Insulin secretion dynamics of free and alginate-encapsulated insulinoma cells

Cheng, Shing-Yi; Constantinidis, Ioannis; Sambanis, Athanassios

doi:10.1007/s10616-006-9025-4

Cited by 8 publications

(4 citation statements)

References 32 publications

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“…The data show that APA‐encapsulated βTC‐tet cells cultured in the absence of TC displayed a glucose consumption increase due to continuous cellular proliferation, while APA beads cultured in the presence of TC maintained their initial metabolic activity for the experiment duration because cell proliferation was suppressed. These dynamics of regulated and unregulated growth of βTC‐tet cells are consistent with previously published data (34, 35). These data also demonstrate that labeling these model tissue‐engineered constructs in either the extracellular alginate matrix or the cells did not affect the gross metabolic activity of encapsulated cells.…”

Section: Resultssupporting

confidence: 92%

Use of magnetic nanoparticles to monitor alginate‐encapsulated βTC‐tet cells

Constantinidis

Grant

Simpson

et al. 2009

Magnetic Resonance in Med

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Section: Resultssupporting

confidence: 92%

Use of magnetic nanoparticles to monitor alginate‐encapsulated βTC‐tet cells

Constantinidis

Grant

Simpson

et al. 2009

Magnetic Resonance in Med

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“…The beads used in these insulin secretion experiments were not returned to the main culture. The 30 min secretion period was sufficient to capture the stimulated secretory response (Cheng et al 2006). Secretion rates were calculated in pmol insulin per hour and per 10 8 cells.…”

Section: 0 Materials and Methodsmentioning

confidence: 99%

Limited beneficial effects of perfluorocarbon emulsions on encapsulated cells in culture: Experimental and modeling studies

Goh

Gross

Simpson

et al. 2010

Journal of Biotechnology

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Due to the high solubility of oxygen in perfluorocarbons (PFCs), these compounds have been explored for improved cell and tissue oxygenation. The goal of this study is to investigate the effects of a PFC emulsion on cellular growth and function in a tissue engineered construct. A perfluorotributylamine (PFTBA) emulsion was co-encapsulated at 10 vol% with mouse βTC-tet insulinoma cells in calcium alginate beads and cultured under normoxic and severely hypoxic conditions. The number of metabolically active cells and the induced insulin secretion rate were measured over time for up to 16 days. Results showed no significant effect of PFTBA relative to the PFTBA-free control. The alginate-PFC-cell system was also modeled mathematically, and simulations tracked the number of viable cells over time under the same conditions used experimentally. Simulations revealed only a small, likely experimentally undetectable difference in cell density between the PFC-containing and PFC-free control beads. It is concluded that PFTBA up to 10 vol% has no significant effect on the growth and function of encapsulated βTC-tet cells under normoxic and hypoxic conditions. KeywordsPerfluorotributylamine; βTC-tet; encapsulated cells; normoxia; hypoxia Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. NIH Public Access NIH-PA Author ManuscriptNIH-PA Author Manuscript NIH-PA Author Manuscript IntroductionA major challenge in developing and implementing tissue engineered substitutes is ensuring sufficient oxygenation of cells within constructs, as oxygen is a significant parameter affecting cell viability and function (Gross et al. 2007b;Papas et al. 2000;Papas et al. 1999). This is especially true for substitutes which rely on diffusion for the transport of dissolved oxygen (DO). Therefore, a means of enhancing oxygen delivery by increasing the solubility and/or diffusivity of DO within a construct might prove beneficial and desirable.The high solubility of oxygen in perfluorocarbons (PFCs) has led investigators to explore their use in the form of a non-aqueous phase or an emulsion, as a blood substitute, in organ preservation, and in tissue engineered devices. It is reasonable to expect a positive effect in systems where the PFC phase is convectively transported between an oxygenator and a culture or tissue where oxygen is delivered and used. This has been confirmed experimentally, as in the study of Radisic et al. who demonstrated that adding the PFC emulsion, Oxygent, to medium perfusing a parallel plate cardiac bioreactor increased the DO concentration throughout the bioreactor and conseque...

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“…Encapsulation has been intensively tested as a perspective tool for immunoisolation of transplanted pancreatic islets [55]. It was shown that encapsulation in alginate beads does not affect the insulin secretion profile in vitro [55,56].…”

Section: +mentioning

confidence: 99%

Mechanism of Ethanol-Induced Insulin Secretion from INS-1 and INS-1E Tumor Cell Lines

Hafko

Orečná

Bačová

et al. 2009

Cell Physiol Biochem

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Alcohol causes reactive hypoglycemia by attenuating the release of counter regulatory hormones, redistribution of pancreatic blood flow and direct stimulation of insulin secretion. Objective of this study was characterization of ethanol-induced insulin secretion. Signaling of ethanol- and glucose-induced insulin release from INS-1 and INS-1E cells was compared. Both cell lines responded similarly to all experimental interventions. In contrast to glucose, ethanol-induced insulin secretion was not hindered in calcium depleted medium or by addition of 10 μM BAPTA/AM (intracellular chelator). Inhibitor of protein kinase C Bisindolylmaleimide (3 μM) abolished glucose- but not ethanol-induced insulin secretion. Tetanus toxin (20 nM), inhibitor of SNARE proteins complex formation, blocked ethanol-induced insulin secretion. Both 5 mM N-ethylamaleimide and 10 μM ZnCl₂ (inhibitor of protein tyrosine phosphatases), which block disassembly of SNARE complexes and their further participation in exocytosis, increased basal insulin secretion. In contrast to glucose, already high insulin secretion was further increased after ethanol stimulation in either treatment. Conclusion: Signaling of ethanol-induced insulin secretion from INS-1 and INS-1E cell lines bypasses calcium and PKC involving steps, is sensitive to tetanus toxin but resistant to N-ethymaleimide and ZnCl₂. An extra pool of secretory vesicles not available for glucose is exploited for exocytosis after ethanol stimulation.

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Insulin secretion dynamics of free and alginate-encapsulated insulinoma cells

Cited by 8 publications

References 32 publications

Use of magnetic nanoparticles to monitor alginate‐encapsulated βTC‐tet cells

Use of magnetic nanoparticles to monitor alginate‐encapsulated βTC‐tet cells

Limited beneficial effects of perfluorocarbon emulsions on encapsulated cells in culture: Experimental and modeling studies

Mechanism of Ethanol-Induced Insulin Secretion from INS-1 and INS-1E Tumor Cell Lines

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