Insulin regulation of lipoprotein lipase (LPL) activity and expression in gilthead sea bream (Sparus aurata)

Albalat, Amaya; Saera-Vila, Alfonso; Capilla, Encarnación; Gutiérrez, Joaquím; Pérez‐Sánchez, Jaume; Navarro, Isabel

doi:10.1016/j.cbpb.2007.05.004

Cited by 96 publications

(70 citation statements)

References 51 publications

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“…Changes in LPL activity have not always been accompanied by parallel changes in mRNA levels (Lee et al, 1998;Bergo et al, 1996). In gilthead sea bream, LPL activity changes were followed by an increase in LPL mRNA levels in the adipose tissue but the changes in LPL expression showed a time lag (Albalat et al, 2007).…”

Section: Tablementioning

confidence: 99%

“…In gilthead sea bream, the effects of season and dietary composition on LPL gene expression in different tissues were studied (Saera-Vila et al, 2005). Albalat et al (2006Albalat et al ( , 2007 examined the nutritional and insulin regulation of LPL activity in gilthead sea bream and rainbow trout. These studies indicated that LPL was regulated in response to the physiological state of the fish.…”

Section: Introductionmentioning

confidence: 99%

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Effects of dietary lipid levels on growth, survival and lipid metabolism during early ontogeny of Pelteobagrus vachelli larvae

et al. 2010

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Section: Tablementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of dietary lipid levels on growth, survival and lipid metabolism during early ontogeny of Pelteobagrus vachelli larvae

et al. 2010

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“…Adipogenesis is a well-regulated process controlled by the highly coordinated activation of various enzymes and transcription factors (Saez et al, 2009;Fu et al, 2014). As with lipogenic genes, the enzyme fatty acid synthase (FAS) is key to the process of fatty acid synthesis, catalyzing the synthesis of long-chain fatty acids through the condensation of acetyl-coenzyme A (CoA) and malonyl-CoA in a complex seven-step reaction (Albalat et al, 2007). Lipoprotein lipase (LPL) is an endothelial enzyme that catalyzes the hydrolysis of circulating triglyceride (TG) (Gondret et al, 2001;Andre et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Follicle-stimulating hormone increases the intramuscular fat content and expression of lipid biosynthesis genes in chicken breast muscle

Cui

Liu

et al. 2016

J. Zhejiang Univ. Sci. B

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Intramuscular fat (IMF) is a crucial factor in the quality of chicken meat. The genetic basis underlying it is complex. Follicle-stimulating hormone (FSH), well-known as an effector in reproductive tissues, was recently discovered to stimulate abdominal fat accumulation in chicken. The effect of FSH on IMF accumulation and the underlying molecular regulatory mechanisms controlling both IMF and abdominal fat deposition in vivo are largely unknown. In this study, two groups of chickens were treated with chicken FSH or a placebo. The lipid content of breast muscle, abdominal fat volume, and serum concentrations of FSH were examined. Related genes implicated in breast muscle and abdominal fat accumulation were also investigated. Compared to the control group, the triglyceride (TG) content of breast muscle and the percentage of abdominal fat in FSH-treated chickens were significantly increased by 64.9% and 56.5% (P<0.01), respectively. The FSH content in the serum of FSH-treated chickens was 2.1 times than that of control chickens (P<0.01). Results from quantitative real-time polymerase chain reaction (qRT-PCR) assays showed that relative expression levels of fatty acid synthase (FAS), lipoprotein lipase (LPL), diacylglycerol acyltransferase 2 (DGAT2), adipocyte fatty acid binding protein (A-FABP), and peroxisome proliferator-activated receptor γ (PPARγ) were significantly upregulated in breast muscle following FSH treatment (P<0.01). Treatment with FSH also significantly increased relative expression levels of FAS, LPL, DGAT2, A-FABP, and PPARγ in abdominal fat tissue (P<0.05). The results of principal component analysis (PCA) for gene expression (breast muscle and abdominal fat) showed that the control and FSH treatment groups were well separated, which indicated the reliability of the data. This study demonstrates that FSH plays an important role in IMF accumulation in female chickens, which likely involves the regulation of biosynthesis genes related to lipid metabolism.

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“…By contrast, insulin (INS) is lipogenic in mammals and fish as well as directly antilipolytic (via dephosphorylation of HSL) (Harmon et al 1993, Albalat et al 2007, Chaves et al 2011. The effects of GH define on lipid metabolism are complex, and short-term INS-like (antilipolytic) and long-term anti-INS-like (lipolytic) effects have been reported in mammals (Carrel & Allen 2000, Chaves et al 2011.…”

Section: Introductionmentioning

confidence: 99%

PKC and ERK mediate GH-stimulated lipolysis

Bergan

Kittilson

Sheridan

2013

Journal of Molecular Endocrinology

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GH regulates several physiological processes in vertebrates, including the promotion of growth, an anabolic process, and the mobilization of stored lipids, a catabolic process. In this study, we used hepatocytes isolated from rainbow trout (Oncorhynchus mykiss) as a model to examine the mechanism of GH action on lipolysis. GH stimulated lipolysis as measured by increased glycerol release in both a time-and a concentration-related manner. The promotion of lipolysis was accompanied by GH-stimulated phosphorylation of the lipolytic enzyme hormone-sensitive lipase (HSL). GH-stimulated lipolysis was also manifested by an increased expression of the two HSL-encoding mRNAs, HSL1 and HSL2. The signaling pathways that underlie GH-stimulated lipolysis were also studied. GH resulted in the activation of phospholipase C (PLC)/protein kinase C (PKC) and the MEK/ERK pathway, whereas JAK-STAT and the PI3K-Akt pathway were deactivated. The blockade of PLC/PKC and the MEK/ERK pathway inhibited GH-stimulated lipolysis and GH-stimulated phosphorylation of HSL as well as GH-stimulated HSL mRNA expression, whereas the blockade of JAK-STAT or the PI3K-Akt pathway had no effect on the activation of lipolysis or the expression of HSL stimulated by GH. These results indicate that GH promotes lipolysis by activating HSL and by enhancing the de novo expression of HSL mRNAs via the activation of PKC and ERK. These findings also suggest molecular mechanisms for activating the lipid catabolic actions of GH while simultaneously deactivating anabolic processes such as antilipolysis and the growth-promoting actions of GH.

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Insulin regulation of lipoprotein lipase (LPL) activity and expression in gilthead sea bream (Sparus aurata)

Cited by 96 publications

References 51 publications

Effects of dietary lipid levels on growth, survival and lipid metabolism during early ontogeny of Pelteobagrus vachelli larvae

Effects of dietary lipid levels on growth, survival and lipid metabolism during early ontogeny of Pelteobagrus vachelli larvae

Follicle-stimulating hormone increases the intramuscular fat content and expression of lipid biosynthesis genes in chicken breast muscle

PKC and ERK mediate GH-stimulated lipolysis

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