specific GHR mRNA in muscle cells at all gestation stages analysed (130, 170, 210 days postconception) but not in connective tissue surrounding the muscle cells. At the adult stage, the hybridisation signal was also very high and observed in muscle cells only. These results show the ontogeny of GHR mRNA in bovine muscle and demonstrate a difference between normal and doublemuscled animals. muscle / bovine / receptor / growth hormone / in situ hybridisation

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Justification for antioxidant preconditioning (or how to protect insulin-mediated actions under oxidative stress)

Orzechowski

2003

J. Biosci.

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Insulin resistance is characterized by impaired glucose utilization in the peripheral tissues, accelerated muscle protein degradation, impaired antioxidant defences and extensive cell death. Apparently, both insulin and IGF-1 at physiological concentrations support cell survival by phosphatidylinositol 3 kinase-dependent and independent mechanisms. Postprandial hyperglycemia and hyperinsulinemia are found in insulin resistance, which accompanies the so-called noninsulin dependent diabetes mellitus (diabetes type 2). Evidence also indicates that increased susceptibility of muscle cells and cardiomycoytes to oxidative stress is among the harmful complications of insulin resistance and diabetes. Limited knowledge showing benefits of preconditioning with anti- oxidants (vitamin C, E, a-lipoic acid, N-acetylcysteine) in order to protect insulin action under oxidative stress prompted the author to discuss the theoretical background to this approach. It should be stressed that antioxidant preconditioning is relevant to prevention of both diabetes- and insulin resistance-associated side-effects such as low viability and cell deletion. Furthermore, antioxidant conditioning promises to provide higher efficacy for clinical applications in myoblast transfer therapy and cardiomyoplasty.

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Insulin-sensitive glucose transporter transcript levels in calf muscles assessed with a bovine GLUT4 cDNA fragment

Cited by 18 publications

References 31 publications

Facilitative glucose transporters in livestock species

Facilitative glucose transporters in livestock species

Growth hormone receptor gene expression in the skeletal muscle of normal and double-muscled bovines during foetal development

Justification for antioxidant preconditioning (or how to protect insulin-mediated actions under oxidative stress)

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