Abstract:OBJECTIVE: To test the efficiency of direct spherification in supporting differentiation of mouse embryonic stem cells (mESCs) into meiotic male germ cells.DESIGN: Biological 3D scaffolding was utilized to culture and differentiate mESCs. Two main methods were investigated: 1) mESCs were injected into pre-formed spheres (IN), and 2) a suspension of mESCs was directly encapsulated (DE) into spheres. To coax differentiation, spheres were bathed in a growth factor cocktail. After mechanical breaching of the spher… Show more
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